| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,634 | A→C | 100% | T108P (ACC→CCC) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,634 | 0 | A | C | 99.2% | 174.1 / ‑6.7 | 60 | T108P (ACC→CCC) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (31/25); minor base T (1/1); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTT > NC_000913/3041500‑3041773 | gCGACGCCAAAGGGAAAATGTGGAGCAAGTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATcgcg < 1:585804/143‑1 (MQ=255) gCCAGAGGTAAGATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAga < 2:574556/143‑1 (MQ=255) cAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTGTTCTCTAAAGTGCCCATCGCGCCAgacg > 1:111689/1‑143 (MQ=255) aaaGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTGTAAAGTGCCCATCGCGCCAgacga > 1:809523/1‑143 (MQ=255) aaGGTAAAAGGTGGGGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCCTGTTTTTTACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAgacgac < 2:394204/143‑1 (MQ=255) aGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCCGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATGGCGCCAGccgacg > 2:168232/1‑143 (MQ=255) tgtgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGtgtg > 1:1064357/1‑143 (MQ=255) tgtgGAGCAATTGACGTCTGTTCCGCGCCGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGtgtg < 1:16186/143‑1 (MQ=255) tgcggAGCGATCTACGCGTGTTCCCCTACGGCGGTGGCTTTGCATTGATTGAACGGCGCAGCGTGCGTCAACCCCACCTGACTGCCCTGAAAAAATATTCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGtgtg < 1:501447/140‑1 (MQ=255) tgGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCgtgaggt > 2:266476/1‑139 (MQ=255) aGCGATTTACGTCTGGTCCGCGGCGGCGAGGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTgctgct < 1:911945/143‑1 (MQ=255) aGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTgctgct > 1:271624/1‑143 (MQ=255) cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTg < 1:359976/143‑1 (MQ=255) cAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTg < 1:610358/143‑1 (MQ=255) gtttgtgTCGGTTCCGCGAGGGCGATGGCTTTGCATGGATTGAGCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAAGATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGgt < 2:382638/137‑1 (MQ=255) gcGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGTTCTTGGTTCCGGGCGACTAGCGTGTGCTGCTTGGTGTTg < 2:927799/142‑1 (MQ=255) gccTGTGCCGCGACGGCGGTGGCTCTGCCATCATCCACCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGcc < 1:703866/141‑1 (MQ=255) tCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCGTGGTGTTGCCg > 2:205452/1‑143 (MQ=255) gTTCCTCGACGGCGGTGCCTGTTCATGTCTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACGGAAAAAATATGCGGGATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtt < 2:983439/143‑1 (MQ=255) gTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGtt > 1:1031413/1‑143 (MQ=255) gcgACGGCTATGGCTTTGCATGGATGGACCGGCGCAGCGTGCGTGAACCGCAGCTCACTGAACTGAAAAAATATGCTGTATTCTCTAAAGTTCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAg < 2:170182/143‑1 (MQ=255) aCGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATAGGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGGTGCCGGCATTCAGgcg > 2:437979/1‑143 (MQ=255) gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGgcgcgcgc > 2:103107/1‑143 (MQ=255) gATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGCTTTCAGgcggctgc > 1:804034/1‑138 (MQ=255) tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAACTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGCCGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt > 2:874726/1‑143 (MQ=255) tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt > 1:24805/1‑143 (MQ=255) tttGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCt > 1:24810/1‑143 (MQ=255) cATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCa > 1:1037251/1‑143 (MQ=255) aTGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTTTTGCAGGTTAACAAGCGCGCGCCGCGCTGGCaa > 1:402643/1‑143 (MQ=255) aTTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAAtct > 1:175830/1‑143 (MQ=255) ggaCGGCGGAGCGTGCGGGAACCGCAGCTGACGGAACTGAAAAAATGTGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCtt < 2:258184/141‑1 (MQ=255) aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAACGTGCCCATCGCGCCAGACGACGACCGTGTGCTGCTTGCTGTTACCGGTTCTCAGCCGCGCGCCGCGCTGGCaaaacccctt > 2:428456/1‑136 (MQ=255) aaCGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGCTTTCAGGCGCGCGCCGCGCTGGCAAATCCCttt > 2:624954/1‑143 (MQ=255) gcAGAGTGCGGGAACCGCAGACGACTGAACTGAAAAAATCTGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACCTGCGTTTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGaa < 1:159809/143‑1 (MQ=255) cAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTGGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAAc < 1:739498/143‑1 (MQ=255) aGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAACTCTCTTTAGCGAACt > 1:965369/1‑143 (MQ=255) cgtgcgtgAACCGGAGCTGACTGAACTGAACAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGc < 2:30112/143‑1 (MQ=255) aggAACCGCAGCTGACTGAATTGAATAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCg < 1:248572/141‑1 (MQ=255) tgAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGCGGCCTTAGAGCCAGACGAAGAGCGATTTTAACGTTTTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTCGCGAACTGCCTTCga > 1:262096/1‑143 (MQ=255) aCCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCgaaag > 1:194922/1‑143 (MQ=255) cGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACGGCCTTCgaaagaa > 2:3906/1‑143 (MQ=255) ctgactgaACTGAAAACATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACa < 1:358170/143‑1 (MQ=255) gacagaACGGAAAAAAGATGCGGTATTCGCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAgg < 1:859157/143‑1 (MQ=255) ctgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTa < 1:103107/143‑1 (MQ=255) tgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCCAACCTCTTGAGCGAACTGCCTTCGAAAGCAACAGAGGGAg > 2:26751/1‑143 (MQ=255) tgaactgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTCCCGTCGAAAGCAACAGAGGtcg > 2:663114/1‑141 (MQ=255) aactgaaAAAATATGCGGTATTAAATAGGGGTTGCAGCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCGTTAGCGAACTGCCGTCGAAAGAAAAACAGGTAGTc > 2:687203/1‑143 (MQ=255) tgaaAACATATGCGGTATTCTCCAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa < 2:537963/143‑1 (MQ=255) tgaaAAAATATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCaaa < 1:643250/143‑1 (MQ=255) aaaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAaagaa > 1:675364/1‑143 (MQ=255) aaaaTATGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCACGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCGTCTAAAGAAAAACAGGTAGTCAaagaag > 1:569455/1‑143 (MQ=255) aaaaCATGCGGTGGTTTATGAAGTGGCCGTCGCGTTCGACTACGAGCGTGTGCTGCTTGGTGTTGCCCGTTTTCAGGCGCGCGCCGCGCTGGCAAATCGATTTAGCGACCTGCCTTCGAAAGAAAAACAGGTAGTCAaagaag < 2:905782/143‑1 (MQ=255) atGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGcgcg > 2:697655/1‑143 (MQ=255) atGCGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCGTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAcgcgcg > 2:487906/1‑143 (MQ=255) cGGTATTCTCTAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAACAACAGGGAGTCACAGGAGGCGCGAcc > 1:841412/1‑143 (MQ=255) ggTATTCTCTAAAGTGCCCATCACGCCAGACGACGAGCATGTGATGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGACAAAAAAACAGGTAGTCAAAGAAAGGCCGACCa > 2:618263/1‑143 (MQ=255) ttCTCTAAAGTGCCCGTCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACttt < 1:1100719/143‑1 (MQ=255) ctAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCTTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTa < 1:651424/143‑1 (MQ=255) tAAAGTGCCCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGCACTGCCTTCGCAAGCAAAACAGGTAGTCCACGAAGGCGCGACCACTTGGCTAt > 2:116663/1‑143 (MQ=255) gTGCCCGTCGCGCCGCACGACGCGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGtt < 2:649984/143‑1 (MQ=255) | GCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTGTTCCGCGACGGCGATGGCTTTGCATGGATTGAACGGCGCAGCGTGCGTGAACCGCAGCTGACTGAACTGAAAAAATATGCGGTATTCTCTAAAGTGACCATCGCGCCAGACGACGAGCGTGTGCTGCTTGGTGTTGCCGGTTTTCAGGCGCGCGCCGCGCTGGCAAATCTCTTTAGCGAACTGCCTTCGAAAGAAAAACAGGTAGTCAAAGAAGGCGCGACCACTTTGCTATGGTT > NC_000913/3041500‑3041773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |