Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,131,525 C→T 8.2% S295N (AGT→AAT) ‡ yghQ ← putative transport protein YghQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,131,5250CT 8.2% 117.9 / 2.8 49S295N (AGT→AAT) ‡yghQputative transport protein YghQ
Reads supporting (aligned to +/- strand):  ref base C (24/21);  new base T (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01

AGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGG  >  NC_000913/3131384‑3131666
                                                                                                                                             |                                                                                                                                             
aGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGCTGCGCGGATCTAAACGCATCACCTCTCGGTAAAGAcc                                                                                                                                              >  2:961747/1‑142 (MQ=255)
 gCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGAATCACCTCTGGGTAAAACCtt                                                                                                                                             >  2:51519/1‑143 (MQ=255)
  cgaggtGAGGGGCTCGCCGGCGATCAACCCCGCGAGCGCCCCCAGAATACCGAGTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACttt                                                                                                                                            <  2:679290/138‑1 (MQ=255)
  cGAAACGAGGGGCTCGCCGGCGATCACCACCGCGGGGGCCACCAGGATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACTACCTCGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACttt                                                                                                                                            <  2:899198/143‑1 (MQ=255)
           cGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGATGGTGCGCGGATCTAAACGCATCACCCCTGGGTCACATCTTTTTCCCCGcg                                                                                                                                   >  1:127290/1‑142 (MQ=255)
           cGGCTTGCCGACGACCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCGAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAGTTTTTACCCAgag                                                                                                                                   <  1:955531/143‑3 (MQ=255)
                tGCCGACGATCAACACCGCGAGCGCCACCAGAATGCCGATTCCGTCCGCGTGTAACCCGGACTGCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAGTTTTTACCCAgaggtgat                                                                                                                              <  1:257224/143‑8 (MQ=255)
                 gCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTCCGCGGATCTAACCGCATCACCTCCGGGCAAAACCTTTTACCCAGCAAACCCg                                                                                                                             >  2:1012372/1‑143 (MQ=255)
                   cgacgaTCAACACCGCGAGTGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATGCCCCCCGGGTAAAAACTTTTACCCAGCAAACCCGcc                                                                                                                           >  2:737838/1‑142 (MQ=255)
                      cgaTCAACACCGCGAGCGCCACCAGAATATCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTGCCCCGCAAACCCGCTGGc                                                                                                                        >  1:41906/1‑143 (MQ=255)
                        aTCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGAAAACCCGACTTCACACCGAACAACCACGGTCTGGTGGTGCGCGGATCCAAACGCATCACCCCAGGGTAAACACATTTACACAGCACACGAGCGGGCGt                                                                                                                      >  2:907412/1‑143 (MQ=255)
                               ccGCGGGCGCCGCTAGAACACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCTCCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCcggcg                                                                                                               <  1:55523/143‑1 (MQ=255)
                                 gtgggggcCACCAGCATACCGCTTGCGCCTGCTAGTAAACGCGACTTCACACCTAACAATCACGGTCTGGTGGTGCGCGGATCTAACCGCATCACCTCTGGGTAAAATCTTTTACCCAGCAAACCCGCTGGCGTTCCggcggc                                                                                                             <  2:190743/137‑1 (MQ=255)
                                   gAGTGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGt                                                                                                           <  1:737838/143‑1 (MQ=255)
                                    aGCGCCACCAGAATACCGATTCCGCCCGCCAGTACGCCGGTCTTGAATCCGCACGACCTCTGCCTGCCGCTGTGCGGATCTAAACGCAGCACCCCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTc                                                                                                          <  1:520910/143‑1 (MQ=255)
                                         caccaGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAGACCCGCTGGCGCGCCGACGGAGTCgagaa                                                                                                     >  1:54513/1‑140 (MQ=255)
                                         caccaGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCgaaga                                                                                                     >  1:576/1‑143 (MQ=255)
                                               aaTACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCa                                                                                               <  1:913405/143‑1 (MQ=255)
                                                ctctcGATTGCGCGCGCTCGTACACCGGACTTCACTCCTAACAACCACGTTCTGGTGGTGCGCGGATCTAAACGCATCACCGCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCAt                                                                                              <  2:37921/139‑1 (MQ=255)
                                                    cGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGATGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGAGTCGAAGAATGTCATGGCg                                                                                          >  2:365223/1‑143 (MQ=255)
                                                      aTTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTACTGGCgct                                                                                        >  2:280636/1‑141 (MQ=255)
                                                      aTTCCGCCCGCCAGCAACCCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGAt                                                                                        <  1:60296/143‑1 (MQ=255)
                                                             ccgccgGTAAGCCGGACTTCACACCCAACATCCGCGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAAt                                                                                 <  2:821052/143‑1 (MQ=255)
                                                              cgccAGTAAACCAGACTTCACACCTAACAACCACGGTCTGGTAGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTCGCGCGACGGCGGCGTCGCACCATGTCACGCCGActtataaca                                                                                >  2:965676/1‑137 (MQ=255)
                                                                   gTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTCAGTAACCcg                                                                           >  1:731358/1‑143 (MQ=255)
                                                                        ccGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGCTTTTAAATAACCCggcggc                                                                      >  2:235959/1‑143 (MQ=255)
                                                                                cacaCCTAACACACACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTc                                                              <  1:1116799/143‑1 (MQ=255)
                                                                                cacaCCTAACAACCACGGTCGGGTGGTGCGCGGATCTAAACGCATCCCCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATTTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTc                                                              <  1:837027/143‑1 (MQ=255)
                                                                                   acCTAACAACCACCGTCTGGTGGTGCGCGGATCTAAGCGCATCGCCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTa                                                           <  1:346721/143‑1 (MQ=255)
                                                                                       aacagcCACGCGCTGGTGGTGCGCGGATCTAAACGCATCACCCCTGGGTAAAACCTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAacac                                                       <  1:313085/143‑1 (MQ=255)
                                                                                        acaacCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCTGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACg                                                      >  1:1126161/1‑143 (MQ=255)
                                                                                          aacCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGCAGAATGTCATGGCGATTTTAACTAACCCGGCGGCAGCGGGTACTAacagcat                                                    >  1:1047680/1‑139 (MQ=255)
                                                                                             cACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATccc                                                 >  2:137686/1‑143 (MQ=255)
                                                                                              gcGGTCTGGTGGTGCGCGGATCTAAACGCTTCCGCACAGTGGAAAAACTTTTACCCAGCAAACCCGCTGGCGTGCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATcccc                                                <  2:494545/142‑1 (MQ=255)
                                                                                               cGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCcca                                               <  1:898598/143‑1 (MQ=255)
                                                                                               cGGTCCGGTGGTGCGCGGCTCTAACCGCACCACCTCTGGATAAAGACTTTTACCCAGCCACCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCcca                                               <  2:747461/143‑1 (MQ=255)
                                                                                                            cgcgGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGtgct                                  >  1:330563/1‑143 (MQ=255)
                                                                                                               ggACCTAAACGCATCGCCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATACCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCa                               <  2:645772/143‑1 (MQ=255)
                                                                                                                gATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCCGGTCCTAACACGATCCCCACCAACCCCGCGCTCCAc                              >  2:998770/1‑143 (MQ=255)
                                                                                                                 aTCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCCGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCCGCAGCCGGACCCCACACGCTCCCCACCAACACCATGCTGCAcc                             >  2:1131169/1‑142 (MQ=255)
                                                                                                                  tCTAAACGCAGCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGa                            <  1:106575/143‑1 (MQ=255)
                                                                                                                   cTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACgcg                           >  2:302617/1‑141 (MQ=255)
                                                                                                                           aTCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGTGTTACtcgc                   >  1:104043/1‑143 (MQ=255)
                                                                                                                               ccTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGAc               <  1:137686/143‑1 (MQ=255)
                                                                                                                                ctctGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGAcc              >  1:1099775/1‑143 (MQ=255)
                                                                                                                                   tGGGTAAAAGTTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGa           >  2:87907/1‑143 (MQ=255)
                                                                                                                                    gggTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGAt          <  2:330563/143‑1 (MQ=255)
                                                                                                                                     ggTAAAAGTTTTTACCCAGCAAACCCGCTGGTGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCGGAACACCATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATg         >  1:1123205/1‑143 (MQ=255)
                                                                                                                                         aaaaCTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGt     >  1:901712/1‑143 (MQ=255)
                                                                                                                                            aCTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGCGTggg  >  1:752856/1‑143 (MQ=255)
                                                                                                                                             |                                                                                                                                             
AGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGG  >  NC_000913/3131384‑3131666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑