Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 187,458 C→T 10.0% R398H (CGC→CAC)  glnD ← PII uridylyltransferase/uridylyl removing enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913187,4580CT10.0% 115.6 / 2.7 51R398H (CGC→CAC) glnDPII uridylyltransferase/uridylyl removing enzyme
Reads supporting (aligned to +/- strand):  ref base C (23/20);  new base T (2/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTTCATCACGCAGGTCGATTAGCGTACCGCGTAGCTGAAACTC  >  NC_000913/187325‑187594
                                                                                                                                     |                                                                                                                                        
gCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCGAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGCCGACGGGCATGGCGTAACTGGCGCAGAgtgg                                                                                                                                 >  2:177744/1‑143 (MQ=255)
gCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCgtgg                                                                                                                                 >  1:9047/1‑143 (MQ=255)
           gCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGGaaacg                                                                                                                      >  2:1053578/1‑141 (MQ=255)
            ctttGGCAATACACCGCGGCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTCGAGTAAATGc                                                                                                                     <  2:558025/141‑1 (MQ=255)
            cATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAGCTGGCGCAGCGTGGTGGAGTAGATGc                                                                                                                     >  1:534775/1‑143 (MQ=255)
            cATTGGCAATAGCCCGCGCCGCACCGCGCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGc                                                                                                                     <  2:250692/143‑1 (MQ=255)
                cgCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTCTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACCGGCATGGCGTAACTGGCGCAGCGCGGCGGAGCCAATGCCggc                                                                                                                 >  2:460871/2‑142 (MQ=255)
                  ccatcgCCCGCGACGCACCGCTCCGGTGGGACGCAGAATGCTCAAAACCAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGa                                                                                                               <  2:904271/138‑1 (MQ=255)
                  cAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGTGCAGCGTGGTGGAGTAAATACCGGCGa                                                                                                               >  2:989192/1‑143 (MQ=255)
                  cAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAACACCACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGTGCAGCGTGGTGGAGTAAATGCCGGTGa                                                                                                               >  2:986346/1‑143 (MQ=255)
                       gCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTACGTGCCGCCGATCGCa                                                                                                          >  1:1111264/1‑143 (MQ=255)
                           gcgcCGGACCGCGCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTGCCGGAATATTACACAGCGGTGGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACtgt                                                                                                      <  2:126998/143‑1 (MQ=255)
                           gcgcCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCAGGAAGACGACGCGCCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGCGGTGGCGTAAATGCCGGTGATCGCACtgt                                                                                                      >  2:501568/1‑143 (MQ=255)
                              ccgcACCGCTCCGTGGTGACGCATAATTCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCTGATGGCGATGGGCATGGCGTAACTTGTGCAGCGTTGTGGAGTAAATGCCGGTGATCGCACtgttgt                                                                                                   <  2:401349/143‑1 (MQ=255)
                              ccgcACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACtgttgt                                                                                                   <  2:888325/143‑1 (MQ=255)
                               cgcACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGCTGATGTAGCAGCGTGGTGGAGTAGATGCCGGTGATCGCACTGTtgtg                                                                                                  >  1:93992/1‑143 (MQ=255)
                                    cGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCCCACGGTTGTGCACCa                                                                                             >  2:841773/1‑143 (MQ=255)
                                        gcgcggTGACGCAGACTGCTCAACACCCGTTTTCGTGCTTGGGGAAGATTACCCAGCGGTTGTTGCAGCGGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGgt                                                                                         <  2:340169/139‑1 (MQ=255)
                                        ccGGGGTGACGCAGAATGCTCAAACAAAGTCGTCGTGCGTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGgt                                                                                         <  1:764855/143‑1 (MQ=255)
                                         cGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGgtg                                                                                        <  2:371186/143‑1 (MQ=255)
                                             gTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGa                                                                                    >  1:762234/1‑143 (MQ=255)
                                                  gCAGAATGCTCAAGAATAGTTTTCGTGCTTGCGGAATATTACACGGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATa                                                                               <  2:86895/143‑1 (MQ=255)
                                                   cAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGACTATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGACCATAc                                                                              >  2:770437/1‑143 (MQ=255)
                                                        tGCTCCAAACCAGTTTTCGTCCCTCCGTAATATTACACCCCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTTTAGAACATACGCCAg                                                                         <  2:954508/143‑1 (MQ=255)
                                                           tCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATTGCGCCGGGCATGGAGTAACTGGCGCAGCGTGGTGGAGTAAAGGCAGGTGATAGCACTGAGCGGGATCCTGGTGTAGAACACACGCAAGATg                                                                      >  2:278611/1‑143 (MQ=255)
                                                           tCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATg                                                                      >  1:909561/1‑143 (MQ=255)
                                                           tCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCAGTGCGTAGCTGTGACTACGAGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGAAAGATg                                                                      >  1:523609/1‑143 (MQ=255)
                                                             aaaaaCAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACACACGCAAGATCGc                                                                    >  2:151871/1‑143 (MQ=255)
                                                              agagcAGTTTTCGTGCTGCCGGAATAGTACACAGCGGTTGGTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCt                                                                   <  2:259033/139‑1 (MQ=255)
                                                                aaCAGTTTTCGGGCTCCCGGAATATTACACAGCGGGTGGTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTc                                                                 <  1:1058348/143‑1 (MQ=255)
                                                                 aCAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGGTTcc                                                                >  2:147518/1‑143 (MQ=255)
                                                                      tttCGTGCTTCGGGAATGTTACCCAGCGGGTGTTGCAGCTGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTg                                                           <  2:162946/143‑1 (MQ=255)
                                                                      tttCGTGCTTCCGGAATATTACGCAGCGGTTGTTGCAGATGGCGCCGGGCATGGCGTAACTGGCGCAGCGTGGTGTAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGATCATACGCAAGATGGCTTCCGGCTg                                                           <  2:640929/143‑1 (MQ=255)
                                                                        tCGTGCCTGCGGGATATGGCACAGCGGTTGTTGCAGAGGGCGACGGGCAGGGCGTAACTGGCGCAGCGTGGTGGAGGAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGgc                                                         <  2:971271/143‑1 (MQ=255)
                                                                         cGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGgcg                                                        <  2:9047/143‑1 (MQ=255)
                                                                               tCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATaaa                                                  <  1:278855/143‑1 (MQ=255)
                                                                                      tatTACACAGTGATTGTTGCAGATGGTGACGGGCATTGTGTAACTGTCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGtt                                           <  2:225536/143‑1 (MQ=255)
                                                                                        ttACACAGCGGTTGTTGCCGATGGCGACGGGCATGGCGTTACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATAGCACTGTTGTGCACCATGGTGTAGAAAATACGAAAGATGGCTTCCGGCTGGCGAATAAAGAGTGTTtc                                         >  1:146221/1‑143 (MQ=255)
                                                                                          acacAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGTGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCAT‑GTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTtcatc                                      <  1:986346/143‑1 (MQ=255)
                                                                                          acacAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGAGGGCTTCCGGCTGGCGCAGAAATAgtgtcacat                                       >  2:340643/1‑138 (MQ=255)
                                                                                           cacaGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGTGCAGCGTGGTGGAGTAAATACCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTtcatc                                      <  1:989192/143‑1 (MQ=255)
                                                                                               gCGGGTGTTGCAGATGGCGACGGGCATGGCGTAACTGGGGCAGCGTTGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTTCATCACGc                                  <  1:1114665/143‑1 (MQ=255)
                                                                                                cGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTTCATCACGCa                                 >  1:680620/1‑143 (MQ=255)
                                                                                                      ttgCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTTCATCACGCAGGTCGa                           >  1:264089/1‑143 (MQ=255)
                                                                                                        gCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTTCATCACGCAGGTCGAtt                         <  1:1000046/143‑1 (MQ=255)
                                                                                                          aGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCAGAAATAGTGTTTCATCACGCAGGTCGATTAg                       >  2:45787/1‑143 (MQ=255)
                                                                                                             tGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGGGTTTCATCACACAGGTCGATTAACGt                    >  2:414386/1‑143 (MQ=255)
                                                                                                                    gggCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTTCATCACGCAGGTCGAGTAGCGTACcgcgg             >  1:191831/1‑142 (MQ=255)
                                                                                                                    gggCATGGCGTAACTGGCGCAGCGTGGAGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGATTCATCACGCAGGTCGATGAGCGTACcgcat             >  2:256761/1‑141 (MQ=255)
                                                                                                                           gCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTTCATCACGCAGGTCGATTAGCGTACCGCGTAGCGGaa      >  1:1111683/1‑143 (MQ=255)
                                                                                                                               aaCGGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCTTCTTGTTGTATAATATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTTCATCACGCAGGTCGATTAGCGTACCGCGTAGCTGAAACTc  <  1:177744/143‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
GCTATGGCGATGCATTGGCAATAGCCCGCGCCGCACCGCTCCGGGGTGACGCAGAATGCTCAAAAACAGTTTTCGTGCTTCCGGAATATTACACAGCGGTTGTTGCAGATGGCGACGGGCATGGCGTAACTGGCGCAGCGTGGTGGAGTAAATGCCGGTGATCGCACTGTTGTGCACCATGGTGTAGAACATACGCAAGATGGCTTCCGGCTGGCGCATAAATAGTGTTTCATCACGCAGGTCGATTAGCGTACCGCGTAGCTGAAACTC  >  NC_000913/187325‑187594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑