Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,286,116 C→G 7.0% G419A (GGC→GCC)  acs ← acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,286,1160CG 7.0% 142.4 / 2.7 50G419A (GGC→GCC) acsacetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base C (22/24);  new base G (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

AGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGAT  >  NC_000913/4285981‑4286258
                                                                                                                                       |                                                                                                                                              
aGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGCTTGCACGCCGAAGAACGGACGTGTTGCCGCACCGGCTTTCAGCTCGGTAGCGCCAGGCCGCGGGGTGCCCACGGAACCGCCGGGTTc                                                                                                                                         >  2:638460/1‑143 (MQ=255)
aGGCTACCTTCGGTGGCACCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAAAACGAACGTGTTGCCGAACCGGCTTTAAGCTAGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTc                                                                                                                                         >  1:999203/1‑143 (MQ=255)
 gccTACCTTCGGTGGCCCCCTCCCGTGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCg                                                                                                                                        <  2:387921/141‑1 (MQ=255)
  gCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGGGTTCCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGCGGTCATGAAACCGCCgggggcgg                                                                                                                                       >  2:881165/1‑137 (MQ=255)
   cTAGCTTCGGGGGCGCCCTCCAGCGGGTTACCTGCGTTGTCGACCAGCGCCGGATGCACGCCGAAGATCGGTTTTGTCGCCTAACCTGCTTTCAGCTCGTTAGCGCCAGGCAGCGGGGTGATCATGAAATCGCCGGTTTCGGt                                                                                                                                      <  2:610914/143‑1 (MQ=255)
         tCGGTGGCCCCCTCCGGCGGGTTAGCTTCGTTATCGCCCAGCTCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGcca                                                                                                                                <  2:1065836/143‑1 (MQ=255)
              gggtcccTCCAGCGGGTCACCTTCGTTATCGGCCAGCGCCGGTGGCGCGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAgg                                                                                                                           <  1:891694/139‑1 (MQ=255)
                 ccccTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGAGCATGAAACCGCCGGGTTCGGTCTGCCACCAgcaat                                                                                                                        >  1:873306/1‑139 (MQ=255)
                  cccTCCAGCGGGTGACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATc                                                                                                                       <  2:750104/143‑1 (MQ=255)
                         gCGGGGTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGaccacc                                                                                                                <  1:753111/143‑1 (MQ=255)
                             gTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGAc                                                                                                            >  1:716987/1‑143 (MQ=255)
                              gtagcTTCGTTATCGACCAGCGCCGGTTGCACGGAGACAGGCGGAGGGTTCCCCGAACCGGCTTTCAGCGCGGTAGCGCCATGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACa                                                                                                           <  2:797137/139‑1 (MQ=255)
                                  cTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGACCCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGGGGGCATGAAACCGCCCGTTTCCCTCCGCCACCAGGAACCGACCACCgggcgtcgc                                                                                                       >  2:880985/1‑136 (MQ=255)
                                    tCGTTATCCACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTtctc                                                                                                     <  1:457572/143‑1 (MQ=255)
                                         ctcagtcAGCGCCGGTTGCACGCCGAAGACCGGACGGGTTGCCGAACCGGCGTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGc                                                                                                <  2:1031314/137‑1 (MQ=255)
                                            gACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCGCGGTAGCGCCAGGCAGCGGGGTGATCATGCAACCGCCGGTTTCGGTCTGCCACCAGGCCACGACCACCGGAGATTCGTCGTGGCCGa                                                                                             >  2:478511/1‑143 (MQ=255)
                                               cAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAttt                                                                                          >  1:984561/1‑143 (MQ=255)
                                                    ccGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTCCTCGTAGCCGCTTGTTTTc                                                                                     >  2:261331/1‑143 (MQ=255)
                                                    ccGGCTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTc                                                                                     <  1:707936/143‑1 (MQ=255)
                                                    acGGTTGCGCGCCGAAGAACGGACGTGTGGCCGAACCGGCTGTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTc                                                                                     <  2:213290/142‑1 (MQ=255)
                                                      ggTGGCACGCGGAAGAGCGGACGGGTTGCCGAACGGGCTTTCAGCTGGGTAGCGCCAGGCAGCGGGGTGGTCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCa                                                                                   <  2:879339/143‑1 (MQ=255)
                                                          gCACGCCGAAGAACGGACGGGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTAc                                                                               <  1:1003884/143‑1 (MQ=255)
                                                           cACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTAcc                                                                              >  1:412849/1‑143 (MQ=255)
                                                           cACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTCTCGGTCTGCCACCAGGGATCGACCACCGGACAGTTCTCGCTGCCGGTGGTTTTCCAGTGcc                                                                              >  2:293408/1‑143 (MQ=255)
                                                                cGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGCTTTTTTTCCAGTACCACTcc                                                                         >  1:359017/1‑143 (MQ=255)
                                                                  aagaaCGGACGTGTTGCCGAACCGGCTTGCAGCGCGATAGCGCCGGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCa                                                                       <  1:490045/143‑1 (MQ=255)
                                                                    gaaCGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTCGCCGATATTTTTCCAGTACCACTcacccg                                                                     >  2:1071429/1‑138 (MQ=255)
                                                                         gACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTcc                                                                <  1:725993/143‑1 (MQ=255)
                                                                          aCGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGTTTCCg                                                               >  1:643142/1‑143 (MQ=255)
                                                                             tgtTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACCTTTCTCGTTGCCGATCTTTTTCCAATCCTACTCCCAAGCTTCCGGGt                                                            >  1:700267/1‑143 (MQ=255)
                                                                               ttGCCGCAGCGGCTTCCAGCGCGGTAGCGCCGGGCAGCGGGGTGATCATGACACCGCCGGTTTCGGTCTGCCACCTGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTa                                                          <  2:360622/143‑1 (MQ=255)
                                                                                 gCCGAAGCGGCGTTCGGCTCGGTAGCGCCAGGCAGGTGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGCTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAAt                                                        <  2:851147/143‑1 (MQ=255)
                                                                                 gCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGACACCGCCGGTTTCGGTCTGCCACCGGGTATCGACCACCGGACCTTTCTCGCTGGCGCTTTTTTGCCAGGACCGCCCCCGCGCTCCCgggctcgc                                                        >  2:976036/1‑138 (MQ=255)
                                                                                 gCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAAt                                                        <  1:741907/143‑1 (MQ=255)
                                                                                 gCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCACGTATCGACCACCGGACATTTCTCGATCCCGATGTCTTTCCACCAACGCCCCCACCCTACCGGGGTagt                                                        >  2:373127/1‑141 (MQ=255)
                                                                                      aCCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCt                                                   >  1:291173/1‑143 (MQ=255)
                                                                                       ccGGCTTTCAGCGCGGTTGCGCCAGGCAGCGGGGTGATCACGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTc                                                  <  1:1120653/143‑1 (MQ=255)
                                                                                            tttCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGCTAATTGGCCCGgccc                                             >  1:951175/1‑142 (MQ=255)
                                                                                                aGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTACTTGGCTCGCCCCCGGa                                         >  1:1036631/1‑143 (MQ=255)
                                                                                                      gTAGCGCCAGGCGGCGGGGTGATCGTGAGACCGGCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTGCTCGTTGCCGATTTTTTTCCAGTACCACGCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCgag                                   <  2:221632/143‑1 (MQ=255)
                                                                                                        tgcgcCTGGCAGCGGGCTGGTCATGAAAGCGCCGGTTTCGGTCGGCGACCAGGTGTCGACCACCGGACATTGCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGaa                                 <  2:294710/142‑1 (MQ=255)
                                                                                                        ggcgcCAGGCGGCGCGGTGAGCATGCGGCCTCCGGTTTCGGTGTGCCACCAGGTATCGCCCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCCCGCCCACGGAACCGAGaa                                 <  2:364434/142‑1 (MQ=255)
                                                                                                        aGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGaa                                 <  1:1044686/143‑1 (MQ=255)
                                                                                                             cAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCCCGGAACCGAGAATgcgc                            >  1:709285/1‑143 (MQ=255)
                                                                                                              aGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCa                           <  1:526455/143‑1 (MQ=255)
                                                                                                                     ggggTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGcgacga                    >  1:933432/1‑143 (MQ=255)
                                                                                                                            gaccgAAACCGGCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAGTTGGCTCGCCCCCGGAACCGAGAATGCGCAGCGACGAAcggtcg             >  1:666683/5‑143 (MQ=255)
                                                                                                                              ccgAAACCGGCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGc                                                    <  1:608283/100‑1 (MQ=255)
                                                                                                                              ccgAAACCGGCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGc                                                    >  2:608283/3‑102 (MQ=255)
                                                                                                                                gAACCCGCCGGTTTCGCTCCGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGc         <  1:446984/143‑1 (MQ=255)
                                                                                                                                    cctccgGTTTCCGTCTGCCACCAGCTATCGCCCCCGAGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTc     <  2:999203/140‑1 (MQ=255)
                                                                                                                                     cgccgGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGACTGCGCAGCGACGAACGGTCGGTGCCTTCg    >  1:550512/1‑143 (MQ=255)
                                                                                                                                      gccgGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAAACGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGa   >  1:601135/1‑143 (MQ=255)
                                                                                                                                       ccgGTTTCGGTTTGCCACCAGGTATCGACCACCGGACATTTCTCGTGGCCGATTTTTTTACAGTACCACTCCCGCGCTCGCGGGGTGGTTGGCTGGCCGCCGGTACCGCGGATGCGCGGGGGCGCACGGCGGCGGCCTTCggg  >  2:814035/1‑141 (MQ=255)
                                                                                                                                       ccgGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGCTCTTTTTCCAGTACCACTCCCACGCTACCCGGTTCATTGGCTCCCCCCCGCTACCGCCAATGCCCAGCCACGACCGCTCCGCGCCTCCgcc  >  2:1022778/1‑141 (MQ=255)
                                                                                                                                       ccgGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGCGAATGCGCAGCGACGAACGGTCGGTGCCTTCgcc  >  1:141105/1‑141 (MQ=255)
                                                                                                                                       |                                                                                                                                              
AGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGAT  >  NC_000913/4285981‑4286258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑