| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,472,434 | G→A | 7.6% | R34* (CGA→TGA) | pyrL ← | pyrBI operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,472,434 | 0 | G | A | 7.6% | 158.7 / 3.1 | 61 | R34* (CGA→TGA) | pyrL | pyrBI operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base G (27/29); new base A (2/3); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01 | |||||||||||
TGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCA > NC_000913/4472297‑4472574 | tGCTTTCATTTTCGCCGCTGTCGCCATCACCAGATTAAGGTCATCGCTACTAATGTCGTTTATGGAAATGATATGTTTCTGAGATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGa < 1:434765/143‑1 (MQ=255) gCTTTCAGTGTCGCCGCTATCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAg < 1:942172/143‑1 (MQ=255) tCAGTTTCGCCGCGGTCGCCAGCACCAGATTAAGGTGATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGc < 1:81902/143‑1 (MQ=255) tCAGTTTCGCCGATGTCGCCAGCGACAGATTAAGGTCACCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGc < 1:284449/143‑1 (MQ=255) cAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCt < 1:867096/143‑1 (MQ=255) aGTTTCGCCGCTGTCGCCAGCACCAGCTTCTGGTCATCGCGACTAAGGTCGTATATAGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTg < 2:737706/143‑1 (MQ=255) aGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTg < 2:747346/143‑1 (MQ=255) gTTTGGCCGCTGGCGGCAGCTCCAGATTAAGGTCATGGCGCCGACGGTCGTTTATGGAAATGATATGTCTCTGAAATATCGGATTAGCCATCTTTTACCACCTGTCTCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTgg < 2:190957/143‑1 (MQ=255) gTTTCGCCGCTGTCGCCAGCATCAGATTAAGGTCATCGCGGCTAATGTCGTTTATGGAAATGATATGTTTCTGAGATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCttt < 1:635570/143‑3 (MQ=255) gTTTCGCAGCTGTCGCCAGTACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCAATCGAGGGGCttt < 1:620078/143‑3 (MQ=255) tgtccgcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGaa < 1:689432/140‑1 (MQ=255) ccgcTGTCGTCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTCTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATgg < 1:879597/143‑1 (MQ=255) gcTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAAAGGGt > 2:157141/1‑143 (MQ=255) tGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGa < 1:193514/143‑1 (MQ=255) gTCGGCAGCAACAGAGTCAGTTCACCGCGAATAAGGTCGTTGATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATTTTTTAGCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAt < 2:302870/143‑1 (MQ=255) gTCGCCCGCACCAGATTCAGGTCATTGCGACTAAGGTCGTTTATGTAAATGTTATGTGTCTAATAGAGCTGATGAGCCATATTTAATCTTCTGACCCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAt < 2:452742/143‑1 (MQ=255) aGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACgg > 2:889992/1‑143 (MQ=255) cAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCATGGGCAAAAAAAAGACCCTAGATTAAGGGGCTGGGAATGGGAGAT‑CCACGGgaaaa > 2:493166/1‑141 (MQ=255) gTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATGGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAACAACGGCa > 1:449193/1‑143 (MQ=255) tCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGCAGAAAAACGGCAg > 1:741114/1‑143 (MQ=255) tCGCGACTAAGGTCGTCTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGGAAAACGGCAGGcc > 1:885235/1‑143 (MQ=255) aGGTCGTTTGTGGAGATGATGTGTTTGTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGGCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCttt < 2:168670/143‑1 (MQ=255) tCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGCATGGGTGAT‑CAACGGGTAGAAAATCGGCAGGCCAGCGTCtttttt < 1:889272/143‑1 (MQ=255) ccGTTGATGGAAATGATATGTTTCTGAAAAAATATATTTGCTATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCtttttt < 1:765021/142‑1 (MQ=255) tttATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAg > 2:536311/1‑143 (MQ=255) aaTGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTa > 1:300495/1‑143 (MQ=255) caatatGTTTTTTATATATCGGATTATTTATTTTTTATCACGAGAGGGCGGGGCAAAAAAAGGGCCCTCGATGGGGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGTGTCTTTTTTCAGACGCGGTAAg < 1:786963/141‑1 (MQ=255) atGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACaa > 2:890405/1‑143 (MQ=255) gTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCGGCGTCTTTTTTCAGACGCGGTAAGACaaaa > 1:366404/1‑143 (MQ=255) tCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGACTGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATgc > 2:71796/1‑142 (MQ=255) cTGATATAGCGGATTAGGCATCTTTTATCTCGTGACGCCTGGGCAAAAAAAAGCCCCACGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc < 1:947978/143‑1 (MQ=255) cTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc < 1:889992/143‑1 (MQ=255) cTGATATAGCGGATTAGCCAGCTTTTATCGCCTGACGCCCGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTc < 1:842096/143‑1 (MQ=255) tgctataGCGGATTAGCCCTCTTTTATCTCCTTACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg < 2:419502/140‑1 (MQ=255) tGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg > 2:701363/1‑143 (MQ=255) tGATATAGCGGATTAGCCATCTTTTACCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATTGGTGCT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCg < 1:747431/143‑1 (MQ=255) gATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGCATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCgc > 2:929378/1‑142 (MQ=255) atagAGCGGATTAGCCATCTTTTATGTCCTGACGCCGGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGaa < 1:778501/143‑1 (MQ=255) ataGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAaca < 1:911407/143‑1 (MQ=255) aGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAacacc > 1:861736/1‑142 (MQ=255) gCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGGAAGACAAAATGTCGAacacac > 2:34418/1‑143 (MQ=255) aTTAGTCATCTTCGATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGGATGGGTGAT‑CAACGGTAAGAAAACCGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGaa < 2:745894/143‑1 (MQ=255) ttAGCCATGTTTTATGACCTGACGGCTAGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAGCGGCCGGCATCCCTCTCCTGGCAGACGCGGTAAGACAAAATGTCGAACACACTGAAc < 2:242462/143‑1 (MQ=255) gCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCAt > 2:619501/1‑143 (MQ=255) gCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATCGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCAt < 1:116393/143‑1 (MQ=255) ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtcc > 1:803357/1‑143 (MQ=255) ttttATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACAtcc > 1:177958/1‑143 (MQ=255) aTCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTccc > 1:234851/1‑143 (MQ=255) accccTGACGCCTGCGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCg < 1:938548/140‑1 (MQ=255) tcCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACGAAATGTCGAACACACTGAACCATACCTCCTCCCGGc > 2:822178/1‑143 (MQ=255) tGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGTCAGCGTCTTTTTTCAGACGCGGTAAGACAAAAGGTCGAAGACACTGAACCATACATCCTCCCGGCaaa < 1:719470/143‑1 (MQ=255) cTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTC‑TTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCg > 2:616350/1‑143 (MQ=255) tGGGCAAAAAAAAGCCCCTCGATCGAGGGGCTGGGCA‑CCCTGATACAACGGTAAGAAAAACGGCAGGCCAGCGTCTTGTTACAGACGCGGTAAGAAGAAAGGTCGAACATACTCAATCGTACTTCGTCCCTGCAAAttttgga > 2:1107506/1‑138 (MQ=255) ggCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGc > 1:916968/1‑143 (MQ=255) ggCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGc > 2:252790/1‑143 (MQ=255) cAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCCTACATCCTCCCGGCAAATTTTCCGGCAt > 1:985221/1‑143 (MQ=255) ccaGCCCCTCAATCGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGCCGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCGTTATACt > 1:114708/3‑143 (MQ=255) caGCCCCTCAATCGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTc > 2:526688/2‑143 (MQ=255) ccTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGt < 1:701363/143‑1 (MQ=255) cTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTc > 1:319067/1‑143 (MQ=255) tCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGACCGTATACTCATCGCCa > 2:367949/1‑143 (MQ=255) | TGCTTTCAGTTTCGCCGCTGTCGCCAGCACCAGATTAAGGTCATCGCGACTAAGGTCGTTTATGGAAATGATATGTTTCTGATATAGCGGATTAGCCATCTTTTATCTCCTGACGCCTGGGCAAAAAAAAGCCCCTCGATTGAGGGGCTGGGAATGGGTGAT‑CAACGGGAAGAAAAACGGCAGGCCAGCGTCTTTTTTCAGACGCGGTAAGACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCA > NC_000913/4472297‑4472574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |