| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,479,020 | A→T | 6.6% | intergenic (+131/+14) | rraB → / ← yjgM | ribonuclease E inhibitor protein B/putative acetyltransferase YjgM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,479,020 | 0 | A | T | 6.6% | 156.9 / 2.2 | 59 | intergenic (+131/+14) | rraB/yjgM | ribonuclease E inhibitor protein B/putative acetyltransferase YjgM |
| Reads supporting (aligned to +/- strand): ref base A (25/27); new base T (2/2); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
GCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAG > NC_000913/4478882‑4479162 | gCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGGATGCTCGCGAGTTGTAcgcgcg > 1:395609/1‑143 (MQ=255) ccACTAGTTAAGCGTAGCGCATCAGGCCCAGGTAGTGCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATACACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCgg < 2:413151/143‑1 (MQ=255) cgcTAATCCAGCGTAGCGCATCAGGCCCAGGTTGTTCCTGCATCCTGGTGAATTTGTAGCCCGCATAAGGCATTCATGTCACCTCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGTGTTCAACTCGCggg < 2:877433/141‑1 (MQ=255) cACTTACTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCggg < 2:1072807/143‑1 (MQ=255) ttAAGCGTAGCGTATCAGGCCTAGGTAGTGCCTGCAAGCTGGCGAATTTGTAGCCCGGATAAGGCATTCATGCCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAtttt < 1:223029/143‑1 (MQ=255) ttAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAAGAAAACGTACGTTGTTAGTGTTCGACTGCCCGCGGGCTGAACCCGCGGGCGtttt > 1:192735/1‑143 (MQ=255) ttAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCAtttt > 1:138471/1‑143 (MQ=255) tAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGCTAAGGCATCCCTGTCACATCCGGCATAAACAACACGTACGTTGTTAATGTTCGAATACCCGCGTGCTGAACAGGCGGCGAttttt > 1:863534/1‑143 (MQ=255) gTAGCGCATCAGGCCCAGGTAGTTCCTGCAAGCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTaga < 2:866975/143‑1 (MQ=255) gcgcATGACGCCCAGTGAGTTCGTGCCACCTGGGGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGtt < 2:819919/143‑1 (MQ=255) gcgcATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGACAAGGCAGTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGtt < 2:979017/143‑1 (MQ=255) cATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTcgc > 1:720169/1‑143 (MQ=255) tCAGGCCTAGGTCGTTCCTGCAACCTGGTGAATTTGGAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCgcgc < 2:313659/143‑1 (MQ=255) cAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGGTGAACACGCGCGCAGTTTTTTAGAGGTCGCGCa > 2:6524/1‑143 (MQ=255) cAGGCCCAGGTAGTTCCTGCAACCAGGTGGATTTTTAGCCCGGATAAGGGATTCAAACAACAGCCGGCATCATCACATCTCGCGTTGTTGATGTTCGAATGCCCGCGAGTTTAACACGCGGGCATTCTTGTAGAGCTCGCGCa > 2:1103744/1‑143 (MQ=255) ggCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGGATTCATGTCACATCCGGCATAAACAAATCGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAAc < 2:566915/143‑1 (MQ=255) cccAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACAt > 1:760726/1‑143 (MQ=255) aGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCAGATCCAGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACACCCg > 2:152274/1‑143 (MQ=255) aGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCg > 1:894725/1‑143 (MQ=255) aGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTCTAGAGTTCGCACAACATCCg > 2:637639/1‑143 (MQ=255) gTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCa < 1:219971/143‑1 (MQ=255) gTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTGGGTAATGGTCGAATGCCCGCGAGTTGAGCACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCa > 1:778035/1‑143 (MQ=255) gTAGTTCCTGCAACCGGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCa < 1:338920/143‑1 (MQ=255) tAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCAGcgc < 2:196447/143‑3 (MQ=255) ttCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTc > 1:538675/1‑143 (MQ=255) tacatcatGGTGAAGTTGTAGCCCGGATACGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTGAATTTCGCTCAACATCCGCACTTCACaa < 2:403318/136‑1 (MQ=255) tGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACaa > 1:974382/1‑143 (MQ=255) cccTGGTGGATTTGGAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGa < 2:448827/142‑1 (MQ=255) ttGTAGCGTGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTg < 2:102839/143‑1 (MQ=255) cgtaccgcGGATAGGCCAGTCATGTCCCATCCTGCATAAAAAATCCGTACGTTGTTAATGTTCCAATGCCCGCGAGTTTAACACGCGGGCATTGTTTTAGAGTCCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGc < 2:107562/136‑1 (MQ=255) tAGCCCGGATGAGGCAGCCGAGTCAGATCCGGCATACGCAAAACGTACGTTGTTAATTGTCGAATGCGCGCGAGTTGACCACGCGGGCATTTTTTTAGATTTTGCGCAACTTCTGAACTTCACTATCGTCATGGCCCGTGCAg < 2:880336/143‑1 (MQ=255) ggATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGCTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCCTTTTTTTAGAGTTCTCGCAACATCAGCCCTGCGCAATCGACATAGCCCGTGCAGACaacg > 2:182426/1‑141 (MQ=255) ggAGAACGCATTCATGTCACATCCGGCATAACCAAAAGGAACGTACCAAAAATAGGTATGCCCACGAGTTCAGCACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAgc < 1:474394/143‑1 (MQ=255) taTAAGGCAGTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAgcg < 1:166562/142‑1 (MQ=255) gCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGt > 1:868068/1‑143 (MQ=255) aTTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCg > 2:497614/1‑143 (MQ=255) ttCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCAGCGTTTTACGACCGACATGGCCCGTGCAGCCAAGCGCATAGTCGa > 2:796262/1‑143 (MQ=255) gTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGc < 1:840919/143‑1 (MQ=255) gccGGCAGAAAGAAAACGGACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAg < 2:325180/142‑1 (MQ=255) gccGGAAGAAACAAAACGTACGTGGTTAAGGTGCGAAGGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAg < 1:122873/142‑1 (MQ=255) ccGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGc < 2:894725/143‑1 (MQ=255) ggCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGccc > 1:433498/1‑143 (MQ=255) aaCAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAACCCAAATGc > 2:31209/1‑143 (MQ=255) aCAAAACGTACGTTGTTAATGTTCGAATGCCCGAGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCt > 2:1107867/1‑143 (MQ=255) cAAAACGTACGTTGTTAGTGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTc < 1:605302/143‑1 (MQ=255) aaCGTACGTTGTTAATGTTCGAATGCCAGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATa < 2:240990/143‑1 (MQ=255) cctacgtTGTTATTGTTCGATTGTCCGCGAGTTGATCTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATaaa < 1:199693/141‑1 (MQ=255) tcgttAATGTTCGCATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCaa < 1:229729/141‑1 (MQ=255) cgcaTGCCCGCGAGTTGAACCCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTACCttt < 2:460784/140‑1 (MQ=255) cccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTaaaaaa < 1:497614/143‑1 (MQ=255) ccGCGTGTTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAg > 1:310394/1‑143 (MQ=255) ccGCGTGTTCAACTCGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAg > 1:966761/1‑143 (MQ=255) ccGCGAGTTTAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAACGCGCCATGGCCTCCTTTCAAAAAg > 2:1055579/1‑143 (MQ=255) ccGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCCGCCAAGCCCCTAGTCGATAAGCTCAAAGCCCAAATGCTCGTAAAGCGCAATGGCTTCCTCTaacggag > 2:839395/1‑138 (MQ=255) gcgAGTTGAACGCCCGGGCACTGTTGTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGGGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATAGCTTCCTTTAAAAAAGCg < 2:192735/143‑1 (MQ=255) cgAGTTGAACACGCGTGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCgg < 2:760726/143‑1 (MQ=255) cgAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCGCAATGGAGATGGCCAGTGCGGCCAAGCGCATAGTCGGTAGGCTCAAAGCCCAAATGCTCATAAAGCGGAGTTGCCTCCGTTAAAAGAGCgg > 2:835182/1‑143 (MQ=255) ttGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGGCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGt > 1:1051588/1‑143 (MQ=255) tGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTGACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTAAAAGCCCCAATGCTCATCAAGCGCAATGGCTTCCTTTAGAAAAGCgtgtgtt > 2:325229/1‑137 (MQ=255) gAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCTACATGGCCCGTGCAGCCAAGCGCATATTCGATATTCTCAACGCGCAAATGCTCCTCAAGCGCAATTGCTTCCTTTAAAGCAGCGGTCGttt > 2:724436/1‑143 (MQ=255) acacGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGAATTCATTTAAAAAAGCGGACGattaa > 1:1109122/1‑141 (MQ=255) acGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACCTGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAACGGCTTCCTTTAAAAAAGCGGTCGATTcccg > 2:919605/1‑141 (MQ=255) acGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTGGTTTCCAg > 1:377221/1‑143 (MQ=255) | GCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAATTTGTAGCCCGGATAAGGCATTCATGTCACATCCGGCATAAACAAAACGTACGTTGTTAATGTTCGAATGCCCGCGAGTTGAACACGCGGGCATTTTTTTAGAGTTCGCGCAACATCCGCACTTCACAATCGACATGGCCCGTGCAGCCAAGCGCATAGTCGATATGCTCAAAGCCCAAATGCTCATAAAGCGCAATGGCTTCCTTTAAAAAAGCGGTCGTTTCCAG > NC_000913/4478882‑4479162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |