Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	170.1 / NA	58	I204S (ATC→AGC)	wcaA	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/28);  total (30/28)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926                                                                                                                                          |                                                                                                                                            tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCGGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  1:1181344/143‑1 (MQ=255) tCTGGGAGAGGGGAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  1:873102/143‑1 (MQ=255)    ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                     >  2:954495/1‑143 (MQ=255)       agagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCCTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACAAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc                                                                                                                                  >  2:454788/1‑143 (MQ=255)         agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc                                                                                                                                <  1:523288/143‑1 (MQ=255)             tAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct                                                                                                                            >  2:915202/1‑143 (MQ=255)                aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAcc                                                                                                                         >  1:457990/1‑142 (MQ=255)                  aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACttt                                                                                                                       >  1:1102801/1‑143 (MQ=255)                       atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGTTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                                  <  1:461863/143‑1 (MQ=255)                                  tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGACGTACCCGGAGAATCTTTTCGGTGCCGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTGTGCGTCGCCTCCTCTACTCTCCACGGTTCGCCGCAc                                                                                                       >  2:149039/1‑143 (MQ=255)                                  tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                       <  2:367035/143‑1 (MQ=255)                                      gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcca                                                                                                   <  1:1022340/143‑1 (MQ=255)                                          tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccac                                                                                               >  2:850274/1‑143 (MQ=255)                                             caTTGGTCTGTGTGCTGGCGGTAAAAGTGGAGGTCCCCGGAGGTTTTTTTCGGTGACGATGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCAt                                                                                            <  2:1065726/142‑1 (MQ=255)                                              aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATc                                                                                           >  2:864925/1‑143 (MQ=255)                                                     tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTGTGCTGTGCACAGCTCGCCGTCCTCCACCACCATCCGCAggg                                                                                    >  1:1382648/1‑142 (MQ=255)                                                     tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                                    >  1:107561/1‑143 (MQ=255)                                                     tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                                    >  1:566659/1‑143 (MQ=255)                                                           ttggCGGTAATAGTGGATGTACCCGGAGACTTTTTTCGGTGACGAGGTGTTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCTTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGt                                                                              <  2:1137654/140‑1 (MQ=255)                                                           cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCGACCACCATCCGCAGGAAGATGt                                                                              >  1:402058/1‑143 (MQ=255)                                                                  tataattGGCAGTACCCGTAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                       <  2:76100/137‑1 (MQ=255)                                                                  tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                       <  1:1377110/143‑1 (MQ=255)                                                                    gcgttGGACGTGCCCGGCCGATGTTTTCGGTGACGTGGTGATCTGCATTTGACCGTGATTGATATGCAGGCTCTGCTGCGCCTCTTCTTCTTTCCACGGTTCGGCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCt                                                                     <  2:903752/139‑1 (MQ=255)                                                                     aaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtg                                                                    <  1:1385284/143‑1 (MQ=255)                                                                                 ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg                                                        >  1:570026/1‑143 (MQ=255)                                                                                 ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACAATACGCAGGAAGATGGCGTACTCCTGTGCCGCTTTCAg                                                        >  1:393721/1‑143 (MQ=255)                                                                                  cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTCCAGt                                                       >  2:770358/1‑143 (MQ=255)                                                                                    gagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGTTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                     <  2:457990/143‑1 (MQ=255)                                                                                    gagaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCACCGTACTCCACCACCACCCGCAGGAAGATGTCGTAAACCTATGCCGCGTACAGTTc                                                     >  2:832537/1‑143 (MQ=255)                                                                                     agaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                    <  1:876846/143‑1 (MQ=255)                                                                                      gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATATGCAGGCTCGGCGTCGCCTCCTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCCCAGGAAGCTGTCGTACTCCCGTCCCGCCTTCAGTTCgc                                                   >  2:274831/1‑142 (MQ=255)                                                                                          tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCGCCTTTCAGTTCGGTATc                                               >  1:1244261/1‑143 (MQ=255)                                                                                           ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATGCGCAGGAAGATGTCGTACTTCTGTGCCGCTTTCAGTTCGGTATcc                                              >  1:221299/1‑142 (MQ=255)                                                                                            tttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGa                                             >  1:1269190/1‑143 (MQ=255)                                                                                               cGGTGACGTGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca                                          <  2:771470/143‑1 (MQ=255)                                                                                                ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa                                         <  1:954495/143‑1 (MQ=255)                                                                                                 gggACGGGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                        <  1:586501/141‑1 (MQ=255)                                                                                                 gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTCTCCGACCGGTACCGaacaaa                                        >  2:903207/1‑143 (MQ=255)                                                                                                 gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGCCGCCTCTACACCCAACCACGGTGCTGCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGACCCGCTTTCCGCTCGATACCGaacaaa                                        >  2:585528/1‑143 (MQ=255)                                                                                                  tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaac                                       >  1:790499/1‑143 (MQ=255)                                                                                                        ggTGCTCTGCATTTCTCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt                                 <  2:875716/143‑1 (MQ=255)                                                                                                          tGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACCTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAGTGCTGTGTACACTGCAGTTCGGTATCGAACAAACACTCTTTg                               >  2:14272/1‑143 (MQ=255)                                                                                                              cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACCAACACTCTTTGAAAc                           >  1:234979/1‑143 (MQ=255)                                                                                                               tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAAAAAACACTCTTTGAAACg                          >  2:1260706/1‑143 (MQ=255)                                                                                                                  attgtaCCGTGATTGATATGCAGGCTCTGCGTGGCGCCGCCAAGTTGCCACGGTTCGCCGTACTCCACCGCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                       <  2:1420734/138‑1 (MQ=255)                                                                                                                  aTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                       <  1:1191584/143‑1 (MQ=255)                                                                                                                     tCACCGTGATTGATATGCAGGCTCTGCGTCCCCTCTTCTACTTTCAACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTGGAAACGCCATGc                    >  1:1151791/1‑143 (MQ=255)                                                                                                                         cGTCATTGCTATGCGGGCCCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTCCCCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTGCCGCTTTCAGTTCGGCATCGACCAAACACCCTTTGAAACGCCATGCCCAg                <  2:890780/143‑1 (MQ=255)                                                                                                                              ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCAGTATCGAACAAACACTCTTTCACACGCCCTGCCCCGGTcaa           >  1:177567/1‑143 (MQ=255)                                                                                                                              ttgatAGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATTTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa           <  2:742562/143‑1 (MQ=255)                                                                                                                              tgtatatGCCGGCCCTCCGCCGCCTCCTCGCCTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGGGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa           <  2:742545/140‑1 (MQ=255)                                                                                                                              tcgctatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa           <  1:931049/139‑1 (MQ=255)                                                                                                                                 acatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc        <  2:407067/141‑1 (MQ=255)                                                                                                                                    tGCCGGCTCTGCGTCGCCCCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg     <  2:1382648/143‑1 (MQ=255)                                                                                                                                    tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTACTCCTGTGCCGCTTTCAGTTCGGTATCGACCAAACACTCTTTGAAACGCCATGCCCAGGTACAGACCTg     >  2:321598/1‑143 (MQ=255)                                                                                                                                    tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg     <  1:1390096/143‑1 (MQ=255)                                                                                                                                      cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGCACGATGTCGTAATCCTGTGCCGCTTTCCGCTCGGTATCGAACCAACACTCTTTGAACCGCCATGCCCAGGTAAACACCTGGt   >  2:1078426/1‑143 (MQ=255)                                                                                                                                       aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt  <  1:864925/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                            TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑