Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,184,543	C→A	P1100Q (CCG→CAG)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,184,543	0	C	A	100.0%	297.6 / NA	95	P1100Q (CCG→CAG)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (52/43);  total (52/43)

CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184404‑4184681                                                                                                                                            |                                                                                                                                            cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCt                                                                                                                                          >  1:989147/1‑143 (MQ=255) cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCt                                                                                                                                          <  1:1083424/143‑1 (MQ=255) cTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCt                                                                                                                                          >  1:938680/1‑143 (MQ=255)  tGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                         >  2:1180349/1‑143 (MQ=255)  tGGCGGTTAAACGCCGTATCCAGCCCGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                         <  1:747651/143‑1 (MQ=255)  gggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                         <  1:1268246/142‑1 (MQ=255)   ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGAGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGCAGCCCTCGTACTGCACCAGCAgg                                                                                                                                        >  1:360638/1‑143 (MQ=255)      ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCg                                                                                                                                     <  2:1443653/143‑1 (MQ=255)       gTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGt                                                                                                                                    >  1:952395/1‑143 (MQ=255)        ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                   >  1:881145/1‑143 (MQ=255)        ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                   >  1:712559/1‑143 (MQ=255)        ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGGCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTAAGATGAAAACGGTACGCCGGAAGACCACGTACTGAACCAGCTGGGCGTa                                                                                                                                   >  2:1093836/1‑143 (MQ=255)          aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc‑c                                                                                                                                >  1:97768/1‑143 (MQ=255)          aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGCGGGTAc‑c                                                                                                                                >  2:1371450/1‑143 (MQ=255)            aCGCCGTATCCGGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGGGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGt                                                                                                                              <  1:496645/143‑1 (MQ=255)                gggaTCCAGACTGGTTACAAGATGGCAGGTAGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCTAAGATGTGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCg                                                                                                                          <  2:1323011/140‑1 (MQ=255)                cGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGAAGACATCGTACTGAACCAGATGGGCGAAC‑Cgacccg                                                                                                                          >  2:172548/1‑138 (MQ=255)                   aTCCAGCGTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTAt                                                                                                                       <  1:563206/143‑1 (MQ=255)                    tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACTCCGGTAGACATCGTACTGAACCAGCGGGGCGTAC‑CGTCTCGTATg                                                                                                                      >  1:1376091/1‑143 (MQ=255)                    tCCAGCCTGGTGACAAGATGCCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATg                                                                                                                      <  2:1180025/143‑1 (MQ=255)                         ccTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACAt                                                                                                                 <  2:1169334/143‑1 (MQ=255)                           tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGCCCTCGTACTGCACCAGCTGGGCGTAC‑CGTCTCGTATGAACACCg                                                                                                               >  2:431356/1‑143 (MQ=255)                             gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTAAGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGGATGACCATCGGt                                                                                                             >  1:970187/1‑143 (MQ=255)                              tGACACGATGGCATGTCGTCAGGGTAGCAAAGGTGTAATTTCTAAGTTCATCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGTACCAGCTGGGCGTAC‑CGTTTCGTATTAACATCGGTc                                                                                                            <  2:40697/143‑1 (MQ=255)                                aCAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAg                                                                                                          >  1:1351792/1‑143 (MQ=255)                                      tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                    <  1:265203/143‑1 (MQ=255)                                        gCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                  <  1:123670/143‑1 (MQ=255)                                        gCAGGGCGTCACGGGAACAAGGGTGTAATGTCTACGATAAACCCGAGCGAAGATATGCCTTACGATGAAACCGGTACGCCAGTCGACAGCGCACTGCACCAGTTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGa                                                                                                  <  2:935565/143‑1 (MQ=255)                                         cAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCACCCCTCTCGAACATATGCCTTACTAGGAAGGCTGTACGCCGGTAGACATCGTACTTAACCAGCTGGGCGTAC‑CTTCTCGTATGAACATCGGTCAGATCCTCGaa                                                                                                 <  1:1308067/143‑1 (MQ=255)                                         cAGGTCGTCACGGTAACAAGGGTGTAATTTATAAGATCAACCCGATAGAGGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGaa                                                                                                 >  1:1006234/1‑143 (MQ=255)                                          aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                <  1:818946/143‑1 (MQ=255)                                          aGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATAGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                >  1:1349338/1‑143 (MQ=255)                                            gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAAcc                                                                                              >  1:1288049/1‑143 (MQ=255)                                               gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGCAC‑CGTCTCGCCTGAACCTCGGTCAGATCCTCCGAACGCAc                                                                                           >  2:1390687/1‑143 (MQ=255)                                                tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTCCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAGACCCAcc                                                                                          >  2:954543/1‑143 (MQ=255)                                                 cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                         >  1:893078/1‑143 (MQ=255)                                                      tAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTa                                                                                    <  1:1018162/143‑1 (MQ=255)                                                       aaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACGTGGGTAt                                                                                   >  1:214448/1‑143 (MQ=255)                                                          aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGCACGGCGGTCAGCTCCTCGAAACCCACCTGGGGATGGc                                                                                >  1:266754/1‑143 (MQ=255)                                                          aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                                >  2:589771/1‑143 (MQ=255)                                                          aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGc                                                                                >  1:1131053/1‑143 (MQ=255)                                                          aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTCGGTATGGc                                                                                >  2:611556/1‑143 (MQ=255)                                                           aGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCt                                                                               <  1:663174/143‑1 (MQ=255)                                                            gggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGACTGGGTACGGCTg                                                                              >  2:763210/1‑143 (MQ=255)                                                             ggTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGc                                                                             <  2:1147035/143‑1 (MQ=255)                                                               tgtAATTTCTAAGTTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                           <  1:185357/143‑1 (MQ=255)                                                               tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTCGGCGTAC‑CGTCTCGTATGAAAATCGGTCAGATCCTCGAAACCCACCTGGGCATGCCGGCgc                                                                           >  2:270949/1‑142 (MQ=255)                                                                gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaa                                                                          >  2:524450/1‑143 (MQ=255)                                                                 tAATTTCTAAGATCAACGCGATCGAAGATATGCCTTACGATGAGGGGGGTGCGCCACCACACATCGTTCTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  2:308748/143‑1 (MQ=255)                                                                 tAATTTCTAAGATCAACCCGATCGAAGATGTGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                         <  2:985255/143‑1 (MQ=255)                                                                   aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGTTGTAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGCACATCGGTCAGATCTTCGAAACCCACCTGCGTATGCCTGCGAAAgt                                                                       >  2:519626/1‑142 (MQ=255)                                                                    tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGt                                                                      >  2:592233/1‑143 (MQ=255)                                                                       cTAAGATCAGCCGGATGGAAGTTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTTGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATc                                                                   <  2:1169509/143‑1 (MQ=255)                                                                         aaGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCgg                                                                 >  1:1243698/1‑143 (MQ=255)                                                                         aaGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCgg                                                                 >  1:575372/1‑143 (MQ=255)                                                                          acaTCAACCCCATCGAAGACCTGCCCTACGATCAACACGGTTGGCTGGTAGACACCGGACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGc                                                                <  1:49554/141‑1 (MQ=255)                                                                            aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                              <  2:164692/143‑1 (MQ=255)                                                                             tCAAGCCGATCGAAGAGATGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                             <  1:1284404/143‑1 (MQ=255)                                                                             acAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCAGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                             <  1:1056045/142‑1 (MQ=255)                                                                                  ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                        >  1:217759/1‑143 (MQ=255)                                                                                    gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCCGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCa                                                      >  2:1002321/1‑143 (MQ=255)                                                                                    gATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTAAACCAGCTAGGGGTAC‑AGTCTCGTATGAACATCGGCGAGAACCTCGCAACCCAGCACAGTATGCCTACGGAAGGCATGGCGGACAACAtaa                                                      >  2:1023409/1‑141 (MQ=255)                                                                                    gATAGAATATATGCATTACGATGAAAGCGGGACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCa                                                      <  2:562455/143‑1 (MQ=255)                                                                                      tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGACCCATCGGGGCGCACACGT‑ACTAATTATCTTCTGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    <  2:795588/143‑1 (MQ=255)                                                                                      tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    <  1:178191/143‑1 (MQ=255)                                                                                      tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                    >  1:626934/1‑143 (MQ=255)                                                                                        gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGGCAAGATCAACGc                                                  >  1:857150/1‑143 (MQ=255)                                                                                         aaGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGACCATCGGTCAGATCCTCGAAACCCCCCTGGGTCTGGCTGCGAAAGCTATCGGCGACAAGACCAACGcc                                                 >  2:745702/1‑143 (MQ=255)                                                                                          aGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGACACCCGCCTGGGTATGGAAGCGAAATCCTGCGGCGACAAGATAAACGCCa                                                >  1:914480/1‑143 (MQ=255)                                                                                                gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGa                                          <  2:472047/143‑1 (MQ=255)                                                                                                gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCGGGGTATGGCAGCGAAAGGTAGCGGCGACGCGAACAACGGCATGcgaa                                          >  2:1320387/1‑140 (MQ=255)                                                                                                 ccTGACGATGAAAACGGTACGCCTGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaa                                         <  1:227439/143‑1 (MQ=255)                                                                                                  cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATTGGCGACAAGATCAACGCCATGCTGaaa                                        <  1:1414460/143‑1 (MQ=255)                                                                                                  cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAGCGGCGACAAGATCAACGCCATGCGGaaa                                        >  2:1138039/1‑143 (MQ=255)                                                                                                   ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                       >  1:201933/1‑143 (MQ=255)                                                                                                      cGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                    <  1:7347/143‑1 (MQ=255)                                                                                                       gATGAAACCGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                   <  1:1236736/143‑1 (MQ=255)                                                                                                         tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCGCGTATGAACATCGGTCAGATGCTCGAAACCCACCTGGGTATGCCTGCGAAAGGTCTCGGCTACAAGATCAACGCCATGCCGAAACaacagc                                 >  2:306947/1‑143 (MQ=255)                                                                                                         tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCCTAC‑CGTCTCGTATGACCATCGGTCAGATCCTCGAAACCCACCTGGGTATCAATGAGCGACGTATCGGCGACAAGATCAACGCCATGCTAACAccgcacc                                 >  2:304756/1‑141 (MQ=255)                                                                                                              gcGGTACGCCGGTCGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                            <  1:1222130/142‑1 (MQ=255)                                                                                                              aCGGTTCGCCCGTAGACCTCTTAGTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                            <  1:220790/143‑1 (MQ=255)                                                                                                                  tACGCCGGTACACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGGCAGATCCTCGAAGCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                        <  2:830801/143‑1 (MQ=255)                                                                                                                        ggTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                  >  1:696576/1‑143 (MQ=255)                                                                                                                        ggTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                  <  2:1243698/143‑1 (MQ=255)                                                                                                                         gTAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACt                 >  1:1317958/1‑143 (MQ=255)                                                                                                                          tAGACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTg                <  1:589771/143‑1 (MQ=255)                                                                                                                           agactgcGTACTGAACCAGTTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAGCTGGGTATGGATGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc               <  2:712559/137‑1 (MQ=255)                                                                                                                            gACATCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg              <  1:1155183/143‑1 (MQ=255)                                                                                                                               aTCGTACTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCCGCGACAAGATCAACGCCATGCTGGAACAGCCGCAAGAAGTCGCGAAACTgcacaa           >  2:457608/1‑139 (MQ=255)                                                                                                                                tCGTACTGAACCAGCTGGGCGTAC‑CGTCCCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGACAGGTCTCGGCGACCAGACCCACGCCCTGCCGAAACAGCAGCAAGCAGTCGCGAAACTGCGCaaa          >  1:20204/1‑143 (MQ=255)                                                                                                                                    aCTGACCCAGCCGGGCGGCC‑CGTCTCGTATGAACCTCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa      <  2:715574/143‑1 (MQ=255)                                                                                                                                     cTGAACCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGCATGGCGGCGAAAGGTATCGGCGACAAAATCAACGCCATGCTGGAACAGCAGAAAGAAGTCGCGAAAATGCGAGAAATcgt     >  2:835049/1‑141 (MQ=255)                                                                                                                                        aaCCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGCAAATGCGCTACTTCATCCa  >  2:427588/1‑143 (MQ=255)                                                                                                                                        aaCCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCa  <  2:1078128/143‑1 (MQ=255)                                                                                                                                        aaCCAGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATGCATCCa  >  1:1039136/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                            CTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTAC‑CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA  >  NC_000913/4184404‑4184681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑