Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	459,834	C→A	A316E (GCG→GAG)	lon →	Lon protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	459,834	0	C	A	98.5%	185.0 / NA	70	A316E (GCG→GAG)	lon	Lon protease
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (33/34);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGCGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTA  >  NC_000913/459701‑459971                                                                                                                                       |                                                                                                                                          gAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAAt                                                                                                                                  >  1:890125/1‑143 (MQ=255)     cAGCGTTGCAGAAGCTGAAACTGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCtt                                                                                                                              <  1:121934/143‑1 (MQ=255)      agagTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTg                                                                                                                             <  2:55971/143‑1 (MQ=255)       gagTTTCAGAAGCTGAAAAGGATGTCTCCGATGTCGGAAGAAGCGACCGTAGGGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGa                                                                                                                            <  1:1284684/143‑1 (MQ=255)        agTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTGGTGCGTGGTTATATCGACTGGATGGGACAGGTGCCGTGGAATGCGCGTAGCAAGGGCAAAAAAGACCTGCGT‑CAGGAGCCGGCAAGCCTTGAt                                                                                                                           >  1:96019/1‑143 (MQ=255)          ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATAc                                                                                                                         >  2:545462/1‑143 (MQ=255)             cAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGa                                                                                                                      <  1:631935/143‑1 (MQ=255)              aGAAGCCGAAAATGATGTCCCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGAc                                                                                                                     <  1:556922/143‑1 (MQ=255)                    tGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTAt                                                                                                               >  1:652139/1‑143 (MQ=255)                     gAAGATGCTGTCTGCGATGTCGGCTGAAGGGACGGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATg                                                                                                              <  2:821165/143‑1 (MQ=255)                     gAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTATAGGTGAAGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGAACATCCTTGATACCGACCATTATg                                                                                                              >  2:174678/1‑143 (MQ=255)                             tgTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGGCTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGAGCATTATGGTCTGGAg                                                                                                      >  1:1029908/1‑143 (MQ=255)                              gTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAACGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATCGTCTGGAgc                                                                                                     >  2:246416/1‑143 (MQ=255)                               tctcCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGGGGAATGCGCGTAGCAAGGTCGAAAACGACCTGCGC‑CAGGAGCAGGAAATCCTTGATACCGGCCATTAGGGTCggggggg                                                                                                    >  1:880016/1‑139 (MQ=255)                               tctcCGACGTCCGCAGAAGCGACCGTAGTGCGCGGTTATATCGACTTGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAgcg                                                                                                    <  1:1468444/143‑1 (MQ=255)                                 tctgATGTCGGCAGAAGCGACCGGAGTGCGTGGTTGTATCGACTGGATGGTACAGGTGCCGGGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGcgcg                                                                                                  <  2:1253854/140‑1 (MQ=255)                                 tcCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGGGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGGGCAGGAAATCCTTGATACCGCCCACTATGGTCCGGAGcgcg                                                                                                  >  2:716294/1‑143 (MQ=255)                                  ccGGTGTCGGCAGAAGCGACCGTAGTGCGTGGTGAGAGCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGt                                                                                                 <  2:343933/143‑1 (MQ=255)                                   cGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCTGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTg                                                                                                >  2:776129/1‑143 (MQ=255)                                     aTGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGaa                                                                                              >  1:834161/1‑143 (MQ=255)                                      tGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGaaa                                                                                             <  2:216751/143‑1 (MQ=255)                                        tCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATACTTGATACCGACCATCATGGTCTGGAGCGCGTGAAAGa                                                                                           >  2:853810/1‑143 (MQ=255)                                         cGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGAt                                                                                          <  2:890125/143‑1 (MQ=255)                                          tgaaGACGCGACTGTAATGAGTGGTTATATTGACTGGATGCTACAGGTGCCGTGGAATGCGCGTAGCAAGAGCAAAGAATACTTGACT‑TGGCAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATc                                                                                         <  2:518552/140‑1 (MQ=255)                                          tccAGAAGCGCCCGTAGTGCGTGGTTATATCGACTGGCTGGCGCAGGTGCCGTGGAATTCCTTCATCTTAGTCAACAACGACCCGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATc                                                                                         <  1:1458548/141‑1 (MQ=255)                                          ggaggAAGCGATCGTGGTGCGTGGTTATATCGAGAGAATGGTAGAGGTGGCGTGGAATGCGCGTAGCAAGGTCAAAAAAGATCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATc                                                                                         <  2:884242/139‑1 (MQ=255)                                          ggCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGTGCAGG‑GCGTAAAATCCTTGATACCGACCGTTATGGTCTGGCGCGCGTGAAAGATc                                                                                         >  2:1039022/1‑143 (MQ=255)                                          ggCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATc                                                                                         <  2:457670/143‑1 (MQ=255)                                           gCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCg                                                                                        >  2:1200143/1‑143 (MQ=255)                                                 gCGACCGTAGTGCGTGGGTATATCGACGGGATGGTACAGGTGCCGTGGAATGCGCGGGGCAAGGGCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGCGAAAGATCGAATCCt                                                                                  >  1:1197962/1‑143 (MQ=255)                                                    tgcggaGTGCGTGGTTGTATCGACTGGGTGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGGCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGGGTGGAGCGCGTGAAAGATCGAATCCTTGa                                                                               <  2:681652/138‑1 (MQ=255)                                                    gaggtggTGCGTGGTTATATCGACTGGAGGGTACAGGTGCCTGGGAATGCGCGTAGCAAGGTCATAAAAGCCCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGa                                                                               <  2:173630/137‑1 (MQ=255)                                                    aCCGTAGTGGGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGa                                                                               <  2:32297/143‑1 (MQ=255)                                                        tAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCGTGCGTAt                                                                           >  1:197245/1‑143 (MQ=255)                                                           tGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGGCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGGTCGAACGCTCGAGTATCtt                                                                        >  2:198061/1‑143 (MQ=255)                                                            gggtagTTATCTCGACTGAATAGTACAGGTGCCATGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTg                                                                       <  2:660243/138‑1 (MQ=255)                                                             cGTGGTTATATCGACTGGAGGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CGGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGc                                                                      <  2:608045/143‑1 (MQ=255)                                                                ggTCATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGt                                                                   >  2:815509/1‑143 (MQ=255)                                                                  tgatgtCGACTTGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTc                                                                 <  1:1640/138‑1 (MQ=255)                                                                   tataTCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAGGATCGAATCCTTGAGTATCTTGCGGGTCa                                                                >  2:403857/1‑143 (MQ=255)                                                                     tatCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAATGACCTGCGT‑CAGGAGCAGGAATTTCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGACTCCTTGAGTATCTTGCGGTTCaaa                                                              <  1:362217/143‑1 (MQ=255)                                                                      atCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGGCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTGTGGGCTGGAGCGCGTGATAGATCGGAGCCTTGGGTATTTTGCGGTTCGAAg                                                             >  1:496363/1‑143 (MQ=255)                                                                       tCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCCAGGTCAAAAAAGACCTGCGT‑CAGGCGCAGGCAATCCTTGATACCGACCATTATGGACTGGAGCGCGTGAAAGATCAAATCCTTGAGTCACTTGCGGGTCAAAGc                                                            >  2:1152721/1‑143 (MQ=255)                                                                       tCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGc                                                            >  1:1469902/1‑143 (MQ=255)                                                                          aCTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCgt                                                         >  1:235731/1‑143 (MQ=255)                                                                            gggAGGGTACAGGTGCCGTGGAATGCGCGTAGCGAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCgtgt                                                       <  2:516090/142‑1 (MQ=255)                                                                               aTGGTACAGGTGCGGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaa                                                    <  2:1443204/143‑1 (MQ=255)                                                                               aTGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACTTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaa                                                    >  2:102015/1‑143 (MQ=255)                                                                               aTGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGACTCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaa                                                    >  2:783636/1‑143 (MQ=255)                                                                               aTGGTAACGGTGGCGTGGAATGCGAGCAGCAAGGTCGAAAAAGCCCTGCGT‑CAGGACGAGGAAATCCTAGAGACCGACGATGACGGTCTGGGGCGCGTGAAAGCTCGAATCCGTGAGTATCTTGCGGTTCAAAGCCGTGTcaa                                                    <  2:511419/143‑1 (MQ=255)                                                                                    aCAGGTGCCGTGGAATGCGCGTAGCAAGGACAAAACAGGCCTGCGT‑GAGGAGCAGGAAATCCTTGCTATGAGGTATTATGGTCTGGAGCGCGGGAAGGATCGAGTGCTTGAGTATCTTGCGGTTCAAGGCGGTGTCAACaaaa                                               >  2:1042569/1‑143 (MQ=255)                                                                                     cAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAAt                                              <  1:1125960/143‑1 (MQ=255)                                                                                      aGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGGTCAAAGCCGTGTCAACAAAATc                                             >  1:1026331/1‑143 (MQ=255)                                                                                        gTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTCTGTTGCGGTTCAAAGCCGTGTCAACAAAATcga                                           >  1:392660/1‑141 (MQ=255)                                                                                        gTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCaa                                           <  1:1040932/143‑1 (MQ=255)                                                                                        gTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGACCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCaa                                           <  1:9800/143‑1 (MQ=255)                                                                                         tGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGCCAACAAAATCaac                                          >  2:327853/1‑142 (MQ=255)                                                                                             gTGGAATGCGCGTAGCAAGGTCAAAAAGGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTc                                                                 <  2:1130185/116‑1 (MQ=255)                                                                                             gTGGAATGCGCGTAGCAAGGTCAAAAAGGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTc                                                                 >  1:1130185/1‑116 (MQ=255)                                                                                                            caggctCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTCGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTgg                       <  2:1267882/138‑1 (MQ=255)                                                                                                            cAAGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTgg                       >  1:1048248/1‑143 (MQ=255)                                                                                                            cAAGGCCAAAAACGACCTGCGT‑CAGGAGCAGAAAATCCTTGATACCGACCATTATGGGCTGGGGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTGCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTgg                       <  2:1109901/143‑1 (MQ=255)                                                                                                              agctCAAACAAGCCCCGCGG‑CAGGAGCAGGAAATCCTTGCTACCGACCATTATGGTCTGGACCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTa                     <  1:1364808/140‑1 (MQ=255)                                                                                                              aGGTCAAAAAAGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGTACCGATCCTCTGTCTGGtc                     >  1:517286/1‑142 (MQ=255)                                                                                                                 ccAAAAAAGCTCTGCGT‑CAGGATCAGGAAATCGTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAggg                  <  2:1125938/142‑1 (MQ=255)                                                                                                                       aaGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATAATGGGGTGGAGCGCGTGAGAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCGAGGGACCGATCGTCTGCCTGGTAGGGccgccg            >  1:775764/1‑143 (MQ=255)                                                                                                                        aGACCTGCGT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTATGGAGCGCGTGAAAGATCGAATCCTAGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCgg           >  1:1029091/1‑143 (MQ=255)                                                                                                                                gT‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGggtaggt   <  1:90340/143‑1 (MQ=255)                                                                                                                                 t‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGTCGCGCTTGAAAGATCGAATCCTCGAGTCTCTTGCGGTTCAAAGCCGTGTCAACAAAATCCAGGGCCCGATCCTCTGCCTGGAAGGGCCGCCGGGggtaggtg  >  1:1007039/1‑142 (MQ=255)                                                                                                                                 t‑CAGGAGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGggtaggta  >  1:925139/1‑143 (MQ=255)                                                                                                                                       |                                                                                                                                          GAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGGATGGTACAGGTGCCGTGGAATGCGCGTAGCAAGGTCAAAAAAGACCTGCGT‑CAGGCGCAGGAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGTAGGGCCGCCGGGGGTAGGTA  >  NC_000913/459701‑459971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑