Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	97.2%	196.8 / NA	74	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base T (35/35);  minor base G (1/1);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696331‑696607                                                                                                                                            |                                                                                                                                          gTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGa                                                                                                                                        <  2:1052289/143‑1 (MQ=255)  tCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTGTTAATGTGGCTTGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGaa                                                                                                                                       <  1:523565/143‑1 (MQ=255)      aTCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCg                                                                                                                                   >  1:437712/1‑143 (MQ=255)          gACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                               >  1:811132/1‑143 (MQ=255)          gACAGTGGAGGAGAAGACGTGTCAACATCGCATTCGACATTGAAGAAAGGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGc                                                                                                                               <  1:323194/143‑1 (MQ=37)            cATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCt                                                                                                                             <  1:1423461/143‑1 (MQ=255)             aTTGAAGGATAAGCCGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCtt                                                                                                                            <  2:1329895/143‑1 (MQ=255)              gtGAGGGATAGGACGTGTCGACATCGCATTCGACGTTGAATGAGCGCAGAAAAGCAAAGAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTa                                                                                                                           <  2:1335749/142‑1 (MQ=37)               tGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTAc                                                                                                                          <  2:1143656/143‑1 (MQ=255)                 aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg                                                                                                                        >  1:130301/1‑143 (MQ=255)                 aaGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGAGGTACGAGGATTCGAACCTCGGCATGTCGGAATTAGACTCCGGTGCCTTACCg                                                                                                                        >  2:1414055/1‑143 (MQ=38)                    gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt                                                                                                                     <  2:234413/143‑1 (MQ=255)                    gATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATACGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCtt                                                                                                                     >  1:1139482/1‑143 (MQ=255)                     atcaGACGTGTCAACATCGAATTTGACATTGAATGAACGCAGAAAAGCAAAAGGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTg                                                                                                                    <  1:1376193/140‑1 (MQ=37)                     aTAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTg                                                                                                                    <  1:1376314/143‑1 (MQ=255)                      tAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                   >  1:785775/1‑143 (MQ=255)                       aaGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGCATGCCGGAATTAGAATCCGGTGCCTTACCCCTTGGc                                                                                                                  >  2:1055039/1‑143 (MQ=255)                         gACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGCAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACATCGGAACGCCGGAATTAGAATCCGGTGCCTTACAGCTTGGCGa                                                                                                                >  2:1080369/1‑143 (MQ=37)                             tgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGGATCAGAATCCGGTGCCTTACCGCTTGGCGATAcg                                                                                                            >  2:681602/1‑142 (MQ=255)                             tgtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAGTCCGGTGCCTTACCGCTGGGCGATAcc                                                                                                            >  2:857051/1‑143 (MQ=255)                              gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGACGGGATGATAATCCGGTGCCTTACCGCTTGGCGATAccc                                                                                                           >  2:642100/1‑143 (MQ=255)                                 aaCATCGCATTCGACAGTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaa                                                                                                        >  1:1025836/1‑143 (MQ=255)                                  aCATAGCATTCGACATTGAATGAATGCAGAAAAGCAAAAAGCGCGCCGAAGCGAGCGGTTTGAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAc                                                                                                       <  1:351170/143‑1 (MQ=21)                                  aCAGCGCATTCGTCATTGAATGATCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGTCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAc                                                                                                       <  1:1290381/143‑1 (MQ=25)                                     tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGCATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCCACTgg                                                                                                    >  1:665593/1‑143 (MQ=255)                                     tCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                                                                                    <  1:955241/143‑1 (MQ=255)                                     gcgcggtCGGCAGTGAATGGGCGCAGAAGAGCGAGAAGGTCGGCGAGGCGAGCTTGTTTAATGTGGGTGGGGTACGGGGATTCGAACCTGGGAGTGCCGGAGTTAGAATCCGGTGCCTGACCGCTTGGCGATACCCCAACTgg                                                                                                    <  2:338413/137‑1 (MQ=12)                                      cGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACCggg                                                                                                   >  2:401449/1‑143 (MQ=255)                                       gCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGt                                                                                                  <  2:993049/143‑1 (MQ=255)                                          ttCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCa                                                                                               >  2:134051/1‑143 (MQ=255)                                             gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACtt                                                                                            <  2:561620/143‑1 (MQ=255)                                             gACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACtt                                                                                            <  1:705609/143‑1 (MQ=255)                                              aCATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                                                                                           >  1:1413666/1‑143 (MQ=255)                                                aTTGAACGAACGCAGAAAAGCAAAAAGGCCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAAGTAGAATCCGGTGCCTTAGCGCTTGGCGATACCCCAACTGGGTGCACTTACa                                                                                         <  1:153936/143‑1 (MQ=25)                                                 ttGAATGAACGCAGAAAAGCAAGAAGCGCGGCGAAGCGAGCTTCTTTAACGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                                        <  1:603776/143‑1 (MQ=21)                                                 ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                                                                                        <  2:1252454/143‑1 (MQ=255)                                                 ttGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACCGAGAGCACTTTCat                                                                                        >  2:521209/1‑142 (MQ=21)                                                  tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTAcccg                                                                                       >  1:304662/1‑140 (MQ=255)                                                  tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                                                                                       >  1:788062/1‑143 (MQ=255)                                                   gaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGGGCACTTACAAgg                                                                                      >  2:1127413/1‑143 (MQ=255)                                                     atgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGAGGAGTGTCAAGGTa                                                                                    >  2:1262657/1‑143 (MQ=21)                                                       gaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGCTACCCCAACTGGGTGCACTTACAAGGTAAg                                                                                  >  1:1345719/1‑143 (MQ=255)                                                            cAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCt                                                                             <  1:1419381/143‑1 (MQ=255)                                                              gAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCCACTGGGTGCACTTACAAGGTAAGCGTCTTg                                                                           >  1:861622/1‑143 (MQ=255)                                                               aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGGGTCtcgc                                                                          >  1:565972/1‑140 (MQ=255)                                                               aaaaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAGTGGACTTCCAAGGTACGCGTATTGa                                                                          >  2:624106/1‑143 (MQ=18)                                                                 aaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAAt                                                                        >  1:404295/1‑143 (MQ=255)                                                                 aaGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAAt                                                                        >  1:751348/1‑143 (MQ=255)                                                                    cAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACACGGTAAGCGTATTGAATata                                                                     >  2:74954/1‑141 (MQ=255)                                                                         aGCTCGCCGAAGCGAGCTTCTTTCATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGc                                                                <  1:1286323/143‑1 (MQ=255)                                                                             cgcgGAAGCGAGCTTTTTTAATGTGGCTGGGGTAGGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTggg                                                            <  2:43124/143‑1 (MQ=255)                                                                                      gAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACATGGTAAGCGTCTTGACTAAATTTGCTGGGTTACGAggc                                                   >  1:501022/1‑142 (MQ=21)                                                                                       atcttcttTAGTCTGGCTGGGGGACGAGGTTTCGAGCCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATATCCTAACTGGGTGCACGTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGAt                                                  <  2:967742/141‑1 (MQ=21)                                                                                          ttttttACTGTGGCTGTGGTAGGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCg                                               <  1:1093044/143‑1 (MQ=255)                                                                                           ttgttAGTGTGGGTGGGGTCGGGGGATTCGAGCTTTGGATTGCAGGAATTAGTGCGCGGTGCATTATGGCTTGGAGGTACCCCAACTGGGTGCACTTAACCGATAAACCGGGTCTGGAAATTGGCTGGGGTACGAGGATTCGa                                              <  2:1114423/140‑1 (MQ=255)                                                                                               tAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCACCACCAGCGCCCCGTACAAGGTACGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCt                                          >  1:1457370/1‑143 (MQ=18)                                                                                                aaTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                   >  1:455712/1‑69 (MQ=255)                                                                                                aaTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                                                                                                   <  2:455712/69‑1 (MQ=37)                                                                                                      gCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATg                                   <  1:1050463/143‑1 (MQ=255)                                                                                                       cTGGGGTACGGGCATTCGTGCCTTGGAATGCTGGGATGAGTATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAATGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGc                                  <  2:386761/143‑1 (MQ=37)                                                                                                        tGGGGTACGAGGGTTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                 <  1:1251589/143‑1 (MQ=255)                                                                                                         ggggTACGAGGATTCGAACCGCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCg                                <  1:360383/143‑1 (MQ=255)                                                                                                               cGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCa                          <  1:1272905/143‑1 (MQ=255)                                                                                                                 aGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTACATTGGACGCGCATTGTCTAAAGTGGCGGGGGTCCGAGGGTAGGAACCGCGGATTGGCGGaagcgga                        >  2:1212194/1‑138 (MQ=9)                                                                                                                 aGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTCAAAGGTCAGCGTCGTGAAAGCATCGGCTGGAGCACGAGCACTCGAACCACGGAATGCCGGAATCAGa                        >  1:27753/1‑143 (MQ=18)                                                                                                                        gAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAATGTAAGCGTATTGACTAAATTGGCTGGGGTACGAGGATTCGAACCTCATAGTGCCGGAAACAGAATCCgtt                 >  2:1002423/1‑141 (MQ=25)                                                                                                                        gAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGTGCACTTACAAGGTAAGCGCCTTGACTAAATTGGCTGGGTTACGAGGATTCGAACCGCGGCATGCCGGAATCACACTCCggg                 >  2:264277/1‑142 (MQ=25)                                                                                                                          aCCTCGGAATGCCGGCATTAGAATCCGGTGCCTTCCCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc               <  1:443495/143‑1 (MQ=255)                                                                                                                               ggcacgCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAc          <  1:708251/138‑1 (MQ=255)                                                                                                                                  aTGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTAAACAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc       <  1:401449/143‑1 (MQ=255)                                                                                                                                     cgggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg    <  1:1347443/141‑1 (MQ=255)                                                                                                                                      cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGGACTTACAGGATAAGCGTCTTGAATAAAGGGGCTGTGGGACGAGGATTCGAACCACGGAAGGCCGGGATCAGAATCCGGGGCCTTACCgagtgg   >  1:655656/1‑138 (MQ=25)                                                                                                                                       ggAATTAGAATCCGGTGCGTTACGGCTTGGCGATACGCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc  <  1:902502/143‑1 (MQ=255)                                                                                                                                       ggAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACCAGGTGCACTCACAAGGTAAGCGCCTTGAATAAATTGGCTGGGGTGCGAGGATTCGAACCTCGGAATGCCGGGATGCGAATCAGTTGCCTTAGCGCTTGGc  >  2:627107/1‑143 (MQ=25)                                                                                                                                            |                                                                                                                                          GTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGC  >  NC_000913/696331‑696607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑