Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,446	A→G	L8P (CTC→CCC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,446	0	A	G	98.5%	185.5 / NA	66	L8P (CTC→CCC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (31/33);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGG  >  NC_000913/754308‑754580                                                                                                                                           |                                                                                                                                       tCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGttt                                                                                                                                    >  2:185029/1‑143 (MQ=255) tCGCAGGATGCGGTTCAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGttt                                                                                                                                    >  2:1239581/1‑143 (MQ=255)        acgCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                             <  1:398030/141‑1 (MQ=255)        aTGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCtttt                                                                                                                             <  2:306584/143‑1 (MQ=255)          gCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGt                                                                                                                           <  1:632410/143‑1 (MQ=255)             gTTGCAGTGACGCCTGGGTCAAAGGTGAACACCCCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACTCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATc                                                                                                                        <  1:588561/143‑1 (MQ=255)                  agtgaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTTACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCa                                                                                                                   <  1:652752/143‑1 (MQ=255)                     gaagCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCCTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCGGTATCCCCGTTCAGGGTGGGTGTGGCTTTTGCACCAGCAAttt                                                                                                                >  2:1386667/1‑143 (MQ=255)                      aagCCTGGGTCAAACGTTAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCGGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTa                                                                                                               <  2:935524/143‑1 (MQ=255)                       agCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                              <  1:1064996/143‑1 (MQ=255)                       agCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTaa                                                                                                              <  1:1459384/143‑1 (MQ=255)                        gCCTGGGTCAAAGGTGAACCCTCCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                             <  2:1168236/143‑1 (MQ=255)                        gCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAg                                                                                                             <  1:471507/143‑1 (MQ=255)                            ggtgcAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCATCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGtct                                                                                                         <  2:615132/139‑1 (MQ=255)                                cAAAGGGGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCAAGTTCGTCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCtt                                                                                                     <  1:208404/143‑1 (MQ=255)                                 aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCGCATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCttg                                                                                                    >  2:691796/1‑142 (MQ=255)                                 aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTa                                                                                                    >  2:184427/1‑143 (MQ=255)                                 aaaGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTACCAGCCATTTAAGGTCTCCTTa                                                                                                    >  2:402907/1‑143 (MQ=255)                                     gTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTGTCCTTGgcgc                                                                                                >  1:158294/1‑143 (MQ=255)                                     gTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTGGAGGGTGGGTTTTGCTGTTGTATAAGCCATTTAAGGTCTCCTTAgcgc                                                                                                >  1:161834/1‑143 (MQ=255)                                           acacCTTTTGAACCGAGAGTACGGATATCGATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTAtt                                                                                          <  1:1050492/143‑1 (MQ=255)                                            cacCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTg                                                                                         <  1:1332291/143‑1 (MQ=255)                                                ttttGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTCAGGTCTCCTTAGCACCTTATTCCGTa                                                                                     >  1:409794/1‑143 (MQ=255)                                                ttttGAACCGAGAGTACGGATATCAATAACATCTCGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCCTTTAAGGTCTCCTTAGCGCCTTATTGCGTa                                                                                     >  2:323458/1‑143 (MQ=255)                                                   tGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGa                                                                                  <  2:1130874/143‑1 (MQ=255)                                                      aCCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTg                                                                               <  1:184427/143‑1 (MQ=255)                                                               aCGGATATCAATAGCATCTTGGCCCGGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACtt                                                                      <  2:1111727/143‑1 (MQ=255)                                                               aCGAATATCAATAACATCTTGACCCATCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACtt                                                                      <  2:1376044/143‑1 (MQ=255)                                                                 ggATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTaa                                                                    <  2:1138947/143‑1 (MQ=255)                                                                     atCAATACCATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAAttt                                                                <  2:1339801/143‑1 (MQ=255)                                                                      tCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATTAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTACGACTGCCGGAACTTAAATTTg                                                               >  2:1321616/1‑143 (MQ=255)                                                                       cAATAACATCTTGTCCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGc                                                              <  2:116708/143‑1 (MQ=255)                                                                          tAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTGAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCtt                                                           >  1:1176713/1‑143 (MQ=255)                                                                           attaTCTTGACTCAGCGTGCCTTTCAGCAAATCCAGTTCAACAGCTGTAGGCCCGGTGAGGGTGGGTTTGGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTc                                                          <  1:320391/140‑1 (MQ=255)                                                                           aaCATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTACATTTGCCTTc                                                          >  2:893715/1‑143 (MQ=255)                                                                           aaCATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTc                                                          >  1:590161/1‑143 (MQ=255)                                                                            aCAGCTTGACCCCGCGTGCCTTTCAGCACATCCAGTTCAACAGCGGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCg                                                         <  1:82171/143‑1 (MQ=255)                                                                              aTCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGca                                                       >  1:1238066/1‑143 (MQ=255)                                                                                     cccgccGTGCCGCTCAGCACATCCAGTTCAACAGCTGGACCCCCGTTGAGGGTGGGTTTTGCTTTTGTAGCATCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAAc                                                <  1:46987/138‑1 (MQ=255)                                                                                       cAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCCCACATCAACCt                                              >  2:313062/1‑143 (MQ=255)                                                                                           gTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCt                                          >  1:1354583/1‑143 (MQ=255)                                                                                            tGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCtt                                         <  1:1239581/143‑1 (MQ=255)                                                                                            tGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGAtt                                         >  1:1345287/1‑143 (MQ=255)                                                                                               cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTACGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAc                                      >  2:405867/1‑143 (MQ=255)                                                                                               cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTgcccttcc                                      >  1:481089/1‑136 (MQ=255)                                                                                               cTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAAATTAAATTTGCCTTCGCACATCAACCTGGCCTTAc                                      >  2:433460/1‑143 (MQ=255)                                                                                                tttCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTAcc                                     >  2:1219160/1‑143 (MQ=255)                                                                                                    aGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGtt                                 >  2:883140/1‑143 (MQ=255)                                                                                                     gCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttt                                >  2:898163/1‑143 (MQ=255)                                                                                                       acaTCCAGTTCACCAGATGTATGCACGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGttttt                              <  1:1376352/143‑1 (MQ=255)                                                                                                            cAGTTCAACGGAGGTATCCCCGTTGAGGGTGTGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                         <  2:1261944/143‑1 (MQ=255)                                                                                                            cAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTCTTGCGTAAAACTGCCGGAACATAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTATAttt                         >  1:158956/1‑143 (MQ=255)                                                                                                            cAGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTAttt                         <  1:185029/143‑1 (MQ=255)                                                                                                             aGTTCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTg                        >  2:1431147/1‑143 (MQ=255)                                                                                                              gtgcaccAGCTGTATCCCGGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTCCCCGTTTTTTATTTgg                       <  2:903270/137‑1 (MQ=255)                                                                                                              gTTCAGCAGCTGTATCCCCGTTGAGGGTGGGTTTTGCGTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTgg                       <  2:485690/143‑1 (MQ=255)                                                                                                                tCAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCCGCCCTTTAAGGTCTCCTTAGCGCCTTATTGCGTCAGACCGCCGGGACCTAAATTTGCCTTCGCACATCAACCCGGCTCTACCCGTTTTTTATTTTGcc                     >  2:1455155/1‑142 (MQ=255)                                                                                                                 cAACAGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTc                    >  2:781970/1‑143 (MQ=255)                                                                                                                    cAGCTGTATCCCCGTTGAGGGTGGGTTTTGCATTGTGATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTcgcc                 <  1:609537/143‑1 (MQ=255)                                                                                                                     aGCTGTATCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTGAATTTGCCTTCGCACATCAACCTGGCTTTCCACGTTTTTTTTTTTGCTcgccg                >  2:1142385/1‑143 (MQ=255)                                                                                                                           aTCCCCGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCtgt          <  1:76181/143‑1 (MQ=255)                                                                                                                             ccccGTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTCCCCGTTCTTTATTTGGCTCGACGCTCtgtgg        >  1:888864/1‑142 (MQ=255)                                                                                                                               ccGTGGAGTGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAACTTTGCCTGCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGaaa      <  2:532853/143‑1 (MQ=255)                                                                                                                                 gTTGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAaga    >  1:463764/1‑143 (MQ=255)                                                                                                                                  ttGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAagag   <  1:1418873/143‑1 (MQ=255)                                                                                                                                   tGAGGGTGGGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAgg  <  1:662265/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       TCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTGAACACACCTTTTGAACCGAGAGTACGGATATCAATAACATCTTGACCCAGCGTGCCTTTCAGCACATCCAGTTCAACAGCTGTATCCCCGTTGAGGGTGAGTTTTGCTTTTGTATCAGCCATTTAAGGTCTCCTTAGCGCCTTATTGCGTAAGACTGCCGGAACTTAAATTTGCCTTCGCACATCAACCTGGCTTTACCCGTTTTTTATTTGGCTCGCCGCTCTGTGAAAGAGG  >  NC_000913/754308‑754580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑