| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,296,380:1 | +CG | 100% | intergenic (+586/+56) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,380 | 1 | . | C | 95.3% | 98.2 / ‑1.5 | 42 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (23/17); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_000913 | 4,296,380 | 2 | . | G | 95.3% | 105.1 / ‑1.4 | 42 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (23/17); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCA > NC_000913/4296300‑4296507 || cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc > 1:538889/1‑143 (MQ=2) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGATTGATTGTGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc > 2:256691/1‑143 (MQ=11) tAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCAGACGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcca > 2:404174/1‑142 (MQ=11) aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGt > 1:612699/1‑143 (MQ=255) aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTGTTATCAGGCCTATCTTAACCGt > 2:709267/1‑143 (MQ=255) cgctTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg < 1:471652/143‑1 (MQ=255) gcgTCTTGTCCGGCCGAGCTCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg < 1:199084/143‑1 (MQ=11) gcgTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGGGTCGGGGCAGTA‑‑CA‑‑CCACCTACGCGAACAATTCCGGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg < 1:195355/143‑1 (MQ=9) ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtttac > 2:575910/1‑140 (MQ=255) tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAtttt < 1:596889/143‑1 (MQ=255) aGGCCTACGCCAGACAGCGCAATAGGCTGCATTAACGCCAGTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTAGTTAATTTTTc > 2:259714/1‑143 (MQ=14) gtcTACGCCGGGGAGCGCAATAGTCCGCTTTAGCGTGATCTTGCAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa < 2:673593/141‑1 (MQ=14) gCCTACGCCAGACTGCGCAATCGGCTTATTGAGCGTGATCTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa < 2:662178/143‑1 (MQ=14) gCCTACGCCAGACGGCGCAATAGCCTGATGTAGCGGGAGTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa > 2:103485/1‑143 (MQ=14) ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTCACTTTTTCaca > 2:260569/1‑141 (MQ=14) aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGCCATCAATGCCTGATGCGACGCTTGCCGCGTCTTTTCAGTCCGATCCTAACCTTTGGTTAATTTTTCACActt > 1:350923/1‑142 (MQ=14) aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAActc < 1:126330/143‑1 (MQ=255) gCCAGACTGGGCTATAGTCTGACTTAGCGTGAGTTTGTAGGTCGGAT‑ACGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCtt < 2:202683/143‑1 (MQ=17) cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTACTTTTTCACACTCTTca > 2:572369/1‑143 (MQ=14) cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCACACTCTTcc > 1:263961/1‑142 (MQ=255) aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCCATCTTAACCGTTGGTTAATTTTTCACAATCGTAACAccc > 2:796036/1‑143 (MQ=14) gCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 1:476996/143‑1 (MQ=255) gCCTGATTGAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTCTCaaa < 2:146381/143‑1 (MQ=18) cccTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 1:831333/142‑1 (MQ=255) ccTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc < 1:511885/143‑1 (MQ=255) cTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAAcc > 1:826145/1‑143 (MQ=255) cTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACGCCCGGTACAAAAcc > 2:458317/1‑143 (MQ=18) ttAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCACACCCTTcc > 1:51740/1‑143 (MQ=255) ttAGCGTGATTTTGGAGGTCGGAT‑AAGGC‑GTTGGCG‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTcc < 2:592547/143‑1 (MQ=21) tcgCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCCTCTTATCAGGCCTATCTTAGCCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa < 1:689129/141‑1 (MQ=21) tAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAACGCCTGAGGCAACGCTTGCCTCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa < 1:559386/143‑1 (MQ=18) aGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCAt < 1:436996/143‑1 (MQ=255) gCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGGTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTccttc > 1:208415/1‑140 (MQ=21) gCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa < 1:178042/143‑1 (MQ=255) cGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACGTCAATGCCTGATGCGACGCGTGCCGCGTCGTATCAGGCCCATCATAACCGATGGTTACTTTTTCAAACTCCTCAAATCCGGGATCAAGCTCTTCCATAc > 2:212452/1‑143 (MQ=17) cGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCCGTGGCTGAGTTTTCAAAATCTTCACACCCGGTATCAAACCCTTCCATAc > 1:525927/1‑143 (MQ=18) gTGATTTTGTAGGTCGGAT‑AAGGA‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATATTACACTGGCCGCGTTTGCTGGGGCCTATCTTAACCGTTGGTTCAGTTTTCAAACTCTTCACACCCGGTATCAAACCGTTCCATAcg > 2:178567/1‑142 (MQ=18) aTTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTGTCAAACCCTTCCATACAGCt < 1:508476/143‑1 (MQ=255) tAGGTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTCAATTCTTAAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAggct > 2:314230/1‑141 (MQ=21) gTCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTcga > 1:141462/1‑141 (MQ=255) tCGGAT‑AAGGC‑GTT‑‑TACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCGAc > 1:713315/1‑143 (MQ=255) ggAT‑TAGGT‑GTT‑‑TACGCCGCCTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCa < 2:306273/143‑1 (MQ=37) || CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGC‑GTT‑‑TA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCA > NC_000913/4296300‑4296507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |