| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,940,584 | C→A | 100% | R351L (CGT→CTT) | mepM ← | peptidoglycan DD‑endopeptidase MepM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,940,584 | 0 | C | A | 97.7% | 100.8 / ‑6.4 | 38 | R351L (CGT→CTT) | mepM | peptidoglycan DD‑endopeptidase MepM |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base A (18/17); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAG > NC_000913/1940446‑1940725 | tCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAAt < 1:780662/143‑1 (MQ=255) actGCAGACGCCGCCCGGTTGAACGCCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGATTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGcc < 1:177365/141‑1 (MQ=255) aGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGCAGAATAGcc > 1:447375/1‑143 (MQ=255) gTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGACGCCCCGTTTCACCTTCTGTCCCGGTTTCACCAGAATCATGCGCAAGTGCCTATAACGCGTGGCGCCGCTGCGACCATGCAGAACAGCca > 1:524561/1‑143 (MQ=255) gCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACAtaataa < 1:537707/143‑1 (MQ=255) cGGCCCGGGTGAACGCCCGGTATCACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAGGAAGAATAGCCACataagatc < 2:787867/143‑5 (MQ=255) gCCCGGTTGAACGGCCGGTCTTACCGGAAAGCGCGAGACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCGCATAATAACct < 1:558194/143‑1 (MQ=255) cccGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACctg < 2:120479/143‑1 (MQ=255) cGGTTGAACGTCCGGTATTACCGGAAAGCGCGCTACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAGTCTTGCGCAAGTGCATATAACGCGTGGCGTAGCTGCGACCATGACGAATAGCCACACAATAGCcggct > 2:527393/1‑143 (MQ=255) ggTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACctgctg < 1:446361/143‑1 (MQ=255) gAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGCTTCACCAGAATCTTGCGCAAGTGCATATAACTCGTGGTGTAGCTGCGACCATGAAGAATAGCACCAGAATCACCTGCCACGcc > 2:846627/1‑143 (MQ=255) aaCGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATATTTCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCa < 2:824160/143‑1 (MQ=255) gaaTTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATACAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGttt < 2:145239/141‑1 (MQ=255) ttACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGc < 1:616810/143‑1 (MQ=255) aCCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGCTGTAGCTGCGACCCTGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCaa > 1:479276/1‑143 (MQ=255) cgttACGTTCGCCACGGTTCACCTTCTGGCCCGTTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCCCTTCa < 2:743864/140‑1 (MQ=255) ccACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCAc < 1:196364/143‑1 (MQ=255) ttCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATACCGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTCGGCAACCACCACTTCACCGTCACCCACTGCAAg > 2:50270/1‑143 (MQ=255) ttCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGCAAg > 2:14457/1‑143 (MQ=255) tGTCCGGGTTTCCCCAGAAGCTTGCGCAAGTGCATATAGCGGGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGt < 2:835256/143‑1 (MQ=255) tCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGCCCATGACGAATAGCCACATCATAACCTGCTGCGCCACTGCGTTTGGCGACCACCGCTTCACCGTCACCCACGGACAGACCTGGCGTAc > 1:152030/1‑143 (MQ=255) cGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGCCGTACCCt > 2:793598/1‑143 (MQ=255) cACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCa > 1:22787/1‑143 (MQ=255) tCTTGCGCGAGGGCGTATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGaaa < 2:447375/143‑1 (MQ=255) ttGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATc > 1:79179/1‑143 (MQ=255) gcgcAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCaa < 2:744284/143‑1 (MQ=255) cAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAacac < 1:422754/143‑1 (MQ=255) cAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACAGTAACCCACTGATAGCACTGGCGTACCCTGCGGCATGGCGAAATCAacac < 1:803900/143‑1 (MQ=255) gTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGCAATCAACACctc > 1:80876/1‑143 (MQ=255) tataACGCGTGGTGTAGCTGCGACAATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGCTTGGCAACCACCACTACAACGTCACCCACTGAAAGCACTGGCCTACCCCGCGGCACGGCGCAATCACCACCTAcgtgt > 2:352807/1‑143 (MQ=255) taACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTgtgg > 1:728001/1‑143 (MQ=255) aCCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCCACGCGACCGGTCACCg > 2:796845/1‑143 (MQ=255) aCCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGGACCCTGCGGCATGACGAACTCACCCCCTCACCGTGGTCGAACGCGACCGGTCCCCg > 2:346925/1‑143 (MQ=255) ccATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCCCTTCACCGTCACCCACTGACAGCACTGGCGTACCCCCCGCCATGGCGCACCCAACACCTCTGTGCCGTGCAAAGCCACCGGTCAcctg > 1:806138/1‑141 (MQ=255) ccATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCCCTGCAAGCACTGGCGTACCCTGCGGCCTGGCGAAATCAACAACACACCGGCGCGCAACGCGACCGGTCCCCgg > 1:751947/1‑143 (MQ=255) aTGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAAACACCACTTCACAGACACCCACTGCAAGCACTGGCGTACCCTGCAGCATGAGGAAGTCAAAGCGGCCGCGTGGGGCAACGCGCCCGAGCACCggct > 2:390542/1‑141 (MQ=255) tGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGACAACCACCACTGCACCGTCACCCACTGAAAGCACTGGCGTACCCCGCGGCATGGCGAAATCAACACCCCCGTGCGGTGCAACGCGACCGGGCAcacgcac > 2:284756/1‑137 (MQ=255) aCGAATACCTACCTAACCTCCAGCTGCTCCACTTCGTTTGGCGACCACCACTTCACCGTCAGCCGGTGAAAGCGCTGCCTGACCCTGCGGCATGGCGAAATGAACGCCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAg < 2:337217/143‑1 (MQ=255) | TCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATTAG > NC_000913/1940446‑1940725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |