| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,941,326 | C→T | 100% | D104N (GAT→AAT) | mepM ← | peptidoglycan DD‑endopeptidase MepM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,941,326 | 0 | C | T | 100.0% | 69.5 / NA | 26 | D104N (GAT→AAT) | mepM | peptidoglycan DD‑endopeptidase MepM |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/11); total (15/11) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTC > NC_000913/1941192‑1941464 | ttCGCCGTCCGCGGATAATGACCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCAGCCGCAGCCAGTTGGGTGATATCACCCATAGCAATACCATACTGATTGCGAATGCTGCTTCGCGTATTGCCAGtcg > 2:192488/1‑141 (MQ=255) ggggccGCGGGTAATTTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCACTACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAaac < 1:709484/139‑1 (MQ=255) cgGTTAATGTCCAGGAGAGTTGTTGACCGATCTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTTGGGGAGATCACCCATATCAATACCATACTCAATGACAATGACACTTAGCGTATTACCAGTGGAaacaaactac > 2:796001/1‑138 (MQ=255) gTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTc < 1:196267/143‑1 (MQ=255) aCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGa > 2:17804/1‑143 (MQ=255) ccGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGAt > 1:527591/1‑143 (MQ=255) cGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCCCCGGCGAtt > 1:129070/1‑143 (MQ=255) cGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGACTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCAgcggcgggt > 2:230457/1‑140 (MQ=255) ttttCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCCTCACCGGCGATTTTg > 1:623556/1‑143 (MQ=255) tctCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCACTACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGt < 2:784435/141‑1 (MQ=255) ttACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCACTACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATGTTGTCATcccg > 1:459596/1‑141 (MQ=255) aCGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATGCAGtt > 1:465661/1‑143 (MQ=255) aCGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATACCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGGCATTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGtt < 1:758488/143‑1 (MQ=255) cAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATCCTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGt > 2:353927/1‑143 (MQ=255) tttGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAACCAACATATTCTTGCACGCCCGCTTCACCGGCGATTTTGTAATCCAGTATGTCATggg > 1:31551/1‑143 (MQ=255) gtgtgTGATATCACCCATCTCAATCCTCTACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATcttctt < 2:662493/139‑1 (MQ=255) atatCACCCATTGCGATTCCAGACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGc < 2:200256/143‑1 (MQ=255) actcATATCAATTCCAGCCTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGAt < 2:130674/140‑1 (MQ=255) atatCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCCTCTTCTTGTGCAGCTGGCTCAAt > 2:717511/1‑143 (MQ=255) aTACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTc > 1:378228/1‑143 (MQ=255) tACCATACTGAGTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCa < 2:137740/143‑1 (MQ=255) tGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTc > 1:558916/1‑143 (MQ=255) tGAGAATGCTGCTTAGCGTATTGCCAGTGGGAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGt < 2:93256/143‑1 (MQ=255) tgctgcGTAGCGTATTGCCAGTGGAAACAACATATTCGTGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGc < 2:159741/143‑1 (MQ=255) tggttAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGCTTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGcgaa < 2:185216/140‑1 (MQ=255) gTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTCCGTCGTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTATGAGCGAACGAATTTc > 1:757344/1‑143 (MQ=255) | TTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAGCGAACGAATTTC > NC_000913/1941192‑1941464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |