| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,956,975 | G→T | 100% | H11Q (CAC→CAA) | torZ ← | trimethylamine N‑oxide reductase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,956,975 | 0 | G | T | 97.1% | 91.9 / ‑4.7 | 34 | H11Q (CAC→CAA) | torZ | trimethylamine N‑oxide reductase 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (19/13); total (20/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTGTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTCCAGTTCGTTTTCGCTCATGCTGGTTCGTG > NC_000913/1956838‑1957117 | aCAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGCTGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGttt > 2:453382/1‑143 (MQ=255) ttCTTGCCGTCCTTCAGTTCTACGTTCATCGCTCCCCACCGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTtaat < 2:66563/142‑1 (MQ=255) aTCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGGTGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTtaat > 1:351310/1‑143 (MQ=255) aTCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCCATACCGCTTTGTTtcat > 1:544272/1‑143 (MQ=255) cTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCGCCACCGGCGCTCCAGCGGCAATCCCGGTTTGTTtcataa > 1:427928/1‑143 (MQ=255) gctgttcTTCACTTCTACGTTCATCGCTCCCGAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAAtt < 1:696846/137‑1 (MQ=255) tCCTTCACTTCTACGTTCTTCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACg < 2:132421/143‑1 (MQ=255) aCTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCTCCAGCGCTCCAGCGGCAATACCGCTCTGTCTACTAACTTCACgccctgc > 2:776155/1‑137 (MQ=255) gTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGAGGTCACCACCAGCGCTCCAGCGGCAATACCGCCTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCa > 2:683214/1‑143 (MQ=255) aTCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAAc < 1:772494/143‑1 (MQ=255) tCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTtcct > 2:469243/1‑143 (MQ=255) gccccAACGTCCCGCGGTGCGGATTTTACCGCCCGTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCCCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTtcct < 1:474448/142‑1 (MQ=255) cAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCt < 2:535204/143‑1 (MQ=255) cgGTGCGGATTTTACCGCCCTTCTCGTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAg < 2:812633/143‑1 (MQ=255) ggTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCACCGAgg < 2:702515/143‑1 (MQ=255) aGGATTCTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTCATAACTTCACGTCGTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCa < 1:356135/143‑1 (MQ=255) caTTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTGTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACt < 2:579246/142‑1 (MQ=255) tttACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCGACGGGGATCGCTGtt > 1:59595/1‑143 (MQ=255) cGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATCAATTCACGTCTTGTTACT‑GTCATCACTTCCTCCCTGCTCACCGCGGCTCACTGTTTCTCg > 1:148489/1‑143 (MQ=255) cccTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGCTCAACGAGGATCACTGTTTCTAGGt > 1:735007/1‑143 (MQ=255) ccTTCTCTTCTGTCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCCCCAGCGGCAATACCGCTTTGTATACTAAATTACAGTCTTGTTAAT‑GTCAAACCTTCATCCCCGATCAACGAGGATAACTGTTGCTAGGTa > 2:638121/1‑143 (MQ=255) ttctGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑ATCATAACTTCCTCCCTGATCAACGAGGATGACTGTTTATGGATAAGATcac > 2:428/1‑141 (MQ=255) ccATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGt < 1:207144/143‑1 (MQ=255) cGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTAc > 1:806251/1‑143 (MQ=255) ccGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGCACATACCGCGtt > 2:433183/1‑143 (MQ=255) gATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTGGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCCGTAATCTCTTTGGCGTTGTCCTGGAAATCACGCGTTaa > 1:436249/1‑143 (MQ=255) tCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAGAATg > 2:667893/1‑143 (MQ=255) accaccAGCGCCCCCGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTc < 1:522671/143‑1 (MQ=255) ccaccaGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATCTCTTTGCCGTTGTACTGGAAATACCGCGTTAAAATGTcc > 1:766766/1‑143 (MQ=255) caGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATCACTTCCGCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTCCAGtt < 1:388681/143‑1 (MQ=255) ccAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTGATATCTTTGGCGTTGTACTGGAAGTACCGCGTTAAAAGGTACAGTTCGTTTTc > 1:363009/1‑143 (MQ=255) ggCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTCCAGTTCGTTTTCGCTCa < 1:743228/143‑1 (MQ=255) gCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTACAATGACCAGTACGTTTTccaccag > 2:285588/1‑137 (MQ=255) cGCTTTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCCCGGTAATATCTTTGGCGTGGTACTGGAAATACCGCGTTCACATGTCAAGTTCGTTTTCGCTCATGCTGGtt > 2:393858/1‑143 (MQ=255) gtgtTTACTCAGTTCACGCCTTGTTAATCGTC‑TAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTCCAGTTCGTTTTCGCTCATGCTGGTTCGTg < 2:436249/143‑1 (MQ=255) | ACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTGTGTTTAATAAATTCACGTCTTGTTAAT‑GTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTCCAGTTCGTTTTCGCTCATGCTGGTTCGTG > NC_000913/1956838‑1957117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |