| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 200,642 | C→T | 11.6% | A54V (GCC→GTC) | skp → | periplasmic chaperone Skp |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 200,642 | 0 | C | T | 11.6% | 69.7 / 2.4 | 35 | A54V (GCC→GTC) | skp | periplasmic chaperone Skp |
| Reads supporting (aligned to +/- strand): ref base C (17/13); new base T (2/2); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
GCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCT‑GACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAGCGCCAGACTTTTGCTCAGAAAGC > NC_000913/200500‑200774 | gCTGCAGGTCTCGGTTTAGCTCTGGCAACTTCTGCTCAGGCGGCT‑GACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGc < 1:309283/143‑1 (MQ=255) ggCAGGTCTCGGTCTAGCACTGGCAACTTCTGCTCAGGCGGCT‑GACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCa < 2:350489/142‑1 (MQ=255) tctcGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCT‑GACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGAGCCGAACACCGGTGTCTCTAACACGCTGGAAACGGAGCTCAAGGGCCGTGCCGGCGacc > 2:508502/1‑141 (MQ=255) tcGGTGTAGCACTGGCGTCTTCTGGTCAGGCGGCT‑GACAAGATGGCGATCGGCGACATGTGCAGGCTGGTCCGGCAGGGAGCGCAGAAAACCGGTGTTGCTAACACGGTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTg < 2:407963/143‑1 (MQ=255) tttAGCGCTGGCAACCTCTGCGCAGGCGGCT‑GACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAACCCGGTGTTTCTAACACGCTGGCAAATGATTTCAATGGCCGTGCCAGCGAACTGCAGc < 2:61543/143‑1 (MQ=255) cACTGGCAACTTCTGCTCAGGCTGCTCCGCAGAAGT‑CAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAAGGAGTTCAAAGGCCGTGCCAGCGAACCGCCGCGTATg > 2:604059/1‑143 (MQ=255) tGGCAACTTCTGCTCAGGCGGCT‑GACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTAGGGaa > 1:244172/1‑143 (MQ=255) cTTGTGCTCAGGCGGCT‑GACAAAAGTGCAATCGTCAACATGGGCAGCCTGGTCCAGCAGGTAGCGCAGAAAACCGGTGTTGCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGAt < 1:297654/143‑1 (MQ=255) cTCAGGCGGCT‑GACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAg > 1:490362/1‑143 (MQ=255) cggcggcG‑TACAAAAGTGCAATCGTCAGCATGGGCAGCCTGTTCCAGTAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAGGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCt < 2:39814/142‑1 (MQ=255) gcggcT‑GACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGTGAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTaa > 1:23009/1‑143 (MQ=255) gcggcT‑GACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGTCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTac > 1:23057/1‑142 (MQ=255) aaaaTTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGaaaaa > 1:322351/1‑143 (MQ=255) aaaaTTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTATCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACGAATCTGGAGGCTGAAAAGaaaaa > 1:777355/1‑143 (MQ=255) ttGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTg < 2:471052/143‑1 (MQ=255) ttGCAATCGTCAACATGGGCAGCCTGGTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTg < 1:278278/143‑1 (MQ=255) tCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCCGTCCagg > 2:283677/1‑141 (MQ=255) cAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTCAAATGAAAACGCTGCAGTCCATCACAGCGGGCa > 2:530014/1‑143 (MQ=255) cAGCCTGTACCAGCAGGGAGCGCAGAAAACCGGTGTTTCTAAAACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCa < 1:455514/143‑1 (MQ=255) tGTTCTAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGTCAGCGAt < 1:499431/143‑1 (MQ=255) gTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCAAAAATGAAAAAGATGCAGGCCAAGGAGGGGGGGAGCGacc > 2:4643/1‑141 (MQ=255) ccAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGAACTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCa > 2:353364/1‑143 (MQ=255) agGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAg > 1:362238/1‑143 (MQ=255) gTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCt > 1:693637/1‑143 (MQ=255) aGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTgg < 1:47973/143‑1 (MQ=255) cgcAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCACGAAAGCGGGCAGCGATCGCACTACCCTGGaa > 2:410541/1‑143 (MQ=255) tAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCTATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAgc < 1:235978/143‑1 (MQ=255) acGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAGCGCCa < 1:698496/143‑1 (MQ=255) ggtGGAAAATGAGTTCAAAGGCCGTGTCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAGCGCCAGa < 2:23057/141‑1 (MQ=255) gCTGGAAAATGAGTTCAAAGGCCGTGTCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAGCGCCAGa < 2:23009/143‑1 (MQ=255) ggAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAGCGCCAGACtt > 1:366084/1‑143 (MQ=255) gAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCAATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAACAGGCGGGATGACTCAGCGCCCGACttt > 2:277265/1‑143 (MQ=255) gAAAATGAGTTCAAAGGCCGTGACAGCGAACTGCAGCGTAAGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGAACTAAGCAGGAAGAAGACGTGAGGGCTCGACGCCAGagctt > 2:656193/1‑139 (MQ=255) gTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAACTGAAAACGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTCAGCTGGAAAAAGACGGGATGGCTCAGCGCCAGGCGTTTGCTCAGa > 1:562315/1‑143 (MQ=255) gcagcggCCGTGCCAGCGAACGGCAGCGTATGGAAGCCGATCTGCAGGCGAAAATGAAGAAGCTGCAGTCCATGAAAGCCGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAGCGCCAGACTTTTGCTCAGaaa < 2:650130/138‑1 (MQ=255) aaaGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTGCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATGGCTGAGCGCCAGCCTTTTGCTCAGcaacg > 1:79790/1‑141 (MQ=255) | GCTGCAGGTCTCGGTTTAGCACTGGCAACTTCTGCTCAGGCGGCT‑GACAAAATTGCAATCGTCAACATGGGCAGCCTGTTCCAGCAGGTAGCGCAGAAAACCGGTGTTTCTAACACGCTGGAAAATGAGTTCAAAGGCCGTGCCAGCGAACTGCAGCGTATGGAAACCGATCTGCAGGCTAAAATGAAAAAGCTGCAGTCCATGAAAGCGGGCAGCGATCGCACTAAGCTGGAAAAAGACGTGATGGCTCAGCGCCAGACTTTTGCTCAGAAAGC > NC_000913/200500‑200774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |