| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,306,277 | A→G | 18.7% | L160L (TTG→CTG) | mqo ← | malate:quinone oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,306,277 | 0 | A | G | 18.7% | 69.0 / 5.1 | 38 | L160L (TTG→CTG) | mqo | malate:quinone oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (12/17); new base G (5/3); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCACATCGGTACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAA > NC_000913/2306136‑2306413 | tCACATCGCTACCAATGTCCGGACGCGTGGCCGCCACGTTCTGTCGCGGATCGCGCCCTTCCATCACTCACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAAc < 1:584715/143‑1 (MQ=255) tCGGTAGCCGTTTCCGTCCGCGCGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAGCGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACgcggc < 2:167765/143‑1 (MQ=255) gTACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCCGATCGCGCCCTTCCATCCCTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCACAATAACGCATACCCCCAACCAGCGAACCTTATTCAAACgcggctaa > 1:784301/1‑140 (MQ=255) tccctagttCCGTACGCGTGGCTGCCACTTTCTGTGGCGGATCGCGCCCTTCCATCACTAGCGGTGCCCACGCTTTGATCTCCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTaa < 2:735027/136‑1 (MQ=255) tACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAGCGCGGCGTaa > 1:73197/1‑143 (MQ=255) tACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAGCGCGGCGTaa > 1:469624/1‑143 (MQ=255) tACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTaa > 1:398947/1‑143 (MQ=255) tACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATAGCGCCCTTCCATCACTAACGGTGCCCGCTCTTTGATCCGCGCGCGCTACTAAGAGTAACGGCTACGCGGCAAAAGCCGGCCTTGTTGCAGCgcggctcaa > 2:353357/1‑139 (MQ=255) aCCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTATCGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAAc < 2:838138/143‑1 (MQ=255) cAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATCCCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACgg > 1:681924/1‑143 (MQ=255) gtttCCGGACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGgc < 1:747320/142‑1 (MQ=255) aTTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTGACGGgc > 1:60481/1‑143 (MQ=255) aTTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGgc < 1:197078/143‑1 (MQ=255) tCCGTACGCGTGGGTGCCACTTTCTGTTGCGGGTTTCGCCCTTCCCTCACTAACCGTGCCCACCCTTTGATCTGCGCGTGATCTTCAGAGTAACGCCTACCGCGGAACAGCGGGCGTGTGTGCAGCGAGGCGGAACCGgcgcg > 2:649650/1‑143 (MQ=255) gTACGCGTGGCTGCCACTTTCTGTAGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCAGCGCGTGATCTTCAGAGTAACGCATACCCAGCAACAGCGAGCTTTGTTGTAACGCGGCGTAACGGGCGCGCAg > 2:842538/1‑143 (MQ=255) cGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCAAGa > 2:227999/1‑143 (MQ=255) aTCGCGCTCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACaa < 2:813369/143‑1 (MQ=255) ttCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGGGTAACGCATACCGCGAAACAGCGAGCATTGTTGCCACCCGGGCTAACGGGCGCGCAGGCAATTGACGCTATACTCGCCCCAGACGAAGCTCctat > 1:777594/1‑143 (MQ=255) tCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCGGCGCGGCGTAACGGGCGCGCAGGAACTTGACGTTATCCTCGCCCCAGACAAAGCTCGTATg > 2:97080/1‑143 (MQ=255) ggTGCCCACTCTTTGATCTGCAAGTGAGCGTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTAtt < 1:114542/143‑1 (MQ=255) ccACTCTTTGATCTGCGCGTGATCTTCGGTGTAACGCATACCGCGAATCCGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATaa < 1:793197/143‑1 (MQ=255) taTCTGCGCGTGATCTGCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAAcgcg < 1:345425/142‑1 (MQ=255) gATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAGCGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAAcgcg < 2:469624/143‑1 (MQ=255) gATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAGCGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAAcgcg < 2:73197/143‑1 (MQ=255) tCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCACCGCGGCGTACCGGGCGCGCCGCAAATCGACGTTCTTCTTCCCCCAGACAAACCTCATATGCGGCACCGTATTGATACCAGAACGCggc > 1:430312/1‑142 (MQ=255) tGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGt < 2:153756/143‑1 (MQ=255) gcgcGTGTTCCTCATAGTAACGCATACCGCGAAACAGCGAGCTTTGTGGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTa < 1:332337/143‑1 (MQ=255) tGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGACCGGTATTGATAAATGACCGCGGAGTACgccg > 2:151062/1‑141 (MQ=255) cATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGCACGGTATTGATAAATGAACCCGGGGTACGCAGCACGCCGCGCTCAACCt > 2:337920/1‑143 (MQ=255) aTACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTg > 2:743420/1‑143 (MQ=255) cgcgAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTgc > 1:823363/1‑143 (MQ=255) aaaCAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGCAACGGTCTTGAAAAATGAGTGCGGAGTACGTAGCACGCCGCGCTCAACCTGGTGCGccc < 1:35601/143‑1 (MQ=255) gcAGCGAGCTTTGTTGCAGCGCGGCGTAACGGGCGCGCAGGAAATTGACGTTAGCCTCGCCCCAGACGAAGCTCATATGCGGAACGGTATTGATAACTGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAg < 2:428683/142‑1 (MQ=255) aCAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAg < 1:165232/143‑1 (MQ=255) aGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGaa < 2:550016/143‑1 (MQ=255) cGAGCGTTGTTGCAACGCGGCGTATCGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACt < 2:398947/143‑1 (MQ=255) aGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTgg > 1:465421/1‑143 (MQ=255) gtggCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGaa < 1:75992/140‑1 (MQ=255) | TCACATCGGTACCAATTTCCGTACGCGTGGCTGCCACTTTCTGTTGCGGATCGCGCCCTTCCATCACTAACGGTGCCCACTCTTTGATCTGCGCGTGATCTTCAGAGTAACGCATACCGCGAAACAGCGAGCTTTGTTGCAACGCGGCGTAACGGGCGCGCAGGAAATTGACGTTATCCTCGCCCCAGACAAAGCTCATATGCGGAACGGTATTGATAAATGAACGCGGAGTACGCAGCACGCCGCGCTCAACCTGGTGCGCCCAGAACTGGCGGGAA > NC_000913/2306136‑2306413 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |