| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,750,073 | G→T | 10.2% | intergenic (+13/+42) | yfjD → / ← grpE | putative inner membrane protein/nucleotide exchange factor GrpE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,750,073 | 0 | G | T | 10.2% | 71.7 / 2.3 | 38 | intergenic (+13/+42) | yfjD/grpE | putative inner membrane protein/nucleotide exchange factor GrpE |
| Reads supporting (aligned to +/- strand): ref base G (13/19); new base T (2/2); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01 | |||||||||||
CCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCA > NC_000913/2749935‑2750214 | ccTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGCATCCGCTTGCCGAGAGTGTGGCGGAGTAACGAAAACGGCCACGCAt > 1:21039/1‑143 (MQ=255)ccTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCAt > 1:232586/1‑143 (MQ=255) gggaCCGGCGGGCGTGTGGGAGAGTACGACATCGATACTCTCGACTGACAGGACAATATGATTAAGCAGGTAGACGTTTTTCCTGTGCAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCa < 2:360074/140‑1 (MQ=255) gtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTGTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTACCGAAAACGGACCGGCAATCGCATGCAGGGCCCTGa > 2:401340/1‑143 (MQ=255) attctcGAGTAAGATAAGGATATCCGGGACGTACAGGACAATAGGAATAAGCAGGAAAAAGCGTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAattatt < 1:186552/138‑1 (MQ=255) gtGGCGAGTACGAGATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGATGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAAttatta < 1:53796/142‑1 (MQ=255) cGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGaa > 1:498083/1‑143 (MQ=255) tACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCGGGGCCGGGAATTATTACGAAACCa > 1:700894/1‑143 (MQ=255) atagcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGaaa < 1:242443/143‑1 (MQ=255) tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGGATGATTACGAAAGCAGAAAt > 2:734181/1‑143 (MQ=255) cgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTaa < 2:670398/143‑1 (MQ=255) tatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATTCGCATGCAGGGCCGTGAATTATTCCGAAAGCAGAAATTAAGCt > 1:700892/1‑143 (MQ=255) tctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTCCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCtttt < 1:707601/143‑1 (MQ=255) ctcGACGTCCAGCACACTATGATTAAGCAGGTAACAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTACCGAAAACGGCCCGACATTCGCATGCAGGGCCGTGAGCTACTACGAAAGCAGAAACTAAGCTGTTg > 2:833774/1‑143 (MQ=255) tgtaggTACAGGACAATATGATTAGGCAGGTAAAAGTTGTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGc < 2:587973/138‑1 (MQ=255) cagggaaaTATGCTTAACAAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTa < 2:329066/137‑1 (MQ=255) tggcAATATGAGTAAGCAGGTACGAGTTTTTCTTGTGAGACCGCTGCGCGAGAGTGTGGCGGAGTAACTAAAACGGCCCGGTATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACa < 2:474895/140‑1 (MQ=255) gACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGCGTAACGAGCAAGGCCCAGCATTCGCACGCAGGGCCGTGAATTATTACGAAAGCAGCAATTAAGCTTTTGCTTTCGCTACAg > 1:390210/1‑143 (MQ=255) aaTATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTaa < 2:521909/143‑1 (MQ=255) atagGATTAAGCAGGGAAAAGTTTTTCCGGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAAc < 2:155934/143‑1 (MQ=255) atctaGCAGGTAAATGTTGTTCCTGGTAAACCGCTGCGCGAGAGTGTGGGTGAGTCACGAAAACGGCCCTGCAGTCGCATGCAGGGCCGTGAATTATTACTAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcg < 2:231473/139‑1 (MQ=255) aTTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACCGTAACCATcg > 1:293068/1‑143 (MQ=255) ttAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgc > 2:299037/1‑143 (MQ=255) aGTTTTTCCTGTGAAGCCGCTGCGCGAGAGTGGGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTa < 2:50856/143‑1 (MQ=255) ttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGCATCGTACGa > 1:183566/1‑143 (MQ=255) aaCCGCTGCGACATGATGTTGCGGTGTAACTAACATGGCCCGGCATTCGCATGCAGGGCCTTGAATTATTACGAAAGCATAAATTTAGCTTTTGCTTTCGTTACAGTAACCATCGCCGCACGAATCTTACGACCATTCAGCGt < 2:837779/143‑1 (MQ=255) aCCGCTGCGCGAGCGGGTGGCGGAGTAACGAAAACGGCCCGCCATTCGCATGCAGCGCCGCGACTTATTACGAAAGCAGAAATTAAGCTCTTGCTTTCGCTACAGGAACCATCCCCACACGAATCGTACGACCAGTCAGcaga > 2:424851/1‑140 (MQ=255) aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACCGTAACCATCGCCGCACGACTCGTACGACCATTCAGCGtc > 2:317423/1‑142 (MQ=255) aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGta > 2:572527/1‑143 (MQ=255) cTGCGCGCGAGTCTGGCGTAGTAACGAAAACGGCCCGGCAGTCGCATGCAGGGCCGTGAATTATTACGAACGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAc < 1:8000/143‑1 (MQ=255) gagaGTGTGCCGGAGTAACGAACACGGCCCGGCATTCGCATGCAGGGCCGTGAATTCTTTCGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCt < 2:786235/143‑1 (MQ=255) tgtgTCTGAGTAACGAAAACGGCCCGGCATTCACATGCAGGGCCGTGAATTAGTACGAAAGCAGAAATTCCGCCCTCGCTTTCGCTACAGTCACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATa < 1:641853/143‑1 (MQ=255) cGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGcc < 1:822625/143‑1 (MQ=255) gAGTAACGAAAACGGCCCTGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCa < 2:700892/143‑1 (MQ=255) tAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTa < 2:232586/143‑1 (MQ=255) aattcaCGGCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGt > 1:418000/6‑143 (MQ=255) ccGGCCGGGCATTCCCATGCAGGGCCGTGAATTATCACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCt < 1:110484/142‑1 (MQ=255) cGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCa > 1:494999/1‑143 (MQ=255) | CCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCA > NC_000913/2749935‑2750214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |