Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,398	T→G	100%	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	139.0 / NA	47	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/26);  total (21/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTC  >  NC_000913/3041261‑3041535                                                                                                                                          |                                                                                                                                          gCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTGTTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGcca                                                                                                                                      <  1:211954/143‑1 (MQ=255) gCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGcca                                                                                                                                      >  1:595518/1‑143 (MQ=255)  ctctTTGACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTGTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccac                                                                                                                                     <  2:243361/143‑1 (MQ=255)             ttAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCACGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCCCCATTGCTGgcg                                                                                                                          >  1:80272/1‑143 (MQ=255)               aGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGcgcg                                                                                                                        <  1:549244/143‑1 (MQ=255)                 tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa                                                                                                                      <  1:539679/143‑1 (MQ=255)                   aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACa                                                                                                                    >  1:137239/1‑143 (MQ=255)                   aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGACTTTTACACCTTTTACACACAGTCAGCCTACGGCTTCTTTACGTTTACCGATGACGCTAATGACGCTTGAGTACTGGACGCGTGCCACCCAGAGTGGCGCGGACa                                                                                                                    >  2:509207/1‑143 (MQ=255)                     tAATACGCCGTTAAGCATCTCGGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGc                                                                                                                  <  1:428474/143‑1 (MQ=255)                        tACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCGCCATTACTGGCGCGGACAGCGaa                                                                                                               >  1:699316/1‑143 (MQ=255)                        tACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCgca                                                                                                               >  1:568257/1‑141 (MQ=255)                         aCGCCGTTAAGCAACGCAGGACGCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaaa                                                                                                              <  2:521140/143‑1 (MQ=255)                              gtcacgCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACGGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatat                                                                                                         <  1:196203/138‑1 (MQ=255)                                tAATCAACTCGGGACTCTATTATCGTTTTTACACCTTCTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatatat                                                                                                       <  2:492311/143‑1 (MQ=255)                                        tCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTACCACCATGACTGGCGCGGACAGCGAAAACTATATGCAGGGTc                                                                                               >  1:427375/1‑143 (MQ=255)                                          acgtctctATTATGGCTTTTACACCTTTCCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAg                                                                                             <  2:62889/139‑1 (MQ=255)                                           ggACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgg                                                                                            <  1:126092/143‑1 (MQ=255)                                             aCTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCTTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTg                                                                                          >  1:439941/1‑143 (MQ=255)                                              ctctATTATGGCTGTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGa                                                                                         <  1:72173/143‑1 (MQ=255)                                                   ttatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGGCCGGTGAcagca                                                                                    >  1:302046/1‑143 (MQ=255)                                                    tatGGCTTTTACACCTCTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCAGACGCAGACGACCCTTGATGACCGGCCGCGTGCCCCCCCTACTGGACAAGACAAAGAAAAATATATGCAGGGTCAGGTGAcagcag                                                                                   <  1:376348/143‑1 (MQ=255)                                                     atGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGACCGTCGACCGCTGACGCTGATGACGCTTGATGCCTGCGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAGATATATGCACGGTCACGTACCACCAGa                                                                                  >  2:389172/1‑143 (MQ=255)                                                     atGGCTTTTACACCCTTTCCTCCCCGTCAGCCTACGGCTTCAAACGCTACACGTGGGACGCTGATGCCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGa                                                                                  <  1:353296/143‑1 (MQ=255)                                                      tGGCTTTTACACCTTTTCCCCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGAt                                                                                 <  1:342420/143‑1 (MQ=255)                                                                  cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATg                                                                     >  1:362267/1‑143 (MQ=255)                                                                     ttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAGAATATATGCAAGGGCAGGTGACAGCAGGTGTCAGCCAGATGGcc                                                                  >  1:770653/1‑142 (MQ=255)                                                                     ttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCa                                                                  <  1:567614/143‑1 (MQ=255)                                                                          ccccGGCAGCCTCCGGCTTCTGCCCGCTTGCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaaga                                                             <  1:823622/143‑1 (MQ=255)                                                                             cGTCAGCCTCAGGCGTCTGCCCGTTTACCGCTGGCGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCa                                                          <  2:478952/143‑1 (MQ=255)                                                                             cGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCa                                                          <  1:203517/143‑1 (MQ=255)                                                                                   ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCTTGATGACTGGGCGCGTGCCACAATTACGGGCGCGGACAGCGAAAACAATATGAAGGGGCAGGGGACGGCAGAAGGCAGCAAGCTGGCAGGGGACCACCACCt                                                    >  2:749892/1‑143 (MQ=255)                                                                                      aCGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGACAGATGGCAGAAGATCAGCACATGCt                                                 >  2:495897/1‑143 (MQ=255)                                                                                         gCTTCTGCCCGTTTCCCGCTGACGCCGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgc                                              <  2:234909/143‑1 (MQ=255)                                                                                            tCTGCCCGTTTACCGCTGACGCTGATGACGCTTGCTGAGTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccgc                                           <  1:267678/143‑1 (MQ=255)                                                                                               gCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATAAGCACCGGCTCGCCGCCCa                                        >  2:707151/1‑143 (MQ=255)                                                                                                cccGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGCAGATCAGCACCTGCTCGCCGCCGAt                                       >  2:380792/1‑143 (MQ=255)                                                                                                 ccGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCAtt                                      <  2:746246/143‑1 (MQ=255)                                                                                                          gctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCACGCTGCCCATTGCGACGCCa                             >  2:691876/1‑143 (MQ=255)                                                                                                               cgctgaTGCCTCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCCGTCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGt                        <  2:377283/143‑1 (MQ=255)                                                                                                                    atgaCGCTTGATGACTGGGCGCGTGCCACCAGTACTGGCGCGGACAGCAAAAATTAGAGTCTGGTTCCGGTAACATCAGATGTCAGCCAGATGGCAGAAGACCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAggcagact                   >  2:178391/1‑137 (MQ=255)                                                                                                                      gaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtgt                 >  2:349808/1‑143 (MQ=255)                                                                                                                        cGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTgg               <  1:661203/143‑1 (MQ=255)                                                                                                                           ttGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACGGACGATGTAGGCAAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGGCGCGAAAGGTAAAATGTGGAGc            >  1:143044/1‑143 (MQ=255)                                                                                                                              atgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAt         <  1:85881/143‑1 (MQ=255)                                                                                                                                 aCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAGAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTa      <  2:520531/143‑1 (MQ=255)                                                                                                                                 aCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGGCAGCAGATGTCAGCCAGATGGCAGTAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTa      <  2:416554/143‑1 (MQ=255)                                                                                                                                     ggCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGGGGAGCAATTTACGTc  >  2:520391/1‑143 (MQ=255)                                                                                                                                          |                                                                                                                                          GCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTC  >  NC_000913/3041261‑3041535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑