| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,486,157 | A→C | 100% | E13A (GAA→GCA) | crp → | DNA‑binding transcriptional dual regulator CRP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,486,157 | 0 | A | C | 97.5% | 112.3 / ‑5.2 | 38 | E13A (GAA→GCA) | crp | DNA‑binding transcriptional dual regulator CRP |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (17/20); minor base T (1/0); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGG > NC_000913/3486026‑3486289 | ccccTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGGAAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTCGGCAAACGGCAAACAGAACCGACTATCGCATGGTTCTTGt > 1:375784/1‑143 (MQ=255) ccTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGTTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGtct < 1:58755/143‑1 (MQ=255) cTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTCGGCAAACCGCAAACAGACCCGACCCTCGCATGGTTCTTGtctc < 1:559952/143‑1 (MQ=255) tCCCAGGGAGCGGTAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCAt < 2:162098/143‑1 (MQ=255) cGGGAAGCATATCTCGGCAATCCAGAGACAGCGGCGTTAGCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTc < 1:121965/143‑1 (MQ=255) gggAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTGATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCa < 2:87326/143‑1 (MQ=255) gCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTGTCGCATGGTTGTTGTCTCATTGCCACAttgcgtagc > 2:476545/1‑136 (MQ=255) ttCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCAt > 1:21195/1‑143 (MQ=255) cGGCAATCCAGAGACAGCGGCGCGATCTGGCTCGGGAGAAAGCGTATAACAGAGGATAACAGCGCATGGGGCTTGGCAAACCGCAAAAAGAACCGACACACACATGGGACTTGCCTCATTGAAACAGACAACAGTAGCCATcg > 2:75154/1‑142 (MQ=255) aaTCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAATCCGCATCCAGACTGGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAaga < 2:824153/143‑1 (MQ=255) agagACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCACGAGCACg > 1:393326/1‑143 (MQ=255) cAGCGGCGTTATCTGGGTCTGGTGAATGCTCATAACAGATGATAACCGCGCATGGTGCTTGGCAAACCTCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCAGAAGTACCCATCCAAGAGCACGCTTAt < 2:51484/143‑1 (MQ=255) ggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCGTTGCCACATTCATAAGTACCCCTCCAAGAGACCGCTTATTCag > 2:527582/1‑142 (MQ=255) cgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCa < 1:772249/143‑1 (MQ=255) ttATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGGTTATTCAACAgg > 1:432574/1‑143 (MQ=255) aTCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGt < 1:670642/143‑1 (MQ=255) ctGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGc < 1:774808/143‑1 (MQ=255) agaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGaaaagtcggca > 1:303418/1‑136 (MQ=255) aaaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCCCGCTTATTCACCAGGGGGAAAAAGCGGAAAc > 2:592140/1‑143 (MQ=255) cgtctgaCAGGGGAGAACCGCGGATGGTGCTTGTCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACTCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg < 2:427341/137‑1 (MQ=255) cTTATGACGGAGGATAACGGCGCATGGGGCTTGGCAAACCGCAACCAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACAGTCAGAAGTACCCAGCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTg < 2:288051/143‑1 (MQ=255) ttATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGt > 1:755113/1‑143 (MQ=255) tataACAGAGGATAAGCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGta < 1:494993/143‑1 (MQ=255) tataACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGta < 1:539503/143‑1 (MQ=255) ataACAGAGTATACCCTCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAACAGCGGGATTGCTGttc < 2:145664/143‑3 (MQ=255) aCCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATACGTACCCAGCCGCGGGCACGCTCATTCACCAGGGTGCAAACGCGGCCCCGCTGTACTACCTCGTTAAAg > 1:115487/1‑143 (MQ=255) aTGGTGCTGTGCAAACCGCAAACAGGCCCGACTCTCGCATGGGTCTTGTCTCATTGTCACATTCGTAAGTGCCCAGCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgt < 2:757676/143‑1 (MQ=255) tGGTGCTTGGCAAACCGCAAAAAGACTTATCTCTCTTGGTGTTCTTTTCTCATTGCCACATTCATAAGTAGCCATCCAAGAGCAAGCCTATTCACCAGGGTGAAGAAGAGGAAAAGCTGTACTACATGGTTAAAGCCGCtgtg > 2:62182/1‑143 (MQ=255) ggCTTGGCAAGCCGCAAGCAGACCCGGCTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCa < 2:812103/142‑1 (MQ=255) cTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCCCGCGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACGACGTCGTTAAGGGCCCGTTGGCAgg > 2:289534/1‑142 (MQ=255) cTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGGTCAAGGCGCggtgcccgt > 1:695149/1‑138 (MQ=255) ggCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCt > 1:147052/1‑143 (MQ=255) gCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCCCATTGCCACATTCATAAGTACCCATCCACGAGCACGCTTATTCCACAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTGAAAGGCGCGGCGGAAGTGccg > 2:606358/1‑141 (MQ=255) cGCAACCGGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCaaa < 1:754271/143‑1 (MQ=255) cAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAACAAGCGGAAACGCTGTACTACATCGTTAAAGGCCCTGTGGCAGTGCTGATCAAAGa > 2:27183/1‑143 (MQ=255) aCAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGa < 1:265848/143‑1 (MQ=255) cctaCCCGACTCTCGCAGGGTTCGTGTCTCATTGCCACCTTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGaa < 2:444678/140‑1 (MQ=255) ccGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAggg > 1:425117/1‑143 (MQ=255) | CCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGG > NC_000913/3486026‑3486289 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |