Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,515 T→A 5.9% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase (NADP(+))/peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,5150TA 5.9% 281.7 / 7.1 99intergenic (+70/+17)ilvC/ppiCketol‑acid reductoisomerase (NADP(+))/peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand):  ref base T (48/44);  new base A (3/3);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-01

Only 100 of 105 total aligned reads displayed.

TGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAA  >  NC_000913/3959373‑3959657
                                                                                                                                              |                                                                                                                                              
tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                                                <  1:90229/143‑1 (MQ=255)
tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                                                <  1:227789/143‑1 (MQ=255)
tGCGATTAAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCATAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                                                <  2:382088/143‑1 (MQ=255)
   gATTGAGCGGGTAGGGAAGAAACTGCGCGGCTATATGACAGATATGAAGCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                                                             <  2:111447/143‑1 (MQ=255)
       taGCAGGCAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAg                                                                                                                                         <  1:498156/142‑1 (MQ=255)
       gtgcgggtaggtaAGAAATTGCGCGGCTACATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAg                                                                                                                                         <  2:217017/138‑1 (MQ=255)
       gAGCAGGTAGGTAAGAAACTGCGCGGCGCTATGACAGATAGGAAACGTATTGCTGTGGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCTGGAGAAg                                                                                                                                         <  2:218485/143‑1 (MQ=38)
        aGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg                                                                                                                                        <  2:673881/143‑1 (MQ=255)
          gaggtagggCAGAAACGGCGCGGCTATATGACAGAGAGGAAACGTAGGGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCAGGAGAAGGcc                                                                                                                                      <  1:709126/142‑1 (MQ=25)
           aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                                                     <  1:829377/143‑1 (MQ=255)
              taggtaCGAAACGGCGCGGCTATAGTACAGATATGAAACGTATTGCTGTTGCGGGTTAAGCGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGc                                                                                                                                  <  2:436957/143‑1 (MQ=38)
                 gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGGTAt                                                                                                                               >  1:2246/1‑143 (MQ=255)
                 gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGGTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAt                                                                                                                               <  2:351638/143‑1 (MQ=255)
                 cgaaGAAACTGCGCGGCTATGTGACAGATATGGAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAt                                                                                                                               <  2:106626/141‑1 (MQ=255)
                   aaGAAACTGCGCGGCTATATGACAGATATGAAACGTAGTGCTGTTGAGGGTTAAGTGAGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACGCTCAAGGCCTTCTCCTGGAGAGGGCCTTGCTATTa                                                                                                                             >  1:760580/1‑143 (MQ=38)
                   aaGAAACGGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCACACAGGGAGAGGGAGAAAGCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                                                                                             <  2:601183/143‑1 (MQ=255)
                    aGAAACTGCGCGGCTATATGACAGATATGAAACGTATGGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAg                                                                                                                            <  1:501763/143‑1 (MQ=255)
                      aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGGCCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGtt                                                                                                                          >  1:497894/1‑143 (MQ=255)
                      aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGGTAAGTGCGCGCTGATGCCCTCACCGCGACCCACTCCCACAGGGAGAGGGAGACAACACACAAGGCCTTCTCCTGGCAAAGCCCTTGCTATTAggg                                                                                                                          >  2:452055/1‑141 (MQ=37)
                        aCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGc                                                                                                                        >  1:733404/1‑143 (MQ=255)
                         cTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                                                                                       <  1:836734/143‑1 (MQ=255)
                          tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCATTGCTATTAGTTGCgg                                                                                                                      >  1:22242/1‑143 (MQ=255)
                          tGCGCGGATAGATGACAGATATGATACGTATTGCTGTTGCGGGTTAAGGGCGCGCTGATGCCCTCACCCCGACCCTCTCGCACAGGGAGAGGTATAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                                                                      <  2:384136/143‑1 (MQ=38)
                            cgcgGCTGTATGGCTGATATGATACGTATTGCTGTGGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTa                                                                                                                    <  2:657763/143‑1 (MQ=38)
                              tggCTATATGACAGTTATGAAACGTATGGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTACCCAACACGAAGAGGGAGTAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                                                                                  <  1:230045/142‑1 (MQ=255)
                              cgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGGGGGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAGTAGTTGGGGTACa                                                                                                                  >  1:499499/1‑143 (MQ=255)
                               ggCTATATGACAGATATGCAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAg                                                                                                                 <  1:55139/143‑1 (MQ=255)
                                gCTATATCACAGATATGCAACGTATTGCTGTGGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                                                                                                <  1:777059/143‑1 (MQ=255)
                                 cTATATGACAGATATGAGACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCTCGCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCa                                                                                                               <  2:233723/143‑1 (MQ=255)
                                  tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAc                                                                                                              >  1:480391/1‑143 (MQ=255)
                                  tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCCTCCCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGccc                                                                                                              >  2:508546/1‑141 (MQ=255)
                                   atatGACAGATATGAAACGTATTGCTGTTGCGGGATAAGTGCGCGCTGATGCCCTCACCCCGACGCTCTCCCCCAGGGAGAGGGCGAAAACACTCAAGGCCTTCTGCTGGAGAAGGGCTCGCTCGTAGTTGCGATAGAGctga                                                                                                             >  2:717159/1‑140 (MQ=37)
                                        aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                                                                        <  1:666710/143‑1 (MQ=255)
                                         tagagATGAAACGTATTGCTGTTGAGGGTTAAGTGCGTGCTGATGACCTCACCCCGACAATCCGGTAAAGCTTTAGGGAGAAAACACTCAAGGCCATCTCATCGCGAAGCCCTTATCAATAGTTGCGGTACAGCACCTTAatg                                                                                                       <  2:685559/142‑1 (MQ=255)
                                            ctctGAAGCGTCTTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                                                    <  1:67814/140‑1 (MQ=255)
                                             tgtgacgcGTATTGCTGTCGCGGGGTGAGTGGGCGTTGCTGCTCTCAGCCCGGCCCTCTCCCACAGGGAGAGGGAGGAAACACTCAAGGGCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                                   <  2:151529/136‑1 (MQ=37)
                                             tatGAAGCGTATGGCTGCTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCGGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                                   <  2:601382/143‑1 (MQ=255)
                                             tatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCCCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                                   <  1:72188/143‑1 (MQ=255)
                                              atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                                                                                  <  1:583620/143‑1 (MQ=255)
                                                 aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata                                                                                               >  1:117376/1‑143 (MQ=255)
                                                 aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACCCAAGGCCTTCTCCTGGCGCAGCCCCCGCTCTCAGCTGCGGTACAGCGCCTTGATGATGCGaga                                                                                               >  2:52222/1‑141 (MQ=25)
                                                 aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACACCGACCCTCTCCCACAGGGAGAGGGAGAAAACGCTCAAGGCCTTCTCCTGGAGCAGGCCTTGCTATTAGTTGAGGTACAGGAACTGAATGCTGTAata                                                                                               >  1:773637/1‑143 (MQ=37)
                                                  gacgttgtGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACTCCTACAGGGAGAGGGAGACAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGGTGCGGTACAGCACCTTAATGATGTGatat                                                                                              <  2:681035/136‑1 (MQ=255)
                                                  aaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGatat                                                                                              >  1:818616/1‑143 (MQ=255)
                                                   acctATTCCAGTTGCGTGTTAAGTTCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                                             <  1:522353/140‑1 (MQ=38)
                                                    cGTATTGCTTTTGTGGGTTAATTGCGCGCTGAGGAGCACACCCCGACCCGGTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc                                                                                            <  1:321911/143‑1 (MQ=255)
                                                           cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGGAGCAGAAAACACTCAAGGCCTTCTCCTGCAGAAGCCCTCGCTATTAGTTGCGGTACAGCACCTTAATGACGCGACAGCCGaccgcg                                                                                     >  2:765940/1‑138 (MQ=37)
                                                           cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGCAAACACTCAAGGCCTTCTCCTGGAGAAGCCCTTGCTATTAGTTGCGGTACAGCACACTACTGATGTGACATCCGAACTgg                                                                                     >  1:385677/1‑143 (MQ=38)
                                                           cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                                     <  1:564897/143‑1 (MQ=255)
                                                             ggtGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                                                   <  1:337363/141‑1 (MQ=255)
                                                              ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCGCAGGGAGAGGGAGCAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGGACAGCACCTTACTGATGTGCTACCCGAACAggggg                                                                                  >  2:724905/1‑141 (MQ=37)
                                                               tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtgt                                                                                 <  1:21928/143‑1 (MQ=255)
                                                                     ttAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTGATGAGGTGATATCCGAACTGGGTGTGCAGCg                                                                           >  1:755472/1‑143 (MQ=255)
                                                                     tgaaGTGCGGGCTGATGCCCTGCCGCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                                                                           <  2:348779/141‑1 (MQ=255)
                                                                       aaGTGCGGGCTGATGCCCTCACCCCGACCCGCTCCCACAGGGAGAGGGAGAAAACACGCAGGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGCTGCGCTCTCCGAACTGGGTGTGCAGCggg                                                                         <  2:513622/143‑1 (MQ=37)
                                                                       aaGTGCGCGCGGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCggg                                                                         <  1:745567/143‑1 (MQ=255)
                                                                        ggtccgcgcAGATGCCCTCACCCCGACCCTCGCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                                                                        <  1:791908/139‑1 (MQ=255)
                                                                              cgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGCGAGGGAGCAAACACTCAAGGCCTTCTCCTGGAGACGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCCGGCCGGTCg                                                                  >  2:466015/1‑143 (MQ=255)
                                                                              cgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTCCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTCAATGCTGTGATATCCGAACTGGGTGTGCAGCGGgccggccg                                                                  >  2:68447/1‑143 (MQ=255)
                                                                               ggtGACGCCCGCCCCCCGACCCTCTCCCCCAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCGTGCTATTAGTTGCGGTGCAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                                                 <  2:576586/141‑1 (MQ=255)
                                                                               gcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                                                 >  1:279027/1‑143 (MQ=255)
                                                                                    tGCCCTCACCCCGACCCTCGCCCCCAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAGGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                            <  2:766604/143‑1 (MQ=255)
                                                                                    tGCCCTCACCCCGACCCGCTCCCACAGGGAGAGGGAGAAACCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                            <  2:249022/143‑1 (MQ=255)
                                                                                     gCCCTCCCCCCGCCCCTCCCCCACGGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGTAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAg                                                           <  2:360484/143‑1 (MQ=255)
                                                                                         tCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAAAACTCAAGGACTTCTCATGGAGCAGGGTTTGCTATTAGTTGCGGTACGGAGCCTTAGTGATGTGATATCCGCACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                       >  1:559378/1‑143 (MQ=255)
                                                                                          cgccgcGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg                                                      <  2:167023/138‑1 (MQ=255)
                                                                                          cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg                                                      >  1:420783/1‑143 (MQ=255)
                                                                                                   ccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCGGGTCGGCTCCAGTACCGGACAAgaga                                             >  1:706344/1‑143 (MQ=255)
                                                                                                   ccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAgaga                                             >  1:205185/1‑143 (MQ=255)
                                                                                                   ccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAGTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGACGGAGTCAGGACCGGAACagagg                                             >  2:652919/1‑142 (MQ=255)
                                                                                                    ctctCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                                            >  2:436648/1‑143 (MQ=255)
                                                                                                     tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGgaa                                           >  2:521800/1‑143 (MQ=255)
                                                                                                      ctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGGAGGGGGCCGGCCGGCTCCAGTACCGGACAAGAGgaaa                                          >  1:524970/1‑143 (MQ=255)
                                                                                                      ctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGCCAAGaaaaaa                                          >  2:324395/1‑143 (MQ=255)
                                                                                                       tcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCGGCACCGGACACGAGAAAAc                                         >  2:397540/1‑143 (MQ=255)
                                                                                                            caGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAcct                                    >  2:532733/1‑141 (MQ=255)
                                                                                                            caGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt                                    >  1:486396/1‑143 (MQ=255)
                                                                                                                     ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACg                           >  1:300177/1‑143 (MQ=255)
                                                                                                                     ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATAGAACg                           >  1:734367/1‑143 (MQ=255)
                                                                                                                        gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCg                        <  2:42476/143‑1 (MQ=255)
                                                                                                                         aaaaCACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCgg                       <  1:740353/143‑1 (MQ=255)
                                                                                                                          aaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTGGTCGCAATAAAGACCCTTCATTAGGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGa                      <  1:191660/143‑1 (MQ=255)
                                                                                                                           taatagcAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGaa                     >  1:684238/7‑143 (MQ=255)
                                                                                                                               cTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGCTATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTACCGAACGCCGGAACCAt                 >  1:412581/1‑143 (MQ=255)
                                                                                                                                tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGCGAAAACGACCTTATCGAACGCCGGCACCATc                >  2:289337/1‑143 (MQ=255)
                                                                                                                                tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATc                >  1:553453/1‑143 (MQ=255)
                                                                                                                                tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAACGGACAAAAGAAAACCACTTTATCGAACGCCGGAAGCATc                >  2:679836/1‑143 (MQ=255)
                                                                                                                                 cacggCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCAGATCCAGTACCGTACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCt               <  1:356284/140‑1 (MQ=255)
                                                                                                                                 cAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCt               >  1:640582/1‑143 (MQ=255)
                                                                                                                                  aaGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGCTATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACTCCGGAACCATCTg              >  1:112148/1‑143 (MQ=255)
                                                                                                                                  aaGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGACCCATCTg              >  1:303560/1‑143 (MQ=255)
                                                                                                                                  aaGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTg              >  1:794494/1‑143 (MQ=255)
                                                                                                                                    ggCCTTCTCCAGAAGAAGACTTTGCGATTAATTGCGGGACAGCACCTTAATGATGTGATATCCCAGCTGGGGGTGCAGCGGGCCGGTCAGCTCCCGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAtacggc            >  2:465747/1‑138 (MQ=255)
                                                                                                                                          ctcCTGGAGAAGGCCTTGCTATTAGTTGCGGTCCAGCACCTTAAGGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGc      <  1:154101/143‑1 (MQ=255)
                                                                                                                                           tcCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGCTAACCGAACTGGGTGTGCAGCGCGCCGCTCGGCCCCACTACCGGACAAGCGAAAACCACATCATCGAACGCCGGGACCCTCTGACCCTgccc     >  2:351201/1‑140 (MQ=255)
                                                                                                                                           tcCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGCGCCGGTCGGCGCGGCGACGTTACTAGAGAAAACCAATTTATCGAACGCCGGAACCATCTGACCCTGGCg     >  1:387266/1‑143 (MQ=255)
                                                                                                                                            ccTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCACCTGACCCTGGCgg    >  2:545137/1‑143 (MQ=255)
                                                                                                                                             cTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGACCGCCGGAACCATCTGACCCTGGCggt   >  1:217766/1‑142 (MQ=255)
                                                                                                                                              tGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa  >  1:407322/1‑143 (MQ=255)
                                                                                                                                              tGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAGGTGATATCCGCACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGCACCGGACAAGAGGAAACCACTTTGTCGTACGCCGGGACCATCTGACCCTGGCGGag  >  1:155250/1‑142 (MQ=255)
                                                                                                                                              |                                                                                                                                              
TGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAA  >  NC_000913/3959373‑3959657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑