Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,416,239	C→T	T498M (ACG→ATG)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,416,239	0	C	T	93.5%	79.4 / NA	31	T498M (ACG→ATG)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (14/15);  minor base G (2/0);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGGTGCAGGGATTGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTT  >  NC_000913/2416099‑2416371                                                                                                                                             |                                                                                                                                     gcggggTGCAGGTAGTGTAGTCGGTGATCTGGAAGTGGTTGTCGAAGGCTATGTTGGTCGTCGGGTGGTACTGCGTAACAACAAGTGCATGTTCGAGACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGc                                                                                                                                    <  2:103900/140‑1 (MQ=255)     ggTGCAGGGATTGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGAt                                                                                                                                <  1:517098/143‑1 (MQ=255)        gCAGGGATTGAAATCGTTGATCCAGAGGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACACAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGACGACAACGTGGTGCTCCGTATGCTGAGGCt                                                                                                                             >  2:344313/1‑143 (MQ=255)         cAGGGATTGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGGTGCCCGCGAACAGCTGGAAGACAACGTGGAGCACGGTAGGCGGATGccg                                                                                                                            >  2:331236/1‑141 (MQ=255)          aGGGATTGAAATCGTTGTTCCAGAAGTGGTTCGGGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTgg                                                                                                                           <  2:208464/143‑1 (MQ=255)              aTTGGAATCGTTGATCCAGAAGCGGTTCGCGAAAGCTATGCTGGTCCTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAGCCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACa                                                                                                                       <  2:312722/143‑1 (MQ=255)              aTTGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGGAAGAACAAAGGCATGACCGAAACCGTAGCCCGCGAACAGCTGAAAGAAAACGTGGTGCGCGGCATGCGGACACTGGAACa                                                                                                                       >  2:432315/1‑143 (MQ=255)                     tCGGTGACCCAGAAGGGGTTCGCGAAAGCTATGTTGGTCGTCCGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGaa                                                                                                                <  1:302294/143‑1 (MQ=255)                       gTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGACGACAACGTGGTCCTCGGTCTGCTGATGTCGGAACAGCGTGAAGt                                                                                                              >  1:562872/1‑143 (MQ=255)                          gATCCAGAAGTGGGTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGa                                                                                                           <  2:284787/143‑1 (MQ=255)                            tCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAGCAGCTGGAAGACAACGTGGTGCTCGGTATGCTTATGCGGGACCAGGGCGAAGCTgctc                                                                                                         >  1:225691/1‑140 (MQ=255)                              cAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGCAGTTGATGGt                                                                                                       >  1:440972/1‑143 (MQ=255)                                gAAGTGGTGCGCTAAAGCTATGTTGGTCGCCTGGTCGAACTGCGTAAGAACAGAGGCACGACCGGAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCt                                                                                                     <  2:583793/143‑1 (MQ=255)                                 aaGTGGTTCGCGACAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTg                                                                                                    <  1:435900/143‑1 (MQ=255)                                        tCGCGCAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGATCAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCg                                                                                             <  1:157180/143‑1 (MQ=255)                                        tCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACCGGATGAAGTTGATGGTCTGGTTTCCg                                                                                             >  1:273681/1‑143 (MQ=255)                                           cgAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAACTCGAAGACAACGCGGGGCTCCGTATGCTGAGGCCGGAACAGGACGAAGCTGATGGTCTGGCCTCCGGTg                                                                                          >  2:273818/1‑143 (MQ=255)                                           cgAAAGCTATGTTGGTCGTCTGGTCGAACTGCCTAAGAACCAAGGCATGACCGAAACCGTTGCCCCCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCGGAAGCCGGAACAGGATGAAGCCGCTGGCCTGGTCTCCGGtc                                                                                          >  2:603385/1‑142 (MQ=255)                                               aGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGAAGCTGAATAAAGAAGAAGGTGATGGTCTGGTTTCCGGTGCTGt                                                                                      >  1:591262/1‑143 (MQ=255)                                                        ggTCGTGTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTAc                                                                             <  1:445100/143‑1 (MQ=255)                                                                             aaGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTGGGTCGGCTGATGCTGGCACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGGTCACACTACCGCAAACACCATCCCTccgcc                                                        >  2:356979/1‑143 (MQ=255)                                                                             aaGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTccgcc                                                        >  2:1873/1‑143 (MQ=255)                                                                                               gAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAAc                                      <  1:542816/143‑1 (MQ=255)                                                                                                   ccGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCa                                  >  2:428910/1‑143 (MQ=255)                                                                                                    cGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCCAAGCTGCAc                                 >  1:580947/1‑143 (MQ=255)                                                                                                           cgcgAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTAATCAGACCTGCCCCGGGCAg                          >  2:572995/1‑143 (MQ=255)                                                                                                                  aGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTg                   <  1:114225/143‑1 (MQ=255)                                                                                                                          gACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTc           >  2:17045/1‑143 (MQ=255)                                                                                                                                 tggtgCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGttc    <  1:544967/143‑1 (MQ=255)                                                                                                                                 tggtgCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGttc    <  2:564966/143‑1 (MQ=255)                                                                                                                                   gtgCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGttctt  <  2:326893/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                     ACTGGGTGCAGGGATTGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTT  >  NC_000913/2416099‑2416371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑