Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,026,927	A→T	V30E (GTG→GAG)	mgsA ←	methylglyoxal synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,026,927	0	A	T	96.3%	74.1 / NA	28	V30E (GTG→GAG)	mgsA	methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (11/15);  minor base G (0/1);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTA  >  NC_000913/1026787‑1027062                                                                                                                                             |                                                                                                                                        aTGCGCCAACCTCCTGGGCACCCCCCATTGCGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTtcctcc                                                                                                                                       <  1:448015/143‑1 (MQ=255)       gagccTGCGGGCCACCCCCCTTTGGGGCACTCTGCATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTACCGCTTGATGCCGTTCCTCCCAGCtc                                                                                                                                 <  2:9223/140‑1 (MQ=255)             gctgGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCCGc                                                                                                                           >  1:527515/1‑143 (MQ=255)                   cACCCCCCAGTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTTTGCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCAtttgt                                                                                                                     <  1:76986/143‑1 (MQ=255)                      ccccccTTGGGCCCCTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCAtttgtttg                                                                                                                  <  2:400713/143‑1 (MQ=255)                       ccccATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGGCGTTCCTCCCCGCTCATCAGCATTTGTTTGc                                                                                                                 >  1:70277/1‑143 (MQ=255)                        cccATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCa                                                                                                                <  2:292268/143‑1 (MQ=255)                              gggCCGCTCAACATCGCGTGGACGTTCAGGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATc                                                                                                          <  1:127779/143‑1 (MQ=255)                                  cACTCACCATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCgtgt                                                                                                      <  1:480372/143‑1 (MQ=255)                                   aCTCACCAGCGCGTTGACCTGCATGCCGGTCGCGCGGGAAAGTAATTTACCGGTAGTGCCTGTTGCATACTGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGtgtg                                                                                                     <  2:6851/143‑1 (MQ=255)                                         cATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGccacca                                                                                               >  2:474564/1‑143 (MQ=255)                                           tCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAgc                                                                                             >  2:527576/1‑143 (MQ=255)                                                      ttCATGCCGGGCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGttt                                                                                  <  1:415758/143‑1 (MQ=255)                                                          tGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCcgc                                                                              >  2:152647/1‑143 (MQ=255)                                                             cGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCa                                                                           <  1:452710/143‑1 (MQ=255)                                                                         aaaTTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTACCGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGa                                                               <  2:214525/143‑1 (MQ=255)                                                                                   aCCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGtt                                                     <  1:373128/143‑1 (MQ=255)                                                                                     cGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCCCGCAGGTAACGTGCGAGTCGTCAGTTcc                                                   >  2:586363/1‑143 (MQ=255)                                                                                           tGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATg                                             <  1:480103/143‑1 (MQ=255)                                                                                           tGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATATGTTTGCAGTGATCGTGTGCCACCAGAGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATaacg                                             >  2:208717/1‑141 (MQ=255)                                                                                            gCCGGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGt                                            <  1:610646/143‑1 (MQ=255)                                                                                              cTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCGGGTAAAGTGCGAGTCGTCAGTTCCATAATGtcc                                          >  1:266357/1‑141 (MQ=255)                                                                                                   gCATACAGTCCGTGTTGTCCCAGGAACGGTTGATGCCGTCCCTCCCAGCTCATCAGCATGTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCg                                     <  1:576808/143‑1 (MQ=255)                                                                                                      tACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAg                                  >  1:223745/1‑143 (MQ=255)                                                                                                                     tCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGGCACTTCCATAATGTACACCCGTAGTTAACTTCCCTCCaa                   >  2:81740/1‑142 (MQ=255)                                                                                                                      ccAGCAACGGTTGCTGCCGTTCCGCCCAGCCCTTCAGCCTTTGTTTGCAGTGACCGCGTGCCACCGCCGCCATATTTTGCCGCGCAGGTAAAGTGCGAGTCGGCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGa                  <  1:203022/143‑1 (MQ=255)                                                                                                                               gTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTaa         >  1:389860/1‑143 (MQ=255)                                                                                                                                 tGATGCCGTTCCTCCCAGCTCATCAGCATGTGTTTGCAGTGATCGTGTTCCACCAGCGCAATATGTTTCCGCTCATGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGc       <  2:73564/143‑1 (MQ=255)                                                                                                                                      ccGTCCCCCCAAGCCCACCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTCACTTTCCTACAGATTACTGTAAGCACTTa  <  1:440854/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                        ATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTA  >  NC_000913/1026787‑1027062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑