Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	4,001,645	Δ5 bp	100%	coding (220‑224/951 nt)	corA →	Ni(2(+))/Co(2(+))/Mg(2(+)) transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	1 (0.040)	52 (2.130)	45/264	NT	98.1%	coding (219/951 nt)	corA	Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
	?	NC_000913	4001650 =	1 (0.040)					coding (225/951 nt)	corA	Ni(2(+))/Co(2(+))/Mg(2(+)) transporter

TATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001505‑4001644 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGC  >  NC_000913/4001650‑4001788                                                                                                                                                                                                                                                                                         TATGGATTGATCTTGTCGAACCGGACGAGGACGAGCTACTGCGGTTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCTTCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTC                                                                                                                                          <  2:489445/143‑1     gcTTGATTTTGTCGTGCCGTACGGCGACGAGCGGCTTCGCGTACAAGCTGAACTTGGCCAGAGCCTGGCTTCCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTC                                                                                                                                      <  2:338281/141‑1     GATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACCATCTGAACTTGGCCAGAGCCTGGCAACCCGAACGGCACTGGAAGACAACGAAGCATCGGAACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCCTC                                                                                                                                      >  1:406610/1‑143          ATCTTGTCGAACCGGCCGACGACGAGCGACTGCGCGTACGCTCTGAACTTGGTCAGTGCATGGTAACTAGTTTGGAAGTGGAAGACATAGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTT                                                                                                                                 >  1:181847/1‑143           TCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCAGCACGATTCTTTAAAGACGACGACGGCATGCATCTCCTTATTCTTC                                                                                                                                >  1:238391/1‑143              TGTCGAACCGGACGACGACGAGCGACTGTGCGTACAATCTGACCTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTT                                                                                                                             <  1:422011/143‑1               GTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGATCTTGGCTTGAGTCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATTGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTG                                                                                                                            <  1:294877/143‑1               GTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGAAGACGGCCTGCATCTCCTTCTTCTTCTTTG                                                                                                                            >  1:418293/1‑143                TCGATCCTGACGACGACGAGCGACTGCGCGTACAATCTGGACTTGGCCAGAGCCTTGCAACCCGCCCGGAACTGGAAGACATCGTAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGA                                                                                                                           <  2:331805/143‑1                TCGATCCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGA                                                                                                                           <  2:378824/143‑1                TCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTAGAAGACATCGAAGCATAGGAACGTATCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGg                                                                                                                           >  1:15419/1‑142                 CGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGATCTTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAA                                                                                                                          <  1:173651/143‑1                 CGAACCGCACTACGCCGAGCGACTTCGCGTACAATCTGAACTTGGCCAGAGCCTGGCTACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAA                                                                                                                          <  2:183154/143‑1                     CCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATG                                                                                                                      <  2:319365/143‑1                      ccGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAACACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGC                                                                                                                     <  2:157128/141‑1                          CGACGACGAGCGACTGCGCGTACAATCTGATCTTGGTCAGAGTTTTGGAATTCGCCCGGAACTGGAAGAGATCGAAGGAGCGGCACGTGTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAA                                                                                                                 <  1:414798/143‑1                          CGACCACGCGCGATTGCGCGTACAATCTGAACCTGGCCAGAGCCTGGCAACCCGCCCGGAACCGGCAGCCATCGATGCATCGCCACGTTTCTTTGAAGACGACGACGGCCTGCATCTGCTTCTTCTTCTTTGAAGATGCGGAA                                                                                                                 <  2:480142/143‑1                            ACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGA                                                                                                               <  2:389591/143‑1                             CGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGAT                                                                                                              <  1:88535/143‑1                               ACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACTCGTCCGTAACTGGAAGATATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCA                                                                                                            <  1:25764/143‑1                                 GAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCCACCCGCCCGGAACTGGAAGACATCGAAGCATCAGCACGTAACATTAAAAACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCAGAAGATCAag                                                                                                          >  1:394618/1‑141                                   GCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGAATCGGCACGTTTCTTTGAAAACGACAACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCC                                                                                                        >  1:402687/1‑143                                        TGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTGTCGGTGCAGACGACGACGGCCTGCAGCGCCTTCTGCTGCTTGGCAGATGCGGAAGACAACGCGGGAAA                                                                                                   >  2:360221/1‑143                                           GCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGAAAATTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTC                                                                                                >  1:405382/1‑143                                              TACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGG                                                                                                      <  2:80905/134‑1                                              TACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGG                                                                                                      >  1:80905/1‑134                                              TACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGCCACGTTTCTTTGAAGACGACGACGACCTACAACTCCTTCTTCTACTTTGAAGATGCGAAAGATCACGCCGGTAACTCCAC                                                                                             >  1:257916/1‑143                                                 tATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGTACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGT                                                                                          <  1:301442/142‑1                                                 AATCTGAACTTGGTCAGATCCTGGCTATCTGCTCGTAACTGGAAGACATCGAAGCATCGGCACGTGTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGT                                                                                          <  1:174220/143‑1                                                  cTCTGAACTTTGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTG                                                                                         <  2:404324/142‑1                                                     TGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATAACGCCGGTAAATCCACTGTGGCA                                                                                      >  1:251458/1‑143                                                         CTTGGCCAGAGCCTGGCAACCCTCTCGGATCTGGTTGTTTTCAAAACATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCGCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTA                                                                                  <  1:141569/143‑1                                                          tttGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTGCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAC                                                                                 <  1:55343/140‑1                                                          TTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGACGCCGCCTCCGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCAGAAGATCAAGCAGGTAACTCCACTATGGCATTTAC                                                                                 >  1:468868/1‑143                                                          TTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATTCAGAAATTCCTCTGGAAAGATCCGGAAGAACACGCCGGTAACTCCACTGTGGCATTTAC                                                                                 >  1:489235/1‑143                                                               CAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTAAAGATGCGGAAGATAACGCCGGTAACTCCACTGTGGCATTTAACATat                                                                            >  1:430229/1‑141                                                                          AACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTACATCTCCTTTTTCTTATTAAAAAATGAGGAAGATAACGCAGGTAACTACACTGTGGGATCTACCATCCGTGCTGGTCGT                                                                 >  1:490668/1‑143                                                                                  CGGAGCTGGAAGTCGTCGAAGCATGGGCACGTTTCTTTGAAGACGACGACGGCCTGCAGCTCCTTCTTCGTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAC                                                         <  1:339761/143‑1                                                                                        TGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGAAAAAACCGGTAACTCCACTATGGCATTTACCATCCATGATGATAGTCTCTTTACTCTGCc                                                   >  1:381127/1‑142                                                                                         GGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCTTTCTTCTTCTTTTAATATACATATGATCACGCCGGTAACGCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGT                                                  <  1:267810/143‑1                                                                                            AGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCAAGCCGGTAACTCCACTGTGGCATTTACAATCCGTGATGGTCGTCTGTTTACTCTGCGTGAG                                               >  1:431648/1‑143                                                                                             GACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACTGTCTGTTTTTTTTTTTTTTTCTTTGAAGATGCGGAAGATCACGGCGGTAACACCACGGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGC                                              <  1:41574/143‑1                                                                                               CATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCCGCATCTCCTTCTTCTTCTTTGATGTTGCGGAAGATCACGCCGGTAACGCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGT                                            <  1:25873/143‑1                                                                                                 TCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCCGAAGTTCACGCCGGTAACTCCACTGTGGCATTTACCATCCGGGAAGGGCGGCTGTTGACTCTGCATGAGCGTtg                                          >  2:27938/1‑141                                                                                                  ccaatCATCGGCGCGTATCTCTGAAGACGACGACGGCCTGCAGCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                                         <  1:277973/138‑1                                                                                                  CGAAGCATCGGCACGTTTCTTTGAATATGACGACGGCCTGCATCTCCTACTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                                         <  1:208240/143‑1                                                                                                            GCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTT                               >  1:430330/1‑143                                                                                                               CGTTTCTTTGAAGGCGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTC                            <  2:452565/143‑1                                                                                                                  TTCTTTCAAGACGACGTGGGTCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGTGAACTCCACTGTGTCATTTGCCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTC                         <  2:404847/143‑1                                                                                                                    CTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGCTCGACTGTTTACTCTGCATGAGCGTGAACTGCCCGCTTTTCGTCTG                       >  1:307554/1‑143                                                                                                                     TTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCAATAAAAATACATGATGGACGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTCTTCGTCTGT                      >  1:182594/1‑143                                                                                                                          AGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCAGTGATAGTCGTCTAATTACTCTGCGTGAGCGTGAACTGCCCACTTTTCGTCTGTATCGT                 >  1:413338/1‑143                                                                                                                                CGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGCTCACGCCGGTAACTCCACTGTGACATTTACAATAAGAGATGGTCGTCTGTTTACTATGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGT           >  1:285/1‑143                                                                                                                                         GCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATTGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGC  <  2:55418/143‑1                                                                                                                                                                                                                                                                                         TATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001505‑4001644 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGC  >  NC_000913/4001650‑4001788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.