| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,940,584 | C→A | 100% | R351L (CGT→CTT) | mepM ← | peptidoglycan DD‑endopeptidase MepM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,940,584 | 0 | C | A | 100.0% | 44.4 / NA | 19 | R351L (CGT→CTT) | mepM | peptidoglycan DD‑endopeptidase MepM |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (9/10); total (9/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGT > NC_000913/1940451‑1940714 | gTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGCATCTTGCGCAAGTGCATATAACGCGCGGTGTAGCTGCGACCATGCAGAATCGCca > 2:350905/1‑143 (MQ=255) aGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATCCGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAAAATAATAAGCAAGTGCATATAACGCGTGGTGTAGCTGCGACAATGAAGAATAGCCACata > 1:86250/1‑143 (MQ=255) ctggggCCCGGTTGAACGTCGGGTATTACCGGGAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACataat < 2:446124/139‑1 (MQ=255) tGCGGCCCGGTTGAACGTCCGGTATTAACGGAAAGCGCGATACGGTCACCACGTTTCACCATCTGTCCCGGTTTGACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGATGCGACAATGAAGAATAGGCACataata > 2:462469/1‑143 (MQ=255) cGGCCCGGGTGAACGTCCGTTATTACCGGAAAGCGCGATACTGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATTACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAAc < 2:144292/143‑1 (MQ=255) cGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCTCCTCGTTTCACCTTCTGTGCCGGTTGCACCAGAAACTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACctgct < 1:295292/143‑1 (MQ=255) ggTTGAGCGTCCGGGAGTGGCGGACAGCGCGTGACGGTCGCCTCGTTTCCGCGTGTGTCGCGGTTTCACCAGAATCTTGCGCACGTTCAGATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACctgctg < 2:109864/143‑1 (MQ=255) aCCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCaa > 1:363808/1‑143 (MQ=255) cgcgTTACGATCGCAACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCAGATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACtt < 2:473278/143‑1 (MQ=255) gATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCAc > 2:458087/1‑143 (MQ=255) gCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTTCATCTAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCAACCa > 1:161846/1‑143 (MQ=255) taCGTTTCTCCTTCGGTTCGGGTTTCACCAGAATCTTGCGCATGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACt < 2:72998/142‑1 (MQ=255) ccGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAACCACATAATAACCTGCTGCGCCACTGCGTTTGACCACCACCACTACACAATCACACACTGAAAGCACTGGCGTAccc > 2:283546/1‑143 (MQ=255) cGGTTTCACCAGAATCTTGAACAAGAGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCACCCACCACCTCACCGTCACCCACTGACACCACTGGCGTCCCCt > 2:53571/1‑143 (MQ=255) ggTTTCACCAGAATGTTGCGCAATTTCATATAACGCGTGGTGTAGCTGCGACTATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTg < 1:25465/143‑1 (MQ=255) ttCACCAGAATCTCGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCgg < 1:45076/143‑1 (MQ=255) ataACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCtgtgtg < 1:458087/143‑1 (MQ=255) aGCTGCGACCATGAAGAATAGCCACCTCATAACCTGCTGCGCCACTGCGTGTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCg < 2:398518/143‑1 (MQ=255) cTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGCCACCCACTGAAACAAACGCCCTACCCCGCGCCATGGCGAAATCACCACCTCAGCACGATGCACCGCGACCgat > 2:155957/1‑141 (MQ=255) | GTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGT > NC_000913/1940451‑1940714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |