| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,941,326 | C→T | 100% | D104N (GAT→AAT) | mepM ← | peptidoglycan DD‑endopeptidase MepM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,941,326 | 0 | C | T | 100.0% | 46.8 / NA | 20 | D104N (GAT→AAT) | mepM | peptidoglycan DD‑endopeptidase MepM |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/10); total (10/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAG > NC_000913/1941187‑1941452 | tggaGTTCGCCGTCCTCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGcc < 2:147739/140‑1 (MQ=255)tGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTATTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGAGCTATCACCCATATCAATACCATACTGATTAAGAATGCTACTTAGCGGATTGcc > 2:177086/1‑143 (MQ=255) ttCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAACGTATTGCCAGTgg > 2:40559/1‑143 (MQ=255) cGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCACACTGATTAAGAATGCTGCTTAGAGTATTGACAGTCGaa > 2:272143/1‑143 (MQ=255) gCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGTCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGGTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGaaa < 1:308395/143‑1 (MQ=255) aCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTCAGCGTATTGCCAGCGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGa > 2:163072/1‑143 (MQ=255) aaTTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTc < 2:12628/143‑1 (MQ=255) gTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGCTTGAGACTGCTGCTTAGCATATTGCCAAAGGAAACAACATATTAATACACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGaa > 1:254406/1‑143 (MQ=255) taGTTGGGTGATATCTCCCATATCAATACCTTGTTGTTTGAGAATGTTTCTTAGTGTTTTTTCAGTGGAAACAACAGATTCATGCACGCCCGCTTCACCGGCGATTTGGTCATCCAGTTCGTCCTGGGGAATGGCTTCAtctt < 1:329797/142‑1 (MQ=255) ggTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATCTTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTtgtg > 1:317777/1‑143 (MQ=255) aCCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCCGTGGAAACAACCTATTCCTGCACGCCCGCTTCACCGGCGATTCTGTCATCCATTTAGTCCTACGGAATGACATCATCTTCTTGTCCCGCTTGAt > 1:167328/1‑143 (MQ=255) tgatgctaaTGCCATACTGATTGCGAATGCTGTTTAGCTTTTTGCCTGTGCTAACCACATATTCATTCACGCCCGCTTCACCGGCGATTTTGTTATACAGTTCGTTCTTGTTAATTGCTTCATCTTCTTGTGCAGCTTGATCa < 2:333109/136‑1 (MQ=255) atatCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGATACTACATATTCATACAAGACAGCAACACAGGAGAATTAGTCATCCAGTTCGTCATGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAAt > 1:6375/1‑143 (MQ=255) tatCAATACCATACTGATGGAGAGTGCTGCTTAGCGGAGTGCCAGTTGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGTTTTATCTTCTTGTGCAGCTTGATCAATc < 2:126467/143‑1 (MQ=255) tCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCTCGCCCGTTTTACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCgg < 1:275873/143‑1 (MQ=255) aTACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTgg > 2:269640/1‑143 (MQ=255) cTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTTTTTTACGTCTGTTTCTCCTGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCtt < 1:5633/143‑1 (MQ=255) gATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTGTTGTGCAGCATGATCAATCGGCTCACTGGCttga > 2:299429/1‑141 (MQ=255) agaATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGTCCTCTTCTTCGGTGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTaa < 1:40559/143‑1 (MQ=255) aTGCTGCTTAGCGTATTGCCAGTGGACACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAagag < 2:103935/143‑1 (MQ=255) | TGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGCTTCAGGTAAGAG > NC_000913/1941187‑1941452 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |