| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,956,975 | G→T | 100% | H11Q (CAC→CAA) | torZ ← | trimethylamine N‑oxide reductase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,956,975 | 0 | G | T | 96.9% | 72.9 / ‑5.8 | 26 | H11Q (CAC→CAA) | torZ | trimethylamine N‑oxide reductase 2 |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (13/12); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTGTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTCCAGTTCGTTTTCGC > NC_000913/1956833‑1957102 | aagaaACAATCTTGCCGTCCTTCACTTATACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGGTGCCGATGTCAGCACCAGCGGTCCAGCGGCAATACCGCtg > 2:431282/1‑143 (MQ=255) cAATCTTGCCGTCCTTCTCTTCTTCCTTCCTCTTTCCCTATTGTTCTGTGGTGTGGATTTTACGGCCCAAAGCGGCGGCCCAGGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTta < 1:114443/143‑1 (MQ=255) atttttaTCGCTTCCCATCGTTCCTCTGTTAGGATTTTACCGCCTTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGAGGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGt < 1:195024/137‑1 (MQ=255) gCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGAGGAGCGGTGATGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTtc > 1:224852/1‑143 (MQ=255) ccacggTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTTTCCATGCGGTTTTTTGTGCTGTTGATGTCACCACCAGCGCTGCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTccc < 1:249072/138‑1 (MQ=255) ccATCGTCCCGCTGTGATTATCTTACCTCCCTTCTTTTCTTCTTTTTTGTTTTGCGGTGTTGCCGATGTCACCACCAGCGCGCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTccc < 1:249078/143‑1 (MQ=255) ccAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCCCCAGCGCTCCAGCGGCAATACCGCTTTATTTAAAAAAATAACGTCTTGTTAATGTCATAACTTCCTccc > 1:472401/1‑143 (MQ=255) aaCGTCCCGCGTTGAGTATTTTACCGTGTTTCGCTTCTTCCCATGCGGTGAGCTGTACTGCCGATTTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTg < 1:321992/143‑1 (MQ=255) gcgccccGCGAGGAGGATTGTACCGCCCTTCTCTTCTGACCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGa < 2:321579/139‑1 (MQ=255) aCGTCCTGCGGTGAGGATTTTTCCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGa < 2:424217/143‑1 (MQ=255) ccTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGATCAACGAGGACGAGTGTTGCTCGGTa > 2:367494/1‑143 (MQ=255) tcttctGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAatata > 1:136492/1‑142 (MQ=255) ccATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTGTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGATCAACGCGGATCACTGTTTCTCGGTTCTATCTGTGGCGt > 2:288873/1‑143 (MQ=255) ggAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATCAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGATCATACAGAATCACAATTTCTCGGTAATATCTTTGGCGTTGTACTgg > 1:323064/1‑143 (MQ=255) gtggggTGTTGTCGTTGTCTCCCCTCGCGCTCTAGCGGGGAGCCGGCTTTCTTTTATATATTCCCGTCTTGCTACGGTCATACCTTTTTCTTTGATAAACGAGGATCTTTCTTTCTCGGTAATATCTTTGGCGTTGTACTGGa < 2:242622/139‑1 (MQ=255) ctgcCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCTCGTCTTGTTAATGTCATTATTTCTTTCTTGATCAACGAGGATCACGGTTGCTCGGTAATAGCTTTGGCGTTGTACTGGAAATACcgc < 1:311549/143‑1 (MQ=255) ccGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGACATAACATCATACCTGATCAACGAGGATCACAGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGtt > 1:382643/1‑143 (MQ=255) cGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTa > 2:239254/1‑143 (MQ=255) tCACCACCAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTACTGTCTTCACTTCCTCCCTGATCAACGAGAATCACTATTTCTCGGTAATATCTTTGACGTTGTACTGGAAATACCGCGTTAAAATg > 1:47489/1‑143 (MQ=255) accaccAGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTc < 1:239254/143‑1 (MQ=255) caccaGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTTCTCCCTGATCATCGAGTATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTCCa < 1:368194/143‑1 (MQ=255) caGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCTTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTCCAGtt > 1:14121/1‑143 (MQ=255) caGCGCTCCAGCGGCAAAACCGCAGTGTTTAATAAATTCACGTCTTGTTAATGTCATAACGTACTCCCTGATCAACGAGGATCACTGTTTCTCGGTAGTATCTTTGGAGTTGTACAGCAAATAGCACCTCAAAATGGGCAGtg > 2:160612/1‑142 (MQ=255) aGCGCTCCAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACAGAGTTAAAATGTCAAGTTc > 1:459550/1‑143 (MQ=255) ccAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTTATCTACGAGGATCACGGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTCCAGTTCGTTTTc < 1:215968/143‑1 (MQ=255) cAGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATATCTTCCTCCCTGATCAACAAAGATAATTGATTCTCGATAATATCACTAACATTGTACTGGAAATACCGAGATAAAATGTCCAGTTCGTTTTCg > 1:294575/1‑143 (MQ=255) aGCGGCAATACCGCTTTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTATACTGGAAATACCGCGTTAAAATGTACAGTTTGTTTTCga > 1:256761/1‑142 (MQ=255) | AAGAAACAATCTTGCCGTCCTTCACTTCTACGTTCATCGCTCCCCAACGTCCCGCGGTGAGGATTTTACCGCCCTTCTCTTCTGCCCATGCGGGGAGCGGTGCTGCCGATGTCACCACCAGCGCTCCAGCGGCAATACCGCTGTGTTTAATAAATTCACGTCTTGTTAATGTCATAACTTCCTCCCTGATCAACGAGGATCACTGTTTCTCGGTAATATCTTTGGCGTTGTACTGGAAATACCGCGTTAAAATGTCCAGTTCGTTTTCGC > NC_000913/1956833‑1957102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |