| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,270,605 | G→A | 15.1% | intergenic (+60/‑121) | mepS → / → pdeN | peptidoglycan DD‑endopeptidase/peptidoglycan LD‑carboxypeptidase/putative c‑di‑GMP phosphodiesterase PdeN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,270,605 | 0 | G | A | 15.1% | 50.3 / 2.7 | 26 | intergenic (+60/‑121) | mepS/pdeN | peptidoglycan DD‑endopeptidase/peptidoglycan LD‑carboxypeptidase/putative c‑di‑GMP phosphodiesterase PdeN |
| Reads supporting (aligned to +/- strand): ref base G (11/11); new base A (2/2); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.54e-01 | |||||||||||
TGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGT > NC_000913/2270467‑2270729 | tgtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCTGGGTTCTCACCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGGCGCGTTAACTGCAAGCACTGCTTAGGCAg > 2:94046/1‑143 (MQ=255) ttattaTTTCTAGCATTAATGAACCGTACTGGAAGAAGCGTTATAACGAAGTATGTCTGGTTCTCAGCCGCAGCGAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTg < 1:18121/143‑1 (MQ=255) attTCCAGCATTAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGTGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCtttc < 1:460827/143‑2 (MQ=255) tttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCAGACCTTAGGCAGCGCttttt > 2:171447/1‑143 (MQ=255) ttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCTTTTTTTTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCtttttt < 1:122239/143‑1 (MQ=255) aGCATGAATGAACCGTAGGGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTGTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGttt < 2:46228/143‑1 (MQ=255) tatgaatgaACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGTAGCTAATTAACTGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTGAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttc < 1:267133/142‑1 (MQ=255) atgattgaACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGTAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttca < 2:368377/143‑1 (MQ=255) cacccGTACTGTAATAAGCTTTACAACGAAGCACGCCGCGTTCTCAGCCTCAGCTAATAAACCGTTTGTATGCAATCCCTTGGCTATCCTGACTAGTTAACTGAAATCACTGCTTAGGCAGTGCTTTTTTGTTTTCATtcatc < 2:333255/140‑1 (MQ=255) cGTATTGGAAGAAGCGTTACAACGAAGCACGCCGTGTTCTCAGCCGCAGTTAATATTTCGTTTGGATGCAATGCTTTGGCGATCCTGACGAGTTAACTGACAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCagag < 1:16406/143‑1 (MQ=255) aCTGGAAGAAGCGTTACAACGAAGCACGCCAAGTTATCAGCAACAGGTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCGTTTTTGTTTTCATTCATCAGAGaaa > 2:396273/1‑143 (MQ=255) aCGCAGCACGCCCGTTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCtt < 2:103975/143‑1 (MQ=255) gcagtcGGGTTCTGAGCCGCAGCTAATCAACCGTTTGGATGCAATCCTTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCa < 2:223605/138‑1 (MQ=255) gCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGc > 2:407324/1‑143 (MQ=255) gCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCGATAGTCTggaagtt > 2:275049/1‑138 (MQ=255) cGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGCCTATAGTCCGGTCATTGTTATGTTTTaaa > 2:135883/1‑143 (MQ=255) tGCAATCCCTTGGCTATCCTGACGAGTTAACTGAACGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCg > 1:380726/1‑143 (MQ=255) aaTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTaa > 1:187055/1‑143 (MQ=255) cccTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTGGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAttt < 1:136810/143‑1 (MQ=255) cTTGTCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCTCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCa < 2:492852/143‑1 (MQ=255) ttGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAAGCAGTGCTTTTTTGTTTTCATTTATCAGTGATAATTATGTTTCCTCGTCTTGATCCAGGCTATAGGCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg < 1:14429/143‑1 (MQ=255) cTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTAACTTTTAAATATTGTAGTAATTTCAGGaaa > 1:41738/1‑143 (MQ=255) gTTAACTGAAAGCACTGCTTGGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATCAACGGAATCa > 2:58573/1‑143 (MQ=255) acaaaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTTCGGTCATTGATATATTTTAAATATTGTCGTAATTTAAGGAAATTAACAAAATAATGt > 1:202900/6‑143 (MQ=255) acaaaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGACATTGTTATCTTTTAAATGTTGTCGTAATTTAAGGAAATTAAAGTAATCATGt > 1:202766/6‑143 (MQ=255) aaCTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTACGGTCAATGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGt > 2:49193/1‑143 (MQ=255) | TGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGT > NC_000913/2270467‑2270729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |