| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,398 | T→G | 100% | L29R (CTT→CGT) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,398 | 0 | T | G | 100.0% | 65.3 / ‑6.7 | 26 | L29R (CTT→CGT) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (14/11); total (14/12) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGT > NC_000913/3041267‑3041534 | ttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGATTCTATTATTGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTa < 1:452648/143‑1 (MQ=255) tACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTTTTCTCCGTCAGCCTACGGATTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTAc > 1:296723/1‑143 (MQ=255) cATTATTCATTTTTACGCCGTTAAGCATCTCAGGACTCTATTAGTGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCTTTTACCGCTTACGCTGATGACGCTTTATGACTGGGCGCGTGCCACCATTACTgg < 2:162375/143‑1 (MQ=255) tCATTAATACTCCGTTATGCACCTCATGGCTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa < 2:56220/143‑1 (MQ=255) aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACa > 1:488267/1‑143 (MQ=255) ttAATACGCCGTTAAGCAACTCAGGACTATATTATGGCTTTTACACCTTTTCCTCCCCGACAGCCTACAGCGTCTGCCCGTTTACCGCTGACGCTGATGACGCCTGAGGACTGGGCACGTGCCGCCATTACTCGCGCGGACAg > 2:412269/1‑143 (MQ=255) gCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaaaaa < 2:325931/143‑1 (MQ=255) ttgatgCATCTCCGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCGTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatat < 2:416703/138‑1 (MQ=255) ctATTCTGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACTGCTGACGCTGATGACGCTTGATGACTGGGCGCGGGCCACCAGTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAca < 1:199228/143‑1 (MQ=255) ttttACACCTTTTCCTCCCCGTCAGCCTACTGCTTCTGCTCGTTTATCGCTGACGCTTATGACGTTTGATGACTGGGCGCGTGCCAACATGACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa < 1:397959/143‑1 (MQ=255) acCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGa > 1:167532/1‑143 (MQ=255) cctgtcctccCCGTCGGCCTAGCGTTTCTGGCGGTTTACCGCTGACGCTGATGAGGCTTGATGACTGGGTTTTTGCAACCATTACTGGCGCGGACAGCGAAAAATATTTGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATg < 2:423735/139‑1 (MQ=255) cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGCTGGCGCTTGATGACTGGGCGCGTGCCACCATTACTGACACGAACAGCGAAACATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATg > 1:47744/1‑143 (MQ=255) cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCCGATGACGCTTGATGACTGGGCGCGTGACACCATTACTGGCGCGGACCGCGAAAAATATAAGCAGGGTCAGGTGACAGCAGACGTGAGACAGagg > 2:243115/1‑141 (MQ=255) tccCCGTCAGCCTACGGCTTCTGCCCGTTTATCTCTGACGCTTTTTACTCTTTTTTACTGGGCGCGTGCCACAATTACTAGCTTTTACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag < 1:168840/143‑1 (MQ=255) tcccGTCATCCTACGGCTTTTGCCCGTTTACGGATGACGCTGATGACTCTTGATGCCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGTAGGGTCAGTTGACAGCAGATGTCAGCCAGATGGCagaaga < 2:113239/142‑1 (MQ=255) cccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCCCCATTACTGGCGCGGCAAGCGAAAAATAGAGGCAGGGTCAGGGAACAGCAGATGACAGCCAGATAGCagacgca > 2:308311/1‑139 (MQ=255) cTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTg > 1:365487/1‑143 (MQ=255) cGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGTGGACAGCGAAAAATATACGCAGGGTCAGGTGACAGCAGATGTCGGCCAGATGGCAGAAGATCAGCACCTGCACGCCGCACAtca > 2:51536/1‑141 (MQ=255) tACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGAc < 2:167532/143‑1 (MQ=255) acgctgaTGACGCTTGATGCCTGCGCGCGTGCCACTATTTCTTGTGCGTACAGCGAAAAATTTATGCAGTGTCAGGTGACAGCAGATGGCAGGCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAgg < 1:240603/143‑1 (MQ=255) tgatgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaaa > 2:389275/1‑143 (MQ=255) atgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTAACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCACCCCACTGCGACACACAAGGTCAAAt > 2:50530/1‑143 (MQ=255) atgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGCGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCAAAAACGACGACAAAGATaaact > 1:78715/1‑141 (MQ=255) tGGGCGCGTGCCACCATTACTAGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATGGCGACGCCGCGGGAAAACCGTGGCGCAATTTACg > 2:358633/1‑143 (MQ=255) gggCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCAAAAGGTAAAATGTGGAACAATTTACga > 1:394819/1‑142 (MQ=255) | TTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGT > NC_000913/3041267‑3041534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |