| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,184,543 | C→A | 100% | P1100Q (CCG→CAG) | rpoB → | RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,184,543 | 0 | C | A | 100.0% | 97.7 / ‑6.7 | 42 | P1100Q (CCG→CAG) | rpoB | RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (25/15); total (26/16) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA > NC_000913/4184412‑4184681 | tAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc > 1:342346/1‑143 (MQ=255) cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCAAAGATCAACCCGATCGAAGATATGCCTTAACATGAAAACGGTACGCCGGAAGACATCGTACTGCAACAGCTCGGCGGAccatg > 2:289304/1‑140 (MQ=255) tCCAGACGGGTGAAAAGATGGCAGGTCGTCACGGTAACAAGGGTTTCATAACAAAAATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg < 1:209184/143‑1 (MQ=255) aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGCGTAATTTCTAATATCAACCCAATCAAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTCGGCGTACCGTCTCGTATGCAc > 1:466902/1‑143 (MQ=255) cggtggtaCAAGAGGGCAGGTCGTCACGGTAACAAGGTTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAt < 2:90311/136‑1 (MQ=255) ccTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACCGAACCCGCTGCGCGTACCGCCTCGTCAGAACAt > 2:58615/1‑143 (MQ=255) aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCCACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCAGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGGATGAACATCGGTCAGAt > 1:71389/1‑143 (MQ=255) aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt > 1:396330/1‑143 (MQ=255) aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGGCAGAt > 1:459047/1‑143 (MQ=255) tGGCAGTTCGTCACGTTAACGAGGGTGTAATTTCTAAGATCATCCCGATCGAAGATATGCCTTACTATGACAACGGTACGTCTGTAGACATCGTACTGTACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc < 2:452886/143‑1 (MQ=255) gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGCAGACATCGTACTGAACCAGCTGGGCGGACCGTCTCGTATGCACATCGGTCAGATCCCAGAAACCCAc > 2:41760/1‑143 (MQ=255) tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAACCGGTCCGCCCGTAGACATCGTACTGAACCAGCTAAGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAcc > 1:48753/1‑143 (MQ=255) cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAcat > 1:456974/1‑141 (MQ=255) aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTg > 2:162243/1‑143 (MQ=255) aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTg < 2:190090/143‑1 (MQ=255) cAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCCCGAAACCCACCTGGGTATgg > 1:466507/1‑143 (MQ=255) gtgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTAAAGTATGAACATCAGTCAGATACTCGAAAACAACATGGGTATGGCTGCg > 1:40010/1‑143 (MQ=255) gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaa > 2:236125/1‑143 (MQ=255) tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGACTCGAATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa > 1:254596/1‑143 (MQ=255) gccccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACaa < 2:486119/141‑1 (MQ=255) ccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTCAACCAACTAAACGTACAGTCACGAATGAACATCGGACAGATCCTCGAAAACCACATGGGTATGGCTGCGAAAAGTATCGGCGACAAGAt > 1:326222/1‑143 (MQ=255) gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGATATAGCTGCGAAAGGTATCGGCGACACGATCAACGc > 1:92590/1‑143 (MQ=255) gAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCATCGAAAACAAACAGGGTATGGCTGCGAAAGGAATAGGCGACAAGATCAACGc > 1:95688/1‑143 (MQ=255) ggagatGTCTTACGATCGAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGTGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa < 2:290565/139‑1 (MQ=255) aGATCTGTCTTACGATGAAAGCGGTACGCCGGTAGGCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa < 2:233085/143‑1 (MQ=255) atgtgtcTTACGATGAAAACGGTACGCCGGTAGATATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg < 1:305941/137‑1 (MQ=255) cTTACGATGAAAACGCGACGCCGGTAGACATCGTACTGAACCTGCTGGGTGTACTGTCTCGTATGAACATCGGTTAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa < 2:116223/143‑1 (MQ=255) gaCGATGAAACCGGTACGCCTGTAGACATCGTACTGAATCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGTCGACAAGATCAACGCCATGCTGAAAca < 2:44008/142‑1 (MQ=255) aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGAATGGCTGAGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa > 1:431300/1‑143 (MQ=255) gtccGCCGGTAGACATCGTACCGTACCAGCAGGGCGTCCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATCGCTGCGAAAGGTATCGGCGACAAGTTCAACGCCATGCTGAAACAGCAGCAAGAAGTc < 2:120870/140‑1 (MQ=255) tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTTGTATGAACATCGGTCAGATCCTCGAAACCCACCGAGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg < 1:236125/143‑1 (MQ=255) tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg < 2:342346/143‑1 (MQ=255) cGCCGGTAGACATCGTACTGAACCAGCTCACTGGGCGTTCTCGTATGAACATCGGTCAGATCCTCGAAACGCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgcg < 1:374064/143‑1 (MQ=255) ggTAGACATCGTACTGAACCAGCTGGGCGTCCGGTCTCGTATGTACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCTTGGTATCGGCGACAAGATCAACGCAATGCTGAAATAGCAGCAAGAAGACGCGAAAc > 1:192354/1‑143 (MQ=255) gTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGATAAAGAAGACAAGATCAACGCCATGCTAAAACAGCAGCAAGAAGACGCGAAACt > 1:185051/1‑143 (MQ=255) aGACATCGTCCTGAACCAGCTGGCCGTCCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc < 2:396330/143‑1 (MQ=255) gACATCGTGCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGCAACCCACCGGGAGATGGCTGCGAAAGGTATCGGCGACCCGATCCACGCCCTGCTGATACAGCAGCACGAACTCTCGACACTgcg > 1:83577/1‑143 (MQ=255) gACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATGCTCGAAACCCACCTGGGTATGGCTGCGAGAGGTATCGGCGACACGATAAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGcaaacgcg > 2:484966/1‑138 (MQ=255) cATCGTACTGAACCAGCCGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGcgcg < 2:40010/143‑1 (MQ=255) aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATAAACGCCATACAGAAAAAGCAACAAGAAGTCGCGACACTGCGCGa > 1:429477/1‑143 (MQ=255) aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCTACCTGGGTATGGCTGCCAACGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCACGAAGTAGCGAAACTGAGCGAATTCATACa > 2:268137/1‑143 (MQ=255) aaCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCAGCGACAAGATCAAAGCCATGCTGAAAAAGCAGCAAGAAGTCGCGAAACTACGCGAATTCATcaa > 1:222230/1‑141 (MQ=255) | TAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA > NC_000913/4184412‑4184681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |