| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 710,489 | G→C | 100% | A53G (GCT→GGT) | fur ← | DNA‑binding transcriptional dual regulator Fur |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 710,489 | 0 | G | C | 100.0% | 60.3 / ‑6.7 | 25 | A53G (GCT→GGT) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base C (14/8); minor base A (1/0); total (15/10) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAG > NC_000913/710350‑710615 | aaCCTTGCCTCTCTCGAGTCAGACGCGGTGATCGCGGTGATGTTTGTGTGCCAGTTCAAATACGGATTTACCGCCTTCAAAACTGTGGCGGGTGACGATACCAGCTTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCa < 2:125561/143‑1 (MQ=255) aGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGTTTTACCTCCTTCAAAATTGTAGCTGGTGACGATACCAGCGTCGTTAAACTTGTTCAGTACGCGATATACCATAACCAGACCAATTTCTTCAcc > 1:133629/1‑143 (MQ=255) aGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATATGTATTTACCGTCTTCAAAATTGTGGCTTGTGACGATACCAGCGTCGTCAAACTGGTGCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAt < 1:136080/143‑1 (MQ=255) gATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTTGTCAAACTGGTTAAGTACGCGATATACCGTACCCAGACCAATTTCTTCACGCATATCGAttgga > 1:295615/1‑139 (MQ=255) aTCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCAGACCCAGACCAATTTCTTCAACCACATGGACAAGAc > 2:323471/1‑143 (MQ=255) gtgATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGt > 1:356740/1‑143 (MQ=255) gtgtCAGTTCAAATACGGATTTACCACCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAACAAACATTTGTATAAATATTcc > 2:98452/1‑143 (MQ=255) tgtCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCg < 1:252410/143‑1 (MQ=255) tgtCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCg > 1:441994/1‑143 (MQ=255) gTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTATCCACCCAAATCGATCAGACGTTTGTATAAATCTTACGCACTg > 2:122675/1‑143 (MQ=255) tCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGAGCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGAc < 1:160540/143‑1 (MQ=255) cGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGGACCCAGACCAATTTCTTAACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACgagagga > 2:449481/1‑140 (MQ=255) ttACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTc < 2:356740/143‑1 (MQ=255) tgGCGGGTGAGGATACCGGCGTCGTCAAACTGCTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTc < 2:489972/143‑1 (MQ=255) tgGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATAGCGTACGCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAAGCTTCCGCACTGACGGGATGGTTGTACGGCGCTTGAAGAACtgc > 2:442541/1‑141 (MQ=255) tgatgaTACCAGCGTCGTCAAACTGGTTCATTACGTTTTATACCGTACCCAGAGCATTTTCTTCAACCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGGTGTCCGGCTCCTGAAGAACTTCCAGGAtt < 1:227859/143‑1 (MQ=255) gATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTa > 1:15353/1‑143 (MQ=255) tACCAGCGTCGTCAAACTGGTTTAGTACGCGATATATCGTATTTATTTCAATTTTTTCACTCATATCGATGAGAAGTGTGTATAAATCATCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTaaa < 1:114685/143‑1 (MQ=255) aaaCTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTa > 2:458224/1‑143 (MQ=255) aCTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTCACTTGCTGGCACCCCTGCTCCTAAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACt > 1:324804/1‑143 (MQ=255) tGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCTGACATTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCGCCGGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACttt < 1:383860/143‑1 (MQ=255) ttCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAg < 2:292115/143‑1 (MQ=255) aGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATATTCAGAACTGACATGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGcc > 1:106115/1‑143 (MQ=255) gTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCCCTGCCGTCATGGTTGTCCGGCTCCTAAAAAACATCCAGGATATTTAAACGAGGAAGCGTAACTTTCAGGCCa > 1:423489/1‑143 (MQ=255) tACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGCCGTTTGTATAAAACTTCCGCACTAACGTGAAGGAAATCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCTAg > 1:15542/1‑143 (MQ=255) | AACCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAG > NC_000913/710350‑710615 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |