Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,026,927 A→T 100% V30E (GTG→GAG)  mgsA ← methylglyoxal synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,026,9270AT98.8% 21.4 / ‑7.1 12V30E (GTG→GAG) mgsAmethylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (8/3);  minor base G (0/1);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAG  >  NC_000913/1026787‑1027045
                                                                                                                                            |                                                                                                                      
aTGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCACCATCGCGTTGACGCTCATGCCGGTCGCGCGGGAACTTAAGTTACCGGTAGTGACTGTTGCATACAGTACGTGTTGTTCCCGTAACTGTTCATGCCGTtcctcc                                                                                                                      >  1:428364/1‑143 (MQ=255)
                          aTTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCTCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTg                                                                                            >  2:284757/1‑143 (MQ=255)
                          aTTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACAAATAATGCAGTTCCTCCCAGCACATCAGAATTTGTTTGCAGTg                                                                                            >  1:208900/1‑143 (MQ=255)
                                ccACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAACTTCCGTTACCGCTAGTGCCTATTACATACAGTACGTGTTGTTCCAATAACGGTTGATGCCATTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCgtg                                                                                      >  1:386741/1‑143 (MQ=255)
                                    tCAACTTCGTGTTGACGTTCATGCAGGTCGCGCGGGAAATTGAGTTCCCGTTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGcc                                                                                  <  2:388534/143‑1 (MQ=255)
                                                     taaaTGACGGTCGCGCGTGCCATTAAGTGAGCGCTAGTGCTTGTGGAGTTCACTCTGTGTCGTTACGTTAATGGCTGTTGGTGTACCGCCCAACTCCTCAGAATTTGTGTGTAGGGATCGGGTGCCACCAGCGCAATATGttt                                                                 <  2:201813/140‑1 (MQ=255)
                                                            cGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCCCa                                                          >  2:280199/1‑143 (MQ=255)
                                                                        aaaTTAAGTTATCGTTAGTTCTTGTTGCATACAGTACGTGTTTTTTCATTTACGGTTGATGCCGTTCCTCTCAGCTCATCAGCATGTGCTTGCAGCGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGa                                              <  1:233824/143‑1 (MQ=255)
                                                                                ttACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTTATTCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCAACCAGCGCAATATATTTCAGCGCAGGTAAAGTGCCAGACGTCAg                                      >  1:126780/1‑143 (MQ=255)
                                                                                         gTGCCTTTTCCATACAGTGCGTGTTCTTCCAGTAATGGTTGTTGCCGTTCCTCCCACCTCATGAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAAt                             <  2:274971/143‑1 (MQ=255)
                                                                                              tGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCCCCATTTCTTTCCAGTGATCGTGTGCCACCAACAAAATATGATTCCGCGCAGGTAAAGTGCGAGACAACAGTTCCATAATGTACa                        >  1:399614/1‑143 (MQ=255)
                                                                                                         gTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTCGTTTGCAGTGCTCTTTTGCCACCACCCCAATATGCTTCCGCGAAGGAACAAAGCGAGTCATCAGATCCAAAATGAACATCCGAAATTaa             >  1:85368/1‑143 (MQ=255)
                                                                                                                    tCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATACGTAGATAACATTACAACAg  >  1:72862/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                      
ATGCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAG  >  NC_000913/1026787‑1027045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑