Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,196,220	C→T	100%	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	100.0%	24.0 / NA	15	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/7);  total (8/7)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGAT  >  NC_000913/1196080‑1196316                                                                                                                                             |                                                                                                 atcagtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCTCCCTCGGTATTGCGCTTAAATACGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCatat                                                                                                <  1:289278/143‑1 (MQ=255)         cgCCCCTGGTGCTAACTTCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGTAATATGCCGGTCAGGACAAAGTAATGCCGGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGa                                                                                        <  2:8760/143‑1 (MQ=255)          gCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTAGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGAc                                                                                       >  1:338735/1‑143 (MQ=255)                       aCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAc                                                                          <  1:48879/143‑1 (MQ=255)                             gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgatt                                                                    >  1:354510/1‑137 (MQ=255)                                   aaTGCGCCCTGGTTGAAGCCACCCACGGTACTGCGTTGATATATGTTGTTCAGGACATTGTTAATTCTGGCTCTATGATTCTCTCCGCTGGGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                                              <  1:402682/143‑1 (MQ=18)                                        gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAACGTAAATCCTGGATCTATTATTCTCTACGCTGAGATGATACTGCGCAATATGGGTTGGACTGAAGCGGCTGACCTGAATGTTAAAGGTAt                                                         >  1:57834/1‑143 (MQ=37)                                           cTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATTCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGATTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa                                                      >  1:236124/1‑143 (MQ=37)                                            tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGAACTGAAGAGGCTGACCTGATTGTTAAAGGTATGGaa                                                     >  1:289268/1‑143 (MQ=37)                                                    gCCACCCACGGTGCTTCGGCGAAATATGCCGGTCAGGTGAAAGTAAGTCCTGGCGCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAAt                                             <  2:131313/143‑1 (MQ=21)                                                           acGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGACGGCGCACTCAAcgcc                                      >  2:456215/1‑142 (MQ=25)                                                             tgTACTGCGCCGTAATATGCCGGTCAGGACAATGTAATTCGTGTCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaa                                    <  1:234541/142‑7 (MQ=18)                                                             ggTACTGCGCCGAAATATGCCCGTCAGGACAAAGTTATTTCTGGTTCTATTATTTTCTCTTCTGTGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaa                                    <  1:424646/143‑7 (MQ=17)                                                                       cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGAAAGGCGCAATCAATGCCAAGACCGTAAct                          >  2:13733/1‑142 (MQ=18)                                                                                        gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt         >  2:98627/1‑143 (MQ=11)                                                                                               tAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTTGATTGAAGCTGCTGTCTTTTTTGTTATAGGTATGGAAGGCGCAATCAAAGCCAAGACCGTAACTTATGACTTCGAACGTCTgatggaa  <  1:6129/143‑2 (MQ=14)                                                                                                                                             |                                                                                                 ATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGAT  >  NC_000913/1196080‑1196316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑