| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,196,247 | A→G | 100% | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,196,247 | 0 | A | G | 100.0% | 16.5 / ‑6.7 | 11 | L375M (TTA→CTG) | icd | isocitrate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (6/4); minor base T (0/1); total (6/5) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGAT > NC_000913/1196114‑1196316 | aaTGCGCCCTGGTTGAAGCCACCCACGGTACTGCGTTGATATATGTTGTTCAGGACATTGTTAATTCTGGCTCTATGATTCTCTCCGCTGGGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa < 1:402682/143‑1 (MQ=18) gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAACGTAAATCCTGGATCTATTATTCTCTACGCTGAGATGATACTGCGCAATATGGGTTGGACTGAAGCGGCTGACCTGAATGTTAAAGGTAt > 1:57834/1‑143 (MQ=37) cTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATTCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGATTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa > 1:236124/1‑143 (MQ=37) tGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGAACTGAAGAGGCTGACCTGATTGTTAAAGGTATGGaa > 1:289268/1‑143 (MQ=37) gCCACCCACGGTGCTTCGGCGAAATATGCCGGTCAGGTGAAAGTAAGTCCTGGCGCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAAt < 2:131313/143‑1 (MQ=21) acGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGACGGCGCACTCAAcgcc > 2:456215/1‑142 (MQ=25) tgTACTGCGCCGTAATATGCCGGTCAGGACAATGTAATTCGTGTCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaa < 1:234541/142‑7 (MQ=18) ggTACTGCGCCGAAATATGCCCGTCAGGACAAAGTTATTTCTGGTTCTATTATTTTCTCTTCTGTGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaa < 1:424646/143‑7 (MQ=17) cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGAAAGGCGCAATCAATGCCAAGACCGTAAct > 2:13733/1‑142 (MQ=18) gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt > 2:98627/1‑143 (MQ=11) tAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTTGATTGAAGCTGCTGTCTTTTTTGTTATAGGTATGGAAGGCGCAATCAAAGCCAAGACCGTAACTTATGACTTCGAACGTCTgatggaa < 1:6129/143‑2 (MQ=14) | AATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGAT > NC_000913/1196114‑1196316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |