| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,940,584 | C→A | R351L (CGT→CTT) | mepM ← | peptidoglycan DD‑endopeptidase MepM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,940,584 | 0 | C | A | 91.1% | 122.2 / NA | 47 | R351L (CGT→CTT) | mepM | peptidoglycan DD‑endopeptidase MepM |
| Reads supporting (aligned to +/- strand): ref base C (4/0); new base A (21/20); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATT > NC_000913/1940445‑1940723 | ttCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGCTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGCTGTAGCTGCGACCAT‑GCCGaa > 1:212425/1‑143 (MQ=255) tCAGAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAAt < 1:724397/143‑1 (MQ=255) tGCAGATGCGGCCCGGTTGAACGTCCGGTATTATCGGAAAGCGCGATACGGTCGCCACGTTTCACCATCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAGGAATAGCcac > 1:677916/1‑143 (MQ=255) cAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACat < 1:788666/143‑1 (MQ=255) gATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGGGCGGTACGGTAGGGACGAAACAAAGAATGCAATCTGCTGACGATGAGCTTGCGCAAGTGCTTATCACGCGTGGTCTAGCTGCGACCAT‑GCAGAATAGCCACataa > 1:241052/1‑143 (MQ=255) gATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACataa < 1:178177/143‑1 (MQ=255) aTGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGCATCTTGCGCAAGTGCATCTAACGCGTGGTGGAGCAGCGCCCATGGACG‑ATACCCACataag > 2:761399/1‑142 (MQ=255) gCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGGCCCGGTTTGACCAGAATCTTGCGCAAGGGCATATAACGCGTGGTGTAGCTGCGCCCAT‑GAAGAATAGCCACAtaataa > 1:730551/1‑143 (MQ=255) ggCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAAcc < 1:785579/143‑1 (MQ=255) ccccGTTTGACCGTCCGGTCTTACCGGAACGCGCGTGACGGTCGCCACGTTTCACCTTCTGTCCCGATTTCACCAGATTCTTGCGCAAGTGCATATAACTCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACct < 2:871289/142‑1 (MQ=255) cGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGGGTGGGGTAGCTGCGGCCAT‑GAAGAATAGCCACAAGATAACctgct > 1:194975/1‑143 (MQ=255) tCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGc > 1:728354/1‑143 (MQ=255) ggtttACCGGAAGGCGCGGTACGGTCGCCGCGTTTCACCGTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGttt < 2:321093/140‑1 (MQ=255) aTTACCGGACAGCGCCATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGCATCTCGCGCAAGTCCATATAACGCGTGGTGTAGATGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTgg < 1:73495/143‑1 (MQ=255) ccGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAac < 2:871715/143‑1 (MQ=255) cGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCCCCAGAATCTTGCGCAAGTGCATATACCCCGTGGTGTCGCCGCGACCAC‑GCACCCTAGCCACCTAATCACCTGCTGCCCCACTCCGTCTCGCGacc > 1:886218/1‑143 (MQ=255) cGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCACATAACGCGTGGTGTAGCTGCGACCAT‑GACGAATAGCCACATAATAACCGGCTGCGCCGCTGCGGCTCGCAacc > 2:424803/1‑143 (MQ=255) cGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTATCCCGGTTTCACCAGAATCTTGCGGAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTCAGGTTGGCCacc > 1:271094/1‑143 (MQ=255) gcgATACGGTCGCCACGTTTCACCTTGTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTAGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTc < 2:668644/143‑1 (MQ=255) tttCACCTTCTGTCCCTGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGaaa < 1:542557/143‑1 (MQ=255) tttCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAACTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTCGCAACCCCCACTCCACCGTCCCCCCCTGaca > 2:448564/1‑141 (MQ=255) tttCACCTTCTGTCCCGGTTTCACCACACTCTTGCGCAACTGCCTATAACGCCTGGTGTAGCTGCCACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACCGCGTCTGGCACCCACCACTTCCCCCTCACCCCCTgcca > 2:85079/1‑140 (MQ=255) tttCACCGTCTGTCCCGGTTTCACCAGAAGCGTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGaaa < 1:526636/143‑1 (MQ=255) cACGTTCTGCCCCGGTTTCACCAGAACTTTCCCCAAATGCATCTAACGTGTGGTGTAGCTGCGCCCAT‑GAGGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCa < 2:675066/143‑1 (MQ=255) aCCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACGGCGGTTGGCAACCACCACTTCACCGTCACCGCCTGAACGCAc > 1:225788/1‑143 (MQ=255) cgtCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTg < 1:196628/141‑1 (MQ=255) tCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACATGCTGCGACACGACGCCTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTAc > 1:235903/1‑143 (MQ=255) cACCAGAGTGGTGCGGAAGTGTATAGGACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGGCACATAATAACCTGTTGCGCGACTGCGTGTGGCGACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCa < 2:682989/143‑1 (MQ=255) aCCAGAATCTTGCGCAGGTGCATATAAGGCCTTGTTTAGCTGGGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGTCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCAt < 2:212425/143‑1 (MQ=255) aCCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCAt > 1:681131/1‑143 (MQ=255) aCCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCAt > 1:211029/1‑143 (MQ=255) aCCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCCGCGGCAt > 1:380088/1‑143 (MQ=255) cAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTAACCCACGGAAAGCACTGGCGTACCCTGCGGCATgg > 2:777134/1‑143 (MQ=255) cAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCAAATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCAACAACTGAAAGCACTGGCGTACCCTGCGGCATgg > 1:107802/1‑143 (MQ=255) gAATCGTGCGCAAGTGCAGATCACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACCTAATAACCTGCTGCGCCACTGCGTGTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCg < 2:701313/143‑1 (MQ=255) tCTTGCGCAAGTGCATATAACACGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCCTGGCGaaa > 1:898510/1‑143 (MQ=255) ggcgcAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCa < 1:386035/142‑1 (MQ=255) gcAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCTCTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAaca < 1:595000/143‑1 (MQ=255) cAAGTGCATATAACGCGTGGTGCAGCTGCGACCAT‑GAAGAATAGCCACATACTAACCTGCTGCGCCACTGCGTTTGGCAACCACCACGTCACCGTCACCCCCTGAAAGCACAGCCGTACCCTGCTGCATGGCGAAATCAacac < 1:150300/143‑1 (MQ=255) tGCATATAACGCGTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACctct < 1:490106/143‑1 (MQ=255) cATATAACGCGTGGTGTAGCTGCGACCAT‑GACGAATAGCCACACAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGTGTACCCTGCGGCATGGCGAAACCATCACCTCcgt > 2:550066/1‑143 (MQ=255) gcgTGGTGTAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCa > 1:849981/1‑143 (MQ=255) gtgtAGCTGCGACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGGCATGGCCACATCAACACCGCTGCGTGGAGCAGcgcg > 2:466981/1‑143 (MQ=255) gACCAT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGCACCCTGCGCCCCGGCGCAATCAGCACCACCGACTCGTGCAACGCCACCggacccc > 2:253901/1‑138 (MQ=255) cAT‑GAAGGATAGCCACATAATACCCTGCTGCGCCACTGCGTTTGGCAACCACCGCTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGa < 2:235903/143‑1 (MQ=255) aT‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGAt > 1:374110/1‑143 (MQ=255) t‑GAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCGGTGTGGTGCAACGCGCCCGGTCACCGGAtt > 1:743201/1‑143 (MQ=255) | TTCATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCAT‑GACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGATT > NC_000913/1940445‑1940723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |