Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,416,239	C→T	T498M (ACG→ATG)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,416,239	0	C	T	100.0%	160.8 / NA	54	T498M (ACG→ATG)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/25);  total (29/25)

ACTGGGTGCAGGGATTGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCATGCT  >  NC_000913/2416099‑2416377                                                                                                                                             |                                                                                                                                           aCTGGGAGCAGAGATGGAAAGAGTTGAGGCAGAAGGGGTGCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGc                                                                                                                                          <  1:393671/143‑1 (MQ=255)   tGGGTGCAGGGATTGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACGGCGTCAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGGAGACAACGTGGTGCTCGGTATGCTg                                                                                                                                        >  2:257639/1‑143 (MQ=255)     ggTGCAGGGATTGAAATCGTTGATCCAGAAGTGGTTCGCGAATGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTACCCGCGCACAGCTGGAAGACAACGCGGTGCTCGGTATGCTGAt                                                                                                                                      >  1:394061/1‑143 (MQ=255)           gggATTGAAAGCGTTGATCCAGAAGCGGTTCGCGAAAGCTATGTGGGTCGTCTGGTCGAACTGCGGAAGAATAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGa                                                                                                                                <  1:10161/143‑1 (MQ=255)              aTTGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACa                                                                                                                             >  1:422136/1‑143 (MQ=255)                    aTCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGa                                                                                                                       >  1:954431/1‑143 (MQ=255)                         tGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTg                                                                                                                  <  2:461158/143‑1 (MQ=255)                            tCCAGAAGTGGGCGGCGAAAGCTTTGCTGGTCGTCTGGTCGAACTGCGGAGAGACAAAGGCATGGCCGAAGCCGTTGCCTGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATg                                                                                                               <  2:310120/143‑1 (MQ=255)                             ccAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCGCGGTATGCTGATGCTGGAACAGGGCGAAGTTGATgg                                                                                                              >  2:328690/1‑143 (MQ=255)                               aGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGACGACAACGTGGAGCTCGGTATGCTGATGCTGGAACAGGATGAACTTGacagtc                                                                                                            >  2:94336/1‑138 (MQ=255)                                    gggTGCGCGAAAGCTACGTTAGTCGTCGTGTCGAACTGCGTAAGAACGAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGtt                                                                                                       <  1:457409/142‑1 (MQ=255)                                      gTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACCAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTc                                                                                                     <  1:234999/143‑1 (MQ=255)                                           cgAAAGCTATGTTGGTCGTCTGGTCGGACTGCGTAAGAACAAAGGCATGACCGAATCCGTTGCCCGCGAACAGTTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTg                                                                                                <  2:639981/143‑1 (MQ=255)                                                  tgtGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTca                                                                                         <  2:970333/141‑1 (MQ=255)                                                  tATGTTGTTCGTCTGGTCGAAGCGGGTAAGAACAAAGGCGTGCCCGAACCCGTTGCGCGCGACCAGGTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTca                                                                                         <  2:826553/143‑1 (MQ=255)                                                     gTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCAGGAACGGGATGAAGTTGATGGGCTGGTTTCCGGAGCGGTTcaccc                                                                                      >  2:569221/1‑141 (MQ=255)                                                      ttGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCGCTTCACACt                                                                                     >  2:293189/1‑143 (MQ=255)                                                        ggTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTAc                                                                                   <  1:115721/143‑1 (MQ=255)                                                         gtcgcatGGTCGAACTACGCAAGAACAAGGGCATGGCAACGACCTCTGCCCGCGATCAGCTGGAAGACAACGTGGTGCTCGGTCTGCTGATGCTGGAACAGGATGAAGTTGATTGTCTGGTTTCCGGTGCTGTTCACACTAcc                                                                                  <  1:87041/137‑1 (MQ=255)                                                                ggTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGAGCTGTTCACACCCCCGCAAaca                                                                           >  2:872585/1‑143 (MQ=255)                                                                   cGAACTGCCGAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCa                                                                        <  1:942650/143‑1 (MQ=255)                                                                   cGAACCGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCa                                                                        <  1:781678/143‑1 (MQ=255)                                                                          cGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGACGTTGATGGACTGGTTTCCGGTGCTGTTCACACTACCGCAAAGACGAtccgtcc                                                                 >  1:90077/1‑143 (MQ=255)                                                                          cGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCAtccgtcc                                                                 >  1:761138/1‑143 (MQ=255)                                                                           gTAAGCACACAGGCGTGACCGATAGGGTTGCCCGCGCATAGCTGGAAGACCGCGTGGTGCTCGGTATGCTGGTGCTGGAACAGGATGAAGTTGTTGGTCTGGTTTCCGGGGCTGTTCACACTTCCGCAAACACCAtccgtccg                                                                <  2:124949/143‑1 (MQ=255)                                                                            tAAGAACAAAGGCATGACCGAAACAGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCAGATGCTGGAACAGGATGAGGTTGATGGTCTGGTTTCCGGTCCTGTTCACAGTACCGCAAACGCCATCCGTccgc                                                               >  2:811905/1‑143 (MQ=255)                                                                              aGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACGACCGCAACCACCATCCGTccgccg                                                             >  2:105401/1‑143 (MQ=255)                                                                               gAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGGAGTTGATGGTCGGGTTTGCGGTGCTGTTCACCCTACAGCCAACACCATCCGTacgcccc                                                            >  2:510262/1‑141 (MQ=255)                                                                                 aCAAAGGCATGACCGAAACCATTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGACGTTGATGGTCTGGTTTCCGGTGCCGTTCACACTAACGCAAACACCATCCGTCCGCCGCtc                                                          >  2:89767/1‑142 (MQ=255)                                                                                   aaaGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATACTGATGCTGGAACAGGATGAAGTTGATGGTCCCGTTTCGGGGGCTGTGCACACTCCCGCAAACACCATCCGTCcgccgccgcc                                                        >  1:86848/1‑142 (MQ=255)                                                                                    aaGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAg                                                       <  1:654881/143‑1 (MQ=255)                                                                                       gCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATACTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCGGTTCACACTACCGCACACACCATCCGTCCGCCGCCGCAGCTg                                                    >  1:458287/1‑143 (MQ=255)                                                                                        cATGACCGAACCCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGa                                                   <  2:376384/143‑1 (MQ=255)                                                                                         aTGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGAt                                                  <  2:522873/143‑1 (MQ=255)                                                                                                 agccGTGGGCCGCGGGCAGCTGGAAGACAGCGGGGTGCTCGGTATGCTGGTGCTGGAACAGGATGAAGTGGATGGTTTGGTTTCCGGTGCGGTTCACACTACCGCCAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTg                                          <  2:268895/141‑1 (MQ=255)                                                                                                    cGTTGCCCGCGACCAGCTTGAAGACAACGTGGTGCTCGGTATGCTGCTGCTGGAAGAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCAGACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGTTAAAAACTGcca                                       >  2:418834/1‑141 (MQ=255)                                                                                                        gCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGccctcgg                                   <  2:422136/143‑7 (MQ=255)                                                                                                          ccGCGAACAGCTGGAAGACAACGTGGTGCTCGGTCTGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAACACTGCACCGGCCa                                 >  1:372541/1‑143 (MQ=255)                                                                                                           cgcgAATAGCCGGGAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAg                                <  2:608849/143‑1 (MQ=255)                                                                                                              gAACAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTc                             >  2:921508/1‑143 (MQ=255)                                                                                                               aaCAGCTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTcc                            <  1:322620/143‑1 (MQ=255)                                                                                                                 taGCGGGAAGACAACGTGGTTTTCGGTATGCTGATGCTGTAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCt                          <  2:333882/142‑1 (MQ=255)                                                                                                                    cTGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGt                       >  1:551047/1‑143 (MQ=255)                                                                                                                     tGGAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCCGCTGATCACAACTGCACCGGGCCGCTCCCTGGtc                      >  1:815480/1‑142 (MQ=255)                                                                                                                       gAAGACAACGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCCGCTGATCAAAACTGCCCCGGGCAGCTCCCTGGCATc                    >  2:1024079/1‑143 (MQ=255)                                                                                                                             gccGTGGTGCTCGGTATGCTTATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCgt              <  2:710356/141‑1 (MQ=255)                                                                                                                             aaCGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCgt              >  1:24214/1‑143 (MQ=255)                                                                                                                             aaCGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCgt              >  2:199521/1‑143 (MQ=255)                                                                                                                               cGTGGTGCTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCgtgt            <  1:764432/143‑1 (MQ=255)                                                                                                                                      cTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTAt                     <  1:513276/127‑1 (MQ=255)                                                                                                                                      cTCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTAt                     >  2:513276/1‑127 (MQ=255)                                                                                                                                       tCGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCGGCAGCTGATCAAAACTGCAGCGGGCAGCTCCCTGGGGTCTTCAGTGTTCTTCAtg    >  2:392383/1‑143 (MQ=255)                                                                                                                                        cGGTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACCCCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAtcc   >  2:161636/1‑141 (MQ=255)                                                                                                                                         ggTATGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAACACTGCACCGGGCAGCCCCCAGGTATCCCCCGTGTTCTACAtgcc  >  2:251762/1‑142 (MQ=255)                                                                                                                                             |                                                                                                                                           ACTGGGTGCAGGGATTGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCATGCT  >  NC_000913/2416099‑2416377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑