| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 710,489 | G→C | A53G (GCT→GGT) | fur ← | DNA‑binding transcriptional dual regulator Fur |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 710,489 | 0 | G | C | 97.7% | 125.2 / NA | 43 | A53G (GCT→GGT) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (25/17); total (25/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGG > NC_000913/710352‑710626 | tcTTGCCGCAGGCGAGGCAGATAGGGTGAGCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATGGTGGCGGGTGACGATGCCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGa < 2:247505/142‑1 (MQ=255) cGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTGGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAAtt > 1:712933/1‑143 (MQ=255) tggAGGCAGATCAGGTGATCGGGGTGAGGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATttcttc < 1:679605/141‑1 (MQ=255) tCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCACTttcttc > 2:78805/1‑143 (MQ=255) aGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGCCCAATTTCTTCAcc > 1:816132/1‑143 (MQ=255) ggCAGATCACGTGATCGTGGTGATGTGGCTGGGTCAGTTCAAATACGGATTTAACGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACTCAGACCAATTTCTTCAccc < 1:615554/143‑1 (MQ=255) gCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCa > 1:365494/1‑143 (MQ=255) tCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGAGTTACCGCCTTCAAAATGGTGGCGGGTGGCGATACCAGCGTCGTCAAACAGGTTCAGCACCCGCCATACCGTACCCAAACCACTTTCATCACCCATACCg > 2:59918/1‑143 (MQ=255) aGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAt < 1:26009/143‑1 (MQ=255) ggTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATc < 2:131768/143‑1 (MQ=255) aTCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCCGAc > 2:756126/1‑143 (MQ=255) cGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGt < 1:42443/143‑1 (MQ=255) cGTGGCGATGTTGCTCTGTCAGTTCAAACACGGATTTACCGCTTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGt < 1:36676/143‑1 (MQ=255) tGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATaa < 1:518779/143‑1 (MQ=255) ttGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATCTCGATCAGACGTTTGTATAAAt > 1:957391/1‑143 (MQ=255) tCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCCATATACCGTACCCCGACCAATTTCTTCACCCATATCGATCAGACGCTTGTACCAATCCTCCGCa > 2:834390/1‑143 (MQ=255) aGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAGATATTCCGCACt > 2:847651/1‑143 (MQ=255) gTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATCTACCGTACCCAGACCAATTTCTGCACCCATATCGAGCAGACGATTGTATAAATCTTCCGCACTg > 2:403511/1‑143 (MQ=255) gTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTg > 1:823905/1‑143 (MQ=255) ttCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGCTTGTATAAATCTTCCGCCCTGa > 1:496528/1‑143 (MQ=255) aTACGGATTTACCGCCTTCAAAATTGTGGCGGGCGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGCCCAATTTCTTCACCCATATCGATCAGACGCCTGTAGCAATCTTCCCCACTGACGCGa > 2:510466/1‑143 (MQ=255) aCGGATTTACCGCCTTCATAATGGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATg < 1:687194/143‑1 (MQ=255) aCGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACAGTACCCAGACCAATGTCTTCCCCCATATCGATCATACGTTTGTACAAATTTTGCGCCCCAACgtggtg > 2:928109/1‑143 (MQ=255) aCGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACAAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATAGATCCGCCGTCTGTACAAATTTTCAGCACGGACGTGCTg > 2:95221/1‑143 (MQ=255) ggATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCCATTTCTTCCCCCATATCTATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATAGt > 1:2573/1‑143 (MQ=255) tACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTcc < 1:623005/143‑1 (MQ=255) cccccTTCAAAATTGTGGCGGGTGACGATACCCGCGTCGTCAAACTGGTTCAGCACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCgg < 2:320248/140‑1 (MQ=255) cGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATAGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGc > 2:616180/1‑143 (MQ=255) ttCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCt < 1:988497/143‑1 (MQ=255) tcccaaaTTGTGGCTGGTTCCGATATCAGTGTTGCTAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATCTCGATCAGACGTTTGTATAAATCTTCCGCTCTGACGTGATGGTTGTCCGGCTCCt < 2:709990/139‑1 (MQ=255) ttGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTCGTATGGGCCCTGAAAAAc > 2:766173/1‑143 (MQ=255) ggTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCACTTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGa > 2:370078/1‑143 (MQ=255) aCGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGAtttt > 1:1011683/1‑143 (MQ=255) aCCTGCGTCGTCAATCTTGTTCAGTACGCGTTATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGTCTCCTGAAGAACTTCCAGGATTTTTAAAc < 2:584213/143‑1 (MQ=255) cAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACGTCCAGGATTTTTAAACGa > 2:584670/1‑143 (MQ=255) tcgtcAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGAGATGGTTGTCCGGCTCCTGAAGAACATCCAGGATTTTTAAACGAGGAAg > 2:11108/1‑143 (MQ=255) tcAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGt > 1:858229/1‑143 (MQ=255) tGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGAGTTTTACACGAGGAAGCGTTACttt > 2:234338/1‑143 (MQ=255) ttCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAg < 1:769954/143‑1 (MQ=255) cgATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGcttt < 1:259024/143‑1 (MQ=255) tataCCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGctttctt < 1:616180/143‑1 (MQ=255) tgctgTAGGCAGATCAATTTCTTCACCCGTGGCGTTCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGCCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTa < 2:653582/139‑1 (MQ=255) ccGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAgg > 1:352601/1‑143 (MQ=255) | CCTTGCCGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAGG > NC_000913/710352‑710626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |