| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,085 | Δ1 bp | 100% | coding (2069/2664 nt) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | . | 95.0% | 74.6 / ‑2.8 | 20 | coding (2069/2664 nt) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (9/10); minor base C (0/1); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124958‑125221 | actCAGTCGCTCACTATCCCGAACTGTATCTCTCACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGaa < 1:270525/141‑1 (MQ=255)aTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGaa > 2:24949/1‑143 (MQ=255) tCAGTCGCTGCCCATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGaaaa < 1:149474/143‑1 (MQ=255) tCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTATGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGaaaa < 2:142176/143‑1 (MQ=255) cgTCGCTTACTATCCCGAACTGTATCTCTCACGACTCGGCTTACGCTTACGAAGTTGCTGGCATCATGCCTGACGCTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACt < 2:451006/142‑1 (MQ=255) cTATCCCGAACTGTAGCTCTTGCGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGc < 2:172008/143‑1 (MQ=255) cTGTATCTCTTAGTACCCGGCTTCAGCGTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGcc < 2:226124/143‑1 (MQ=255) tGTATCTCTCACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCg < 1:51559/143‑1 (MQ=255) tctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCAAGCATGACCGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACCACTACA‑CACTAGGCTGAACGAAAGCTACAACGTGCCGGCAAGGCGGGAAggg > 2:248678/1‑142 (MQ=255) cGGCTTGCGCTTAGGATGTGGCTGTCATCATGCATGACGGGCTGGAGCGTATGTACGGTGAAAAACGAGATAACGGTTCCTACTCCA‑CACTACGCGGAACGGAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAg < 2:195710/143‑1 (MQ=255) tacgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTaac > 2:354145/1‑141 (MQ=255) acgcttacgGAGTTGCTCGTATCATACATGACGTTCTGGATCGTATGTACTGTGAAAAACAAGAGAACGTTTACTACTAC‑CCACTGCGCGGAACGATAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATcc < 2:307069/143‑1 (MQ=255) ttGCTGTCATCATGCATGACGGTCTGGAGCGTAGGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTAAGCGGAACGAAAACTACCACATGCCGGCAATGCCGGAAGCGGCTGAGGGCGGTCTCAGTAAAgcgacct > 2:424102/1‑137 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAACACAAGAGCACGTTTACTCCTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGCAGGCGCCGAGGCAGGTATCCGTCAAGGCATCTAcga > 2:320408/1‑141 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaa > 1:343903/1‑143 (MQ=255) tcatcaTGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAAc > 1:117119/1‑143 (MQ=255) catgcatgACGGTCTGGAGCGTATGCACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACGACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGa < 1:120995/143‑1 (MQ=255) aCGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGACGGTGCTGAGGAAGGTACCCGTCAAGGCATCTACAAACTCGAAACTacag > 2:318522/1‑140 (MQ=255) cgTCTGGAGCGTGTGGACGGTGAAAAACAAGAGAACGTGTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGaa < 2:29798/142‑1 (MQ=255) tactacA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGCAGGTCGCAAAGGCAAAGTTCAGCTGCTCGGCTCCGGTtctctc > 2:239413/1‑143 (MQ=255) | ATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124958‑125221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |