| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,785,179 | A→T | 100% | I645N (ATC→AAC) | ppsA ← | phosphoenolpyruvate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,785,179 | 0 | A | T | 75.4% | 33.3 / 5.4 | 20 | I645N (ATC→AAC) | ppsA | phosphoenolpyruvate synthetase |
| Reads supporting (aligned to +/- strand): ref base A (1/4); new base T (4/11); total (5/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACA‑GTCGCGGAAGCTGTCGGAAACATAGCGGCCCGCGCCACGGAAGCCGAGCATCGGGTTCTCTTCAT > NC_000913/1785045‑1785317 | gCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGTTCATGAtc < 1:454273/143‑1 (MQ=255) ggCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCaa < 1:4495/143‑1 (MQ=255) ggctagtcGTTGGACGGGACTTCACTCCTCCTGCTGATTTTCAGCCCTTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGtt < 2:389868/136‑1 (MQ=255) cgcccggCGTTGGCCGGGATTTCCCACCTCATGCTGCTTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGtt < 2:351756/138‑1 (MQ=255) agccGTTGGACGGGATTCCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGCTCGTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGTTGGTc < 2:347007/140‑1 (MQ=255) ggACCGGACTTCACACATCATGCTGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGTTGGTCAGTCCCa < 1:271484/143‑1 (MQ=255) gcgcgtgcacacaTCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTc < 1:241993/136‑1 (MQ=255) catGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGtt > 1:399079/1‑143 (MQ=255) gttgatTTGCCGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCa < 2:23418/141‑1 (MQ=255) ctcactttCCGCCCGTTCCCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCAc < 2:336515/138‑1 (MQ=255) gactttCCGCCCGTTCTCGCCACGTCTCCGCCCCTGCCGCGCCAGCTCTCCAACCACCGCTGTCGCCTGACCTCCGGTACGCACGAGCGGGTTCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTg < 2:263800/140‑1 (MQ=255) cACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGaaa < 1:461879/143‑1 (MQ=255) gTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACA‑GTCGCGGAAGCTGTCGGAAACa < 1:348964/143‑1 (MQ=255) tttCGCCTGATCTACGGTACGCACGAACGGGTTCATGCTCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGGTTCACTGCCTCACACTCCAGCGCGACACA‑GTCGCGGCAGCTGTCTGAACCATAAAGGACCGAGCCAc > 2:223722/1‑143 (MQ=255) ccTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACA‑GTCGCGGAAGCTGTCGGAAACATAGCGGCCCGCGCCACGGAAg > 1:276894/1‑143 (MQ=255) ccTGATCTACGGTACGCACGAACGGGTTCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACA‑GTCGCGGAAGCTGTCGGAAACATAGCGGCCCGCGCCACGGAAg > 1:274847/1‑143 (MQ=255) tCTACGGTACGCACGAACGGGTTCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACA‑GTCGCGGAAGCTGTCGGAAACATAGCGGCCCGCGCCACGGAAGCCGAg < 1:442019/143‑1 (MQ=255) aCGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGATACCCACTCCAGCGC‑AAACATAATGCTCAAGCTGCAGGTACCCTAGCGGCCCACCCCACGAAAGcagcacaa > 2:101417/1‑136 (MQ=255) cACGAACGGGAGCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACA‑GTCGCGGAAGCTGTCGGAAACATAGCGGCCCGCGCCACGGAAGCCGAGCATCGGGTtct < 2:353595/143‑1 (MQ=255) cGGGAGCATGAGCTCAACGGTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACA‑GTCGCGGAAGCTGTCGGAAACATAGCGGCCCGCGCCACGGAAGCCGAGCATCGGGTTCTCTTCAt < 1:344649/143‑1 (MQ=255) | GCTCGGCCAGCAAGGCGTTGGACGGGATTTCACACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGGATCATGATCTCAACGTTGGTCAGTCCCATGTCGTTGCGCACACGTTTCACTGCTTCACACTCCAGCGCGAAACA‑GTCGCGGAAGCTGTCGGAAACATAGCGGCCCGCGCCACGGAAGCCGAGCATCGGGTTCTCTTCAT > NC_000913/1785045‑1785317 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |