| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,789,616 | C→A | 75.4% | R2S (CGT→AGT) | ydiE → | PF10636 family protein YdiE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,789,616 | 0 | C | A | 75.4% | 37.2 / 5.1 | 25 | R2S (CGT→AGT) | ydiE | PF10636 family protein YdiE |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base A (13/5); total (16/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGC > NC_000913/1789480‑1789751 | gAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTtata < 1:157106/143‑1 (MQ=255) cccATTTCTGACGGAATGGGCATTTGTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCa < 2:30215/143‑1 (MQ=255) ccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCGATTATTATTAATTTTTATGAGTTATACGGATAGCAg < 1:314904/143‑1 (MQ=255) tCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGaaaa < 2:178330/143‑1 (MQ=255) gACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTATTTTTTATGAGTTATACGGATAGCCGAAAACTcc > 1:216690/1‑142 (MQ=255) gggCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGaa < 2:73351/143‑1 (MQ=255) tgtCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGa < 2:370937/141‑1 (MQ=255) gcgcAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAAAGGATGcga > 1:400964/1‑141 (MQ=255) gcgcAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAAATTTCAGTTATCGGACGGTTAGCAGAGGACTCACGCCTGAAACGGATGCCa > 1:220735/1‑143 (MQ=255) ttgttgTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGCATGCCAATCACaa > 2:79027/1‑143 (MQ=255) tgtCTTATCATCATATTTCTGTTGGCTCTGGTCTGCTAGAATATTGATAATATGAATCATTGTTATATTAATTTTTATTAATTTTAATGCGTCATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCTAATCACAAGAcc < 2:26504/143‑1 (MQ=255) ttctCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGCAACGGAGGCCAATCACAAGACCGCtc > 2:284609/1‑142 (MQ=255) ttctCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCtt > 2:116038/1‑143 (MQ=255) tcAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTccc > 2:290132/1‑143 (MQ=255) tcAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAAAAAGAATCATTGTTATAACAATTACTAATAAGTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCAGAAACGGATGCCAATCACAAGACCGCTAccc > 1:25611/1‑143 (MQ=255) aacaaATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAAGTAGAATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGAATTCCAATCACAAGACCGATCCCCCg > 2:194565/1‑143 (MQ=255) ttgctCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGc < 2:48014/143‑1 (MQ=255) ccATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTccagcca > 1:7492/1‑143 (MQ=255) ttGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACGCTGt > 1:45214/1‑143 (MQ=255) gATAATAAGAATCATTGTTATAAACATTGTTCGCAAGCAGTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGCATTTCCAGCCAGACACCGtga > 2:217595/1‑141 (MQ=255) aaGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCGATGCGGCGAATTGCCAGCCAGAGACTGGTAGGTGCg > 2:75145/1‑143 (MQ=255) aaGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAAATCATCAGTGAGACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCg > 1:68469/1‑143 (MQ=255) aTCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATg < 1:414586/143‑1 (MQ=255) ttATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTCTTAGCTCCGGATGGCAAACt > 2:284402/1‑143 (MQ=255) ttttATGAGTTATACGGAGAGCAGAAAACTCACGCCTTAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACGCGCTACGGTCCGGAAGGCGAACGGATCATCGGTCGTGACGGGc > 1:388921/1‑143 (MQ=255) | GAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGGC > NC_000913/1789480‑1789751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |