| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,940,584 | C→A | 100% | R351L (CGT→CTT) | mepM ← | peptidoglycan DD‑endopeptidase MepM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,940,584 | 0 | C | A | 100.0% | 40.0 / ‑6.7 | 17 | R351L (CGT→CTT) | mepM | peptidoglycan DD‑endopeptidase MepM |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (11/5); total (12/5) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGA > NC_000913/1940448‑1940721 | aTAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCGAGTGCATATAACGCGTGGTGTAGCTGCGACCGTGGAGAATAg > 1:144464/1‑143 (MQ=255) gttagtgCCCGGTTGAACGTCTTGTAGTACCGTAAAGCGGGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGTTGCGACCATGAAGAATAGCCACataa < 2:366464/137‑1 (MQ=255) gATGCGGGTGGGTTGGACGTCCGGTAGTACCGGAGAGCGCGGTACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCAGATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACataa < 2:107569/143‑1 (MQ=255) gTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCAACAGAATCTTGCGCAAGTGCATATGAAGGGGTGTGGACCGACCACCATGAAGAATAGCCACGTAATAACCtgctgc > 1:352841/1‑143 (MQ=255) ccGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCGGCTGCGCCACTGCg > 1:343450/1‑143 (MQ=255) aGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACACAATAACCTGCTGCGCCACTGCGTTGGGCAACcaccac > 2:460364/1‑143 (MQ=255) tACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCg > 2:433374/1‑143 (MQ=255) ccACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATACCGCGTGGTGTCGCTGCGACCCTGAAGAATCACCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCGCCGAc > 2:183717/1‑143 (MQ=255) ccTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACt > 1:434166/1‑143 (MQ=255) cccGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTAcc < 2:359003/143‑1 (MQ=255) cccGGTTCCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTAcc < 1:24944/143‑1 (MQ=255) ggTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCTCCTGCGTTTGGCACCCACCACTTCACCGTCACCCCCTGCCAGCACTGGCCTACCCtc > 1:451730/1‑142 (MQ=255) ccgcAAGTGCATATAACGCGTGGTGTAGCTGCGACCATAAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCaa < 1:267053/142‑1 (MQ=255) aaGTGCATATAACGCGTGGTGTAGCTGCGACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACGCACTGAAAGCGCTGGCGTACCCTGCGGCATGGCGAAATCAACAcc > 1:42446/1‑143 (MQ=255) gTGCATATAACGCGTGGTGTAGCTGCGACCATGCAGAATAGCGACATAATAACCTGCTGCGCGACTGCGTTTGGCAACCACCACTTCACCGTCCCCCACGGACAGCACTGGCGTACCCTGCGGCGTGGCGAAATAAACCCctc > 1:42249/1‑143 (MQ=255) gACCATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCAGTAAca > 1:279507/1‑142 (MQ=255) cATGAAGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCCACGCGCCCGGTCACCGGa > 2:46456/1‑143 (MQ=255) | ATAGTGCAGATGCGGCCCGGTTGAACGTCCGGTATTACCGGAAAGCGCGATACGGTCGCCACGTTTCACCTTCTGTCCCGGTTTCACCAGAATCTTGCGCAAGTGCATATAACGCGTGGTGTAGCTGCGACCATGACGAATAGCCACATAATAACCTGCTGCGCCACTGCGTTTGGCAACCACCACTTCACCGTCACCCACTGAAAGCACTGGCGTACCCTGCGGCATGGCGAAATCAACACCTCTGTGTGGTGCAACGCGACCGGTCACCGGA > NC_000913/1940448‑1940721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |