| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,941,326 | C→T | 100% | D104N (GAT→AAT) | mepM ← | peptidoglycan DD‑endopeptidase MepM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,941,326 | 0 | C | T | 100.0% | 53.7 / NA | 20 | D104N (GAT→AAT) | mepM | peptidoglycan DD‑endopeptidase MepM |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/7); total (13/7) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGC > NC_000913/1941188‑1941440 | gCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATAGAGAATGCTGCTTAGCGTATTGCCa > 2:44708/1‑143 (MQ=255) gTTCGCCGTCCGCGGTTAATGTACAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACACATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCCGTg > 2:175319/1‑143 (MQ=255) ccGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCAATATCAATAACAAATGGAGTGAGAATGCTGCTTAGCGTATTGCCAGTGGAaac > 1:483471/1‑143 (MQ=255) cgcgGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGGGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCGTAGCGTATTGGCAGTGGAAAGAACat > 2:402893/1‑143 (MQ=255) cgGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCCTATCAATACCGTACTGATTGAGAATGCGGCTTAGCGTATTGCCAGTGGAAACTACatag > 2:26602/1‑142 (MQ=255) aTGTCCAGGCGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGTATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGc < 2:17821/143‑1 (MQ=255) gTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACGATACTGATCGAGAGTGCTGCTTAGCGTATTGCCAGTGGAAACACCATATTCATGCACGCCCGCTTCACCGGCGACTTTGTCATCCa > 2:131770/1‑143 (MQ=255) cAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTTAGAAGGCTGCGTAGCGTATTGCCAGTGGCAACAACATATTCATGCACGCCCGCTTCACCTGCGCCTTTGTAATCGAGTTctt > 2:109354/1‑141 (MQ=255) cTTCGTCGGCCGCAGCCAGTTGGGTGATAACAATCATATCTGTAGCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTgg < 1:329841/143‑1 (MQ=255) ggCCGCAGCCAGTTGGGTGATATCACCTATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCGCGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATgg < 2:41711/143‑1 (MQ=255) cAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTCGTCATCCAGTTCGTCCTGGGGAATGGCTTcc > 2:463012/1‑142 (MQ=255) aGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCTCGTTCGTCCGGGGGAATGGCTCCAt > 2:484885/1‑143 (MQ=255) gTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACCCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGc > 1:244186/1‑143 (MQ=255) tGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCa < 1:309429/143‑1 (MQ=255) tCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTTTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGTAGCTTg < 1:126809/143‑1 (MQ=255) ccATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCa > 2:413900/1‑143 (MQ=255) tCAATACCATACGGATTGAGAATGCTGCTGAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCgg < 1:154857/143‑1 (MQ=255) ccATACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAACCAACAGATTCATGCACGCCCGCTGCACCGGCGATTTTGTCATCCAGTTCAGCCTGGGGAATGGCTGCATCTTCTTGTGCAGCTTAATCAAGCGGCTCACt > 2:271432/1‑143 (MQ=255) aTACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCACTTTACCGGCGAGTTTGTCATACAGTTCGTCCTGGGGACTGGCTTCATCTTCTTGTGTAGCTTGATACACCGGCTGAATgg > 2:7153/1‑143 (MQ=255) tACTGATTGAGAATGCTGCTTAGCGTATTGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGc < 2:286883/143‑1 (MQ=255) | GCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCGATTTTGTCATCCAGTTCGTCCTGGGGAATGGCTTCATCTTCTTGTGCAGCTTGATCAATCGGCTCACTGGC > NC_000913/1941188‑1941440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |