Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,132,787	A→C	100%	I204S (ATC→AGC)	wcaA ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	31.0 / NA	14	I204S (ATC→AGC)	wcaA	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/9);  total (5/9)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT  >  NC_000913/2132652‑2132922                                                                                                                                        |                                                                                                                                        gggTAGAGGGGAAACAGCTGCTATTTTTTGCCGGCGCGGTCGAATTTGTCTTCGTGCGTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTGTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt                                                                                                                                  <  1:350183/142‑1 (MQ=255)            aaaCAGCGGATATTTGTTGCTGGCGCGGTAAAAATTAGGATTTTTTTTTCTGTAAAAGTGGGAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                       <  2:212251/143‑1 (MQ=255)               cAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGCCtcttctccct                                                                                                                    >  2:433484/1‑139 (MQ=255)                        ttttgggCTGGCGCGGGCGGATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                           <  1:420676/137‑1 (MQ=255)                         tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGGTCTGCATTTCACTGTGATTGATATGAAGGCTCTGCGTCGACTCTTCTACTTTCCACGGTTc                                                                                                          >  2:72197/1‑143 (MQ=255)                               cTGGCGCGGTCGAATTTGTCTTTGTGCTGGCGGTAAAAGTGGAAGCACCCGGAGAACACCTTCAGTTACGTGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                    <  1:355949/143‑1 (MQ=255)                                                            gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTGTCCTTTCCCCGGTTCGCCGTACTCCACCACCATCCGCAGGAAAATGTCGt                                                                       >  1:370433/1‑143 (MQ=255)                                                                                   agaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTTCTTTCCACGGTGCGCCGTAGTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                <  1:162571/143‑1 (MQ=255)                                                                                       tgtcttCGGGGCCGAGGTGGTCTGGATTCCGCCGTGATTGACATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                            <  2:206771/139‑1 (MQ=255)                                                                                                        cgATCTGCCTTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCTACGGTTCGCCGTACTCTACCACCATCCGCAGGAAGATGTCGTAATCACGTGCCGGTTTTAGTTCGGTATCGAACAAACACTCTTTg                           <  2:81916/142‑1 (MQ=255)                                                                                                          aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCCGGCCCCTATCGGACACACActctgcgca                         >  1:472524/1‑138 (MQ=255)                                                                                                                     aCCGTGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc              <  1:184504/143‑1 (MQ=255)                                                                                                                               atatGCAGGCTCTGCGTCGCCGCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc    <  1:33024/143‑1 (MQ=255)                                                                                                                                 atGCAGGCTCTGCGTCGCCTCTTCTACTTTCTAAGCCGAACAGTAGGCAACCACCATCCGCAGGAAGATGTTGTGATCCTGTTCCGCGTGCAGTTCGGTGTCGAATAACTACTCTTTTCAAAGCCATGCCCAGGTCAAgcgct  >  2:426068/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT  >  NC_000913/2132652‑2132922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑