| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,661,872 | A→G | 82.8% | V87A (GTA→GCA) | iscR ← | DNA‑binding transcriptional dual regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,661,872 | 0 | A | G | 82.8% | 54.9 / 7.9 | 29 | V87A (GTA→GCA) | iscR | DNA‑binding transcriptional dual regulator IscR |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base G (11/13); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.26e-01 | |||||||||||
TTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAT > NC_000913/2661730‑2662010 | ttCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCACCCGCCTGTACCCTGACAACGGGCGGCACCTa > 1:323879/1‑143 (MQ=255) aaCCAGTTCGCCTAAATTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAACTCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCaa < 1:283285/143‑1 (MQ=255) cAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTTAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCATGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACgg > 1:435932/1‑143 (MQ=255) gCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGCCATCTGCAGATTCGTCAACGGCGCtcg > 1:297980/1‑141 (MQ=255) aaGGAATGTTGTTGAGAAAACCGGTGCGAAGGCCACTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACt < 1:329484/143‑1 (MQ=255) aGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACtt > 1:22359/1‑143 (MQ=255) tgttgtTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCa > 1:225705/1‑143 (MQ=255) gaAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCTGGCATTTATCGCCGCCCTGGCAGCAGCCTTTACCCTGAGAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTACTCACTTCGCGAAAGGCGAtg > 1:121416/1‑143 (MQ=255) ccGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCTGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATgctgct < 2:392613/143‑1 (MQ=255) cGGTGAGACGGTCGCTCAAAGCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGctgctg < 2:207207/143‑1 (MQ=255) tcagccGGTCGCTCGAACCACGCCACAGCCCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGCGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCa < 2:159251/138‑1 (MQ=255) ggTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTg > 1:425119/1‑143 (MQ=255) gTCGCTCAAATCACGCCGCAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 2:89536/143‑1 (MQ=255) ctCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGc < 1:359640/142‑1 (MQ=255) cAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACa < 1:113484/143‑1 (MQ=255) tCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTGCGCCAACGGCGATGCTGCTGGCATCTTCGCCTAACacaga > 2:175988/1‑139 (MQ=255) caGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGc < 1:100310/143‑1 (MQ=255) cgcgTAGGTCTGGCAGTTATCGCCTCCCTGGCAGCCGCCGTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCGAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTg < 2:404129/143‑1 (MQ=255) ggCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATCACCACCGCCTGGTCCACGTAcc > 2:203182/1‑142 (MQ=255) aTTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATCACCACCGCCTGGTCCCCGTACGCTg > 1:337839/1‑143 (MQ=255) tCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATGACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGCCCACGGCCGCTGGCAAc > 1:120957/1‑143 (MQ=255) tACCCTGGCAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACgg < 2:297980/143‑1 (MQ=255) ccTGACAACGGGTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTGTTACGCAGACGGGaa > 1:8722/1‑143 (MQ=255) ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGCTAACCACCGCCTGGTCCCCGTACGCTGGACACCCGACCCATTTTACGCAGACGGGag > 2:398108/1‑142 (MQ=255) ccTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCCTTTTTACGCAGACGGGaa > 2:392560/1‑143 (MQ=255) aCGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAgtt < 1:132870/143‑1 (MQ=255) ggTGGCATCTGCAGATCCGTCAACTGCGCTGATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAgttgtt < 2:425119/143‑1 (MQ=255) gTGGCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTc > 1:414516/1‑143 (MQ=255) ggCATCTGCAGATTCGTCAACGGCGCTAATTACTTCGCCTACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCa < 2:78862/143‑1 (MQ=255) aTCTGCAGATTCGGCAACGTCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAt < 2:130262/143‑1 (MQ=255) | TTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCTGGCAGCCGCCTTTACCCTGACAACGGGTGGCATCTACAGATTCGTCAACGGCGCTAATTACTTCGCCAACGGCGATGCTGCTGGCATCTTTGCCTAACAGATAACCACCGCCTGGTCCACGTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGAT > NC_000913/2661730‑2662010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |