BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,398	T→G	100%	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	54.7 / NA	23	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (5/18); total (5/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTG  >  NC_000913/3041264‑3041521

ctTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccgcca                                                                                                                     >  1:375171/1‑143 (MQ=255)

ctTTTACCATTAGTCATTAATACGCCCTTTAGCAACTCAGCACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTCCCGCTGACTCTAATGACGCTTGATGACTGGGCGCGTGccacca                                                                                                                     <  2:171234/143‑1 (MQ=255)

    tcccATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTAc                                                                                                                 <  1:207265/141‑1 (MQ=255)

          tAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcgc                                                                                                           <  1:447299/143‑1 (MQ=255)

             tCATTAAGTCGCCGTTAAGCTACTCAGGACTCTATTATGGCTTTTACACCTGTTCCTCCCCGTCAGTCTACGGCTTCTGCCCGTTTACCGCGGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa                                                                                                        <  2:39405/143‑1 (MQ=255)

                      cGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaaaa                                                                                               <  1:104899/143‑1 (MQ=255)

                          gTTAAGCCACTCGGGACTCTATTAGGGATTTTACCCCTTTTCCTCTCCATACGCCGTAGGAATCTGGGCGTTTGATGCTGATAAAAATGACGCTTGCTGTCTGCACGCGTGCCACCATTACTGGCGCGGACAGCGAAAAatat                                                                                           <  2:294109/143‑1 (MQ=255)

                                    tCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAGATATATGTAGGGTc                                                                                 >  2:280311/1‑143 (MQ=255)

                                          atatattatGGCTTTTACACCTGTCCCCCCCCGTCAGCCTACGGCTCCCGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGa                                                                           <  1:29885/140‑1 (MQ=255)

                                             tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGACCGTTTACCGCTGACGCTGATGGCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGACAggggtagg                                                                        >  2:62224/1‑137 (MQ=255)

                                                 atGGCTTTTACCCCTTTTCCTCCCCGTAATCCTACTGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGa                                                                    <  2:211384/143‑1 (MQ=255)

                                                  tGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGAt                                                                   <  1:388448/143‑1 (MQ=255)

                                                      ttttACACCTTTTCCTCCCCTTCAGCCTACGGCTTCTGCCCGTGTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa                                                               <  1:140105/143‑1 (MQ=255)

                                                               tttccatccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGCAAAATATATGCAATGTCAGGTGAAAGCTGATGTCAGCCAGATgg                                                      <  2:453834/137‑1 (MQ=255)

                                                               tcgtcctccCCTTCAGCCTACGGCTTCTCCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTCCCACCATTACTCGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATgg                                                      <  2:424979/140‑1 (MQ=255)

                                                                    ctccCCGTCAGCCTACGGCTTCTGCCCGTTTACTGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaa                                                 >  1:488626/1‑143 (MQ=255)

                                                                         cgccAGCCTACGGGCTCTGCCCGTTGACCGCTGACGCTGATGACGCTTGATGACTGGGAGAGAGCAATCATTACTGGCGCGGACAGCGAAAAATATATCAAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCa                                            <  2:453390/140‑1 (MQ=255)

                                                                              gCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCAcc                                       <  1:441772/143‑1 (MQ=255)

                                                                                      cTTATGACCGTGAACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgcc                               <  1:199710/143‑1 (MQ=255)

                                                                                              cGTTTACCGCTGACGCTGATGACGCATAATGACTGGGCGCGTGCCGCCATTACTGGCGCGGACCGCGAACAGTATATGCAGGGTCAGGGGACAGCAGATGTCAGCCAGCTGGCAGAAGAACACCGCCCGATCGCCGcccgcgg                       >  2:195316/1‑139 (MQ=255)

                                                                                                         gacgcggaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCTGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAg            <  1:165954/143‑1 (MQ=255)

                                                                                                                  ggcGCTGGATGCCTGGGCGCGTGCCACCATGACTGGCGCGGACAGCGAAAAATACATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtgt   <  2:91200/141‑1 (MQ=255)

                                                                                                                   aCGCGTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGAGAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCATGTGATAGTCAGCCATTGGGACGCCAATGGTAAAAtgtg  <  2:104716/143‑1 (MQ=255)

CTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTG  >  NC_000913/3041264‑3041521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`