| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 710,489 | G→C | 100% | A53G (GCT→GGT) | fur ← | DNA‑binding transcriptional dual regulator Fur |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 710,489 | 0 | G | C | 100.0% | 80.4 / ‑6.7 | 30 | A53G (GCT→GGT) | fur | DNA‑binding transcriptional dual regulator Fur |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (11/18); minor base A (1/0); total (12/18) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAG > NC_000913/710358‑710625 | cGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATAACAGCGTCGTCAAACTGGTTCAGTACGAGATATACCGAACACACACCAAta > 2:418996/1‑142 (MQ=255) aGTCGAGGCAGATCAGGTGATCGTGGTGCTGTTGCTGTGTCCGTTCACCTACGGCTTTACCGCCTTCAAAATTGTGGCGGATGACGATACCAGCGTCGTAAAACTAGGTCAGTACGCGATATACCGTACCCAGACCAATttct > 1:122421/1‑143 (MQ=255) aGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAACCCGCCCAATTTCTTCAcc > 2:399026/1‑143 (MQ=255) aGGTGATCTGGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAt < 2:43047/143‑1 (MQ=255) aGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGTTTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGAt < 1:446352/143‑1 (MQ=255) tgATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACGGTACCCAGACCAATCTCTTCACCCATATCGATCAGACGTTTGtc > 1:42624/1‑142 (MQ=255) tGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATaa < 1:295252/143‑1 (MQ=255) tGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATaa < 1:342221/143‑1 (MQ=255) ttGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAAt < 1:344903/143‑1 (MQ=255) tgtCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAGATCTTCCg > 1:123503/1‑143 (MQ=255) tCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCa < 1:4267/143‑1 (MQ=255) tCAGTTCAAATACGGATTTACCGCCTTCAAAAGTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCa < 2:482812/143‑1 (MQ=255) gTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTg < 2:487537/143‑1 (MQ=255) tCAAACACGGATCTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGAc < 1:22388/143‑1 (MQ=255) aaTACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCACATCGATCAGACGTCTGTATAAAACTTCCGCACtaccgag > 2:298975/1‑137 (MQ=255) tACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTACTTCACCCATATCGATCAGGCGGTTGTATAAATCTTCCGCGCTGACGTGag > 1:54327/1‑142 (MQ=255) aCGGATTTACCGCCTTCAAAAGTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATg < 1:313952/143‑1 (MQ=255) tcgtcTTCAAGCTTGTGTCTGGTGAGGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGATCAATTTCTTCACCCTTATCTATCTGACGTTTGTATAAATCTTCCTCACTGACGTGATGGTTGTCCgg < 2:192270/139‑1 (MQ=255) cTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTGAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGCTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTcc > 1:313794/1‑143 (MQ=255) aaaaTTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTgaa > 1:192916/1‑143 (MQ=255) aTTGTGGCGGGTGACGATACCAGCGGCGTCAAACTGGTTCAGTACGCGATATCCCGTTCCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTgaagaa < 2:290970/143‑1 (MQ=255) tgGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTc < 1:42892/143‑1 (MQ=255) gCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTATTCACCCATATCGATCAGACGTTTGTATAAATCTTCAGCACTGACGTGATGGTTGTCCGGCTCATGAAGAACTTCCa > 2:149137/1‑143 (MQ=255) cGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGAttttt < 1:418996/143‑1 (MQ=255) cgtcAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGc < 1:295666/143‑1 (MQ=255) tcAAACTGGTTCAGTACGCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGAGGTTTGTATAAATCTTCCGCACTCCCGTGATGTGTGTCCGGCGTATCAACGACAGCCAGGATTTGTAAACGAGGacccgt > 2:78061/1‑138 (MQ=255) accTGGTTCAGTACTCGATATACCGTACCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTAc < 2:269195/141‑1 (MQ=255) aCGCGTTATACCGTACCCAGACCAATTTCTTCACCCATCTCGATCAGACGTTTGTATACATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGc < 2:355597/143‑1 (MQ=255) gcgATATACCGTACCCAGGCCAATTTCTTCACCCATATCGATCAGACGTTTGTATACATCTTCCGCACTTACGTGATGGTTGGCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGctt < 2:365863/143‑1 (MQ=255) aCCGTACGCAGACCAATTTCTTCACGCATATCGATCAGACGTTTGTATACATCTTCCGCACTGACGTGATGGGTGTCCGGCTCCTGTAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCGGCTTTCTCTAg > 2:369171/1‑143 (MQ=255) | CGCAGTCGAGGCAGATCAGGTGATCGTGGTGATGTTGCTGTGTCAGTTCAAATACGGATTTACCGCCTTCAAAATTGTGGCGGGTGACGATACCAGCGTCGTCAAACTGGTTCAGTACGCGATATACCGTAGCCAGACCAATTTCTTCACCCATATCGATCAGACGTTTGTATAAATCTTCCGCACTGACGTGATGGTTGTCCGGCTCCTGAAGAACTTCCAGGATTTTTAAACGAGGAAGCGTTACTTTCAGGCCAGCTTTCTTTAG > NC_000913/710358‑710625 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |