| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,026,927 | A→T | 100% | V30E (GTG→GAG) | mgsA ← | methylglyoxal synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,026,927 | 0 | A | T | 100.0% | 48.4 / ‑6.7 | 17 | V30E (GTG→GAG) | mgsA | methylglyoxal synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (10/6); minor base C (1/0); total (11/6) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTT > NC_000913/1026789‑1027038 | gcgcCAACCTGCTGGTCCCCCCCCATTGCGCCACTCAACATCGCGTTGACGTTCACGCCGGTCGCGCGGGACATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCa < 2:354106/143‑1 (MQ=255) aaCCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGGTGTTCCAGCAACGGCTGCTGCCTTCCCCCCCACCtca > 2:458621/1‑143 (MQ=255) ctgctgGTCGCCCCCCATTGTGCCACTCAACATCGCGTTGACGTTCATGTCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCtcatca < 1:273228/143‑1 (MQ=255) aCCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCAtttgtt > 2:310147/1‑143 (MQ=255) cACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCgtgt < 1:197770/143‑1 (MQ=255) cAACCTCGCGTCGGCGTCCATGCCGGTCGCGCGGCAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGcca < 1:8933/143‑1 (MQ=255) tGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCcgc > 1:395752/1‑143 (MQ=255) ggTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAg < 1:144766/143‑1 (MQ=255) gcgGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCCCCAGCGCAATATGTTTCCGCGCAGGTAAAg > 2:454351/1‑143 (MQ=255) ttACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAACATGTTTCCGCGCAGGTAAAGTGCGCGTCGTCAg > 2:450604/1‑143 (MQ=255) aCCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTACAATGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGtt > 1:56308/1‑143 (MQ=255) ccGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTc > 1:230800/1‑143 (MQ=255) ttcctcgtGCATACAGCACGTGTGGTTCCAGTAACGGTTGATGCCGTCCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATg < 1:297754/136‑1 (MQ=255) ttGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGCTAAAGTGCGAGTCGTCAGTTCCATACTGTACacc > 2:15976/1‑141 (MQ=255) aCAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCGGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATACGTAGt > 1:268476/1‑143 (MQ=255) gTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTACGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTaa > 2:268537/1‑143 (MQ=255) gtgtTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACttt > 2:60025/1‑143 (MQ=255) | GCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTT > NC_000913/1026789‑1027038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |