Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,196,220	C→T	100%	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	100.0%	27.7 / NA	13	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACG‑GTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGA  >  NC_000913/1196092‑1196297                                                                                                                                  |                                                                              ccTGGTGCAAACATCGGTGAAGAATGCGCCCAGTTTGCA‑CCAGACGGGTTTACTGCGCCGAAATATGTCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGa                                                                 <  1:352905/143‑1 (MQ=255)  cTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGCAGCCACCCACG‑GTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGaa                                                                >  2:131518/1‑143 (MQ=255)           aCATCGGTGACGGATGCGCCCTGTTTGACGCCACCCACG‑GTACTGCGCCGACATATGCCGGCCAGGACAGAGTAAATCCTAGCTCTATTATTCTCTCCGCTGAGATGATGCTGCTCCTTATGGTTTGGACTGAAGCGGCTGAc                                                       <  2:46180/143‑1 (MQ=255)                 gTGACGAATGCGCCCTGTTTGCAGCCACCCACG‑GTACTGCGCCGAAGTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCGCCGCTGAGATGATGCTGCGCCATACGGGTTGGACTGAATCGGCTGAcctgata                                                 >  2:39692/1‑137 (MQ=255)                 gTGACGAATGCGCCCTGTTTGAAGCCACCCACG‑GTACTGCGCCGAAATATGCCGGTCAGTACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGAtt                                                 >  1:216269/1‑143 (MQ=38)                       aaTGCGCCCTGTTTTAAGCTACCTACG‑GTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa                                           <  1:148524/143‑1 (MQ=37)                       aaTGCGCCCTGTTTGAAGCCACCCACG‑GTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGACGCGGCTGACCTGATTGTTacc                                           <  1:308162/143‑3 (MQ=38)                            gcgttGTTTGAAGGCACCCACG‑GTACTGCGCCGAAATATGCCGGTCAGGACAAATTAAATCCTGGCTCTAGTATTCTCTCTGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                      <  2:382417/139‑1 (MQ=25)                            gcCCTGTTTGAAGCGTCGAAAG‑GTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCGGGCTCTATTATTCTCTCCGGTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                      <  2:403849/143‑1 (MQ=25)                                    tGAAGCCACCCACG‑GTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGATCTATTATTCTCTCCGCTCAGATGATGCTGAGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                              >  2:262703/1‑143 (MQ=25)                                    tGAAGCCACCCACG‑GTACTGCACCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGCCCTGATTGTTAAAGGTATGGAAGgcg                              >  1:78291/1‑143 (MQ=37)                                                               aaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt    <  2:351444/143‑1 (MQ=17)                                                                 tatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa  >  1:138063/1‑143 (MQ=17)                                                                                                                                  |                                                                              CCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACG‑GTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGA  >  NC_000913/1196092‑1196297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑