| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,085 | Δ1 bp | coding (2069/2664 nt) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | . | 98.2% | 238.0 / ‑0.0 | 59 | coding (2069/2664 nt) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (28/28); minor base A (0/1); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
GTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124947‑125221 | gTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTa > 1:925246/1‑143 (MQ=255) tCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTAc > 2:1034474/1‑143 (MQ=255) cacaGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACg < 2:40511/143‑1 (MQ=255) caTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGCAGTTGCTGTCATCATGCATGACGGTCTGAAGCGTATGTACGGTGACAGACAAGAGAACGATTACAACTAC‑CCACTACGCTGAACGa > 2:502557/1‑143 (MQ=255) aTTCAGTCGATGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGaa < 1:12645/143‑1 (MQ=255) gCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACca > 1:734467/1‑143 (MQ=255) gCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACca < 1:1077810/143‑1 (MQ=255) aCTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGGACGAAAACTACcaacag > 2:493907/1‑139 (MQ=255) cgcGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGc < 2:649544/141‑1 (MQ=255) cccGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGc > 2:137924/1‑143 (MQ=255) ctctATCTCTTTCGATCCGGCTTACGCTTACGAAGTTGCTGTCATTTTTCATGACGGTCTGGAGCGTATGTACGGGGAAAAACAGGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGcc < 1:354479/140‑1 (MQ=255) cTGTAGCTGTTACGAGCGGGCTGACGCGGACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGcc < 1:191568/143‑1 (MQ=255) tGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCg < 1:687376/143‑1 (MQ=255) tGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGACGGCAATGCCg > 2:71998/1‑143 (MQ=255) gTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTAGGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAACACTACCCCGTGCCCGCAACGCggg > 1:21093/1‑143 (MQ=255) tATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGa > 1:377974/1‑143 (MQ=255) tctcTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTAGGTACGGTGAAAAAAAAGAGAACGTTTACTACTACA‑CACTACGGTGAACGAAAACTACCACATGCCGGCAATGCCGGGAg > 1:252910/1‑143 (MQ=255) tctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGAATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGTGCGCCACCTCCCTCCTGCCGCCAGTGCCGGAATGt > 1:80336/1‑143 (MQ=255) ctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCATTGCCggcatgtg < 2:925246/143‑7 (MQ=255) ctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAGTGCCGGACgggg > 2:835791/1‑141 (MQ=255) aCGACCCGGCTTACGCATACGAAGTTGCTGTCATCATGCAGGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTg < 2:1119136/143‑1 (MQ=255) ggcccGGCTTACGCGTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAg < 2:661632/141‑1 (MQ=255) cGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATCCCGGCAATGCCGGGAGGTGCTGAGGAAg > 2:714438/1‑143 (MQ=255) cGGCTTACGCTTACGAAGTTGCTGTCATCAGGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAg < 2:358109/143‑1 (MQ=255) gcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGACGAGGAAGGTacgcgt > 2:682996/1‑138 (MQ=255) cttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGACGGTGCTGAGGAAGGTATCCGTa > 2:718842/1‑143 (MQ=255) ttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTaa < 1:137924/143‑1 (MQ=255) tacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTaaa < 1:866455/143‑1 (MQ=255) cTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGATTGAGAAGAGTACGTTTACTACTACA‑CACTAGGCTGAACGAAAACTACCAAATGCAGGCAATGCCGGAAGGTGCTGAGTAAGGGAACCGTAGAGGTATCGACa > 2:80237/1‑143 (MQ=255) cTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACa > 1:913213/1‑143 (MQ=255) cTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGCAACCGAATAGGTGTCTaga > 1:151117/1‑141 (MQ=255) cTGTCAGCATGCATGGCGGTGTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACGACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACa < 2:587539/143‑1 (MQ=255) gTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑AACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGCGCTGAGGAAGGGAACCGTAAGGGCATCTACaaa > 1:956028/1‑143 (MQ=255) tcatcaTGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAAc > 1:165850/1‑143 (MQ=255) agcatgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTc < 1:1037438/141‑1 (MQ=255) catgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGa < 1:409694/143‑1 (MQ=255) tgcatgACGGTCTGGAGCGTATGTACGGTCAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa < 1:664509/143‑1 (MQ=255) tgcatgACGGTCTGGAGCGTATGTACGGGGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCCCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa < 1:57872/143‑1 (MQ=255) gcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGATGAGGAAGGTATCCGTAAAGGTATCTACAAACACAAAAc > 2:106520/1‑143 (MQ=255) aCGGTCTGGAGCGTATGTACGGGAAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATACCGGCAATACCGGAAGGAGCTAAAGAACGTATCCGAAAAGGGATCTACAGACTCGAAACTATTg > 2:881973/1‑143 (MQ=255) gTCTGGGGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAg < 1:1075494/143‑1 (MQ=255) tGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTa < 2:734467/143‑1 (MQ=255) aGCATATGTACGGTGAAAAACAAGAGAACGTTTACTACTAC‑GCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACGATGGAAGGTAGCa > 2:1002459/1‑143 (MQ=255) gCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCaa < 1:1108810/143‑1 (MQ=255) gTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAg < 2:887630/143‑1 (MQ=255) taaaaaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTTAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCa < 2:882543/141‑1 (MQ=255) tGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCa < 1:1034474/143‑1 (MQ=255) tGAAAAACAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATGGAAGCTATCATGGGTCAAGCTCa > 1:472067/1‑143 (MQ=255) aaaaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTTAgc > 2:267589/1‑143 (MQ=255) aaaCAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTAGCTACAAACTCGAAACTATCGCAGGTAACAAAGTGAAAGTTCCgcac > 2:322106/1‑141 (MQ=255) cAAGAGAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGCAGGTAGCAAAGGTAAAGTTCAGCTGCTc > 1:320826/1‑143 (MQ=255) aaGAGCACGTCTACTAC‑ACCACACTACGCGGCACGAAAACTACCCCACGCCGGCAGTGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAATCTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCg < 1:775028/143‑1 (MQ=255) agagAACGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCgg < 1:220785/143‑1 (MQ=255) tcacttttACTACTCCA‑CGCCACACTTAACGATAACTACCACATCCCGGCAATGCCTGAATGTGCTGAGGAAGGTCTCTGTAAAGGTATCTACAAACTCGACCCGATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTc < 2:917908/138‑1 (MQ=255) gaACGGTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTc < 1:785998/143‑1 (MQ=255) aaCGTTTACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTcc > 1:472363/1‑143 (MQ=255) ttACTACTACA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGAAAGGTGCTGAGGAGGGTATCCGTAACGGTATCTACAACCTCGAAGCGATTGAAGGGAGCAAAGGTAGAATTGAGCTGCTGGGCTCCGGttg > 2:548417/1‑142 (MQ=255) actactacA‑CACTACGCTGAACGAAACCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTa < 1:791681/143‑1 (MQ=255) tactacA‑CACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTGCAGCTGCTCGGCTCCGGTGCTATc > 2:851710/1‑143 (MQ=255) | GTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124947‑125221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |