Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,789,616	C→A	R2S (CGT→AGT)	ydiE →	PF10636 family protein YdiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,789,616	0	C	A	100.0%	198.2 / NA	63	R2S (CGT→AGT)	ydiE	PF10636 family protein YdiE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/31);  total (32/31)

TCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGG  >  NC_000913/1789477‑1789750                                                                                                                                            |                                                                                                                                       tCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGtt                                                                                                                                     >  1:125609/1‑143 (MQ=255)    gAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCCATTATTATTAATTTTTATGAGTtcta                                                                                                                                  >  1:570221/1‑143 (MQ=255)     aaTGCGGGCCGATTCCTGACTGAATGGGCATGTCTGCGCAACTTGTTGTCGTCTCAACAAATTACTGTTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATAc                                                                                                                                 <  1:562389/143‑1 (MQ=255)      aggcttGCCCATTCCCGATGTATTGCGCATTTCTGCGTAACTTGTTGTCTTCTCAACAAATTACTGCTTGTTCTGGTCAGTCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACg                                                                                                                                <  2:911899/138‑1 (MQ=255)        gCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGa                                                                                                                              >  2:1034977/1‑143 (MQ=255)           tGCCCATTCCGTCAGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATCTTGATAATAAGAATCCTTGCTATATCACTTCTTATTATTTCTTATGAGTTATACCGCTAg                                                                                                                           >  1:380610/1‑143 (MQ=255)            gCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGc                                                                                                                          <  2:388410/143‑1 (MQ=255)            gCCCATTCCGTCAGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGc                                                                                                                          >  1:553322/1‑143 (MQ=255)              ccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTTCTCTGGTCCGCAATAATATTGATAATAAGAATCATTGTTATATCAATAATTATTCATTTTTATGAGTTATACGGATAGCAg                                                                                                                        >  2:450655/1‑143 (MQ=255)              ccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAg                                                                                                                        >  2:1016326/1‑143 (MQ=255)              ccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAg                                                                                                                        >  1:41856/1‑143 (MQ=255)              ccATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTCTTATGAGTTATACGGATAGCAg                                                                                                                        >  2:144217/1‑143 (MQ=255)                  tCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGaaaa                                                                                                                    <  2:491213/143‑1 (MQ=255)                     tGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCCATTGTTATCTGCAAAGATGAGTGATACGGATAGCAGAAAACTc                                                                                                                 <  2:353104/143‑1 (MQ=255)                     tGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTAATGCTTCCTCTGGGTAGCCATAGTGTTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTc                                                                                                                 >  1:264777/1‑143 (MQ=255)                         ggaagtggCATTTCTGCGCAACTTGTTGTCTTCGCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATGGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGc                                                                                                             <  2:388005/137‑1 (MQ=255)                         ggAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATTGCAGAAATCTCCCGc                                                                                                             >  1:1125638/1‑143 (MQ=255)                           aaTGGGCATTTCTGCGCAACTTTTTGTCTTTTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAATAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCt                                                                                                           <  2:1137198/143‑1 (MQ=255)                              gggCATTCCTTGGCAACTTGTTGTCTTCTCAACAAATGACTGCTTGCGCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGaa                                                                                                        <  2:869473/143‑1 (MQ=255)                                gCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAAc                                                                                                      >  1:35053/1‑143 (MQ=255)                                gCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAAc                                                                                                      >  1:666973/1‑143 (MQ=255)                                   tttCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGa                                                                                                   <  2:963427/143‑1 (MQ=255)                                    tgcTGCGCAACTTGTTTGCTTCTCAACAAATTACTGCTTGCTCTGGTCAGACATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGAt                                                                                                  <  2:590459/141‑1 (MQ=255)                                     tCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATCCGGATAGCAGAAAACTGCCGCCTGAAACGGGTg                                                                                                 >  1:415875/1‑143 (MQ=255)                                      cTGCGGCACTTGTTGTCTTCTCAACAAATTACTGCTTGACCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGc                                                                                                <  1:1096331/143‑1 (MQ=255)                                      cTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGc                                                                                                <  1:351039/143‑1 (MQ=255)                                                 gttgtCTTCTGTGCAAATTACTGCTTGCTCTGGTCAGCCATATTATTGAGAATGAGATTCATTGTTATAGTAAGTATTATGAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTTAAACGGATGCCAATCACAAGa                                                                                     <  2:688689/143‑1 (MQ=255)                                                  ttgtCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGAc                                                                                    <  2:82544/143‑1 (MQ=255)                                                     tcttctCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTCTGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCACTCACAAGACCGc                                                                                 >  2:582969/1‑143 (MQ=255)                                                            aacaaATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGCTGCCAATCACACGACCGCTTccccc                                                                          >  1:89090/1‑142 (MQ=255)                                                            aacaaATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCg                                                                          >  1:6139/1‑143 (MQ=255)                                                            aacaaATTACTGCTTGCTATGGTCAGCCATAATATTGATAATAAGACTCATTGATATATCAATTTTTATTAATTTTTATGAGTTATACGGATAACAGAAAACTCACGCCTGAAACGGATGCCAATCCCAAGACCGCTTCCCCg                                                                          >  1:621270/1‑143 (MQ=255)                                                              caaTTTGCTGCTTGCTCTGGTCATCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCa                                                                        <  1:204535/143‑1 (MQ=255)                                                                aaTTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAACACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCGGCAGc                                                                      >  1:417259/1‑143 (MQ=255)                                                                aaTTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGc                                                                      >  1:314742/1‑143 (MQ=255)                                                                 aTTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGcc                                                                     >  1:60118/1‑143 (MQ=255)                                                                      tgcttgctCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGGCAATCACAAGACCGCTTCCCCGCAGCCTATTc                                                                >  2:309490/1‑143 (MQ=255)                                                                        cttgctCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCgg                                                              <  2:35053/143‑1 (MQ=255)                                                                        cttgctCTGGTCAGCCATAATATTGATAATAAGAAGCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCgg                                                              <  2:958300/143‑1 (MQ=255)                                                                         ttgctCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGc                                                             <  1:615170/143‑1 (MQ=255)                                                                             tctGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCa                                                                        >  2:990926/1‑128 (MQ=255)                                                                             tctGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCa                                                                        <  1:990926/128‑1 (MQ=255)                                                                              ctGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAAtg                                                        >  2:1016431/1‑142 (MQ=255)                                                                              ctGGTCAGCCATAAGATTGATAAGAAGAATCATTGTTATATCAATTATTATTAACTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAAtt                                                        <  1:866823/143‑1 (MQ=255)                                                                                  tCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGCCGAATTTcca                                                    >  2:415435/1‑143 (MQ=255)                                                                                      ccATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAg                                                                       >  1:521322/1‑120 (MQ=255)                                                                                      ccATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAg                                                                       <  2:521322/120‑1 (MQ=255)                                                                                       cATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTCATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATGTccagccag                                               >  1:838372/1‑143 (MQ=255)                                                                                       cATAATATTGAGTATTAGAAGAATTGTTGTATCAAGTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTccagccag                                               <  2:125609/143‑1 (MQ=255)                                                                                        ataataTTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGa                                              <  2:533880/143‑1 (MQ=255)                                                                                              tggATAATAAGAATCATGGTTATATCAAGTATTATTAATTTTTATGAGTTATACGGATAGCAGAACACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGt                                        <  2:431808/141‑1 (MQ=255)                                                                                               tGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGtt                                       <  1:1016326/143‑1 (MQ=255)                                                                                                 ataataAGAATCTTTGTTATATCAACTATTATTAATTTTTGTGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAg                                     <  2:965846/143‑1 (MQ=255)                                                                                                   aataaGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACCGTTAGGt                                   >  1:75598/1‑143 (MQ=255)                                                                                                     taaGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGTCGAATTTCCAGCCAGACACTGTTAGGTcc                                 <  2:462655/143‑1 (MQ=255)                                                                                                      aaGAATCATTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCg                                <  1:380855/143‑1 (MQ=255)                                                                                                             aTTGTTATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCCCGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAACCAGACCCTCTTAGGTCCGGATGGCa                         >  2:83099/1‑143 (MQ=255)                                                                                                                 ttATATCAATTATTATTAATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACt                     >  2:139980/1‑143 (MQ=255)                                                                                                                     atCAATTATTATTAAGTTGTATGAGTTATGTGGATCGCCTGAAACCCACCCCGGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCGATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGAtt                 <  2:806997/143‑1 (MQ=255)                                                                                                                           tattattaATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGAt           <  2:60118/143‑1 (MQ=255)                                                                                                                              tattaATTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCAt        <  1:989862/143‑1 (MQ=255)                                                                                                                                   aTTTTTATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGACAATCACAAGACCGCTTCCCCGCAACCTATTCGGAGAATTTCCAGCCAGACACGGCTAGGGCCGGATTGCAATCGGATTATCCCGCATGACgg   >  2:609332/1‑143 (MQ=255)                                                                                                                                    tttttATGAGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACCGTTAGGTCCGGATGGCAAACTGAATAAGGAACATGACggg  >  2:1075533/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                       TCTGAATGCGTGCCCATTCCTGACGGAATGGGCATTTCTGCGCAACTTGTTGTCTTCTCAACAAATTACTGCTTGCTCTGGTCAGCCATAATATTGATAATAAGAATCATTGTTATATCAATTATTATTAATTTTTATGCGTTATACGGATAGCAGAAAACTCACGCCTGAAACGGATGCCAATCACAAGACCGCTTCCCCGCAGCCTATTCGGCGAATTTCCAGCCAGACACTGTTAGGTCCGGATGGCAAACTGATTATCGATCATGACGGG  >  NC_000913/1789477‑1789750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑